Method for extracting glutamic acid from glutamic acid fermentation liquor by bipolar membrane electroosmose process

A bipolar membrane and electrodialysis technology, applied in the direction of microorganism-based methods, fermentation, chemical instruments and methods, etc., can solve the problems of environmental pollution, huge equipment, large acid consumption, etc., and achieve no environmental pollution, operation Convenience and cost reduction effect

Inactive Publication Date: 2009-11-18
INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The ion exchange method currently used in our country often needs to use acid to regenerate the resin, the equipment is huge, the operation is complicated, the acid consumption is large, and the waste

Method used

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  • Method for extracting glutamic acid from glutamic acid fermentation liquor by bipolar membrane electroosmose process

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] a. Using Cory nebacterium glutamicum, in the fermentation tank, according to the raw material ratio (weight percentage): starch hydrolyzed sugar 7.5%, corn syrup 0.3%, magnesium sulfate (MgSO 4 ·7H 2 O) 0.03%, disodium hydrogen phosphate (Na 2 HPO 4 ) 0.085%, urea 0.85%, potassium chloride (KCl) 0.01%. In the fermentation process, the inoculum amount of bacteria was 0.8%, 90.425% of water was added, the aeration and stirring fermentation, the temperature was controlled at 30°C, the pH value was 6.5, and the fermentation broth of glutamic acid was obtained after 20 hours of fermentation;

[0018] b. After the glutamic acid fermentation broth obtained in step a is neutralized with sodium hydroxide (NaOH), a sodium glutamate solution is obtained, and the strain and macromolecular proteins are retained in the membrane by ultrafiltration, and the small molecular glutamate Sodium oozes through the membrane to obtain a 2.39% sodium glutamate solution.

[0019] c. Perform bipolar m...

Embodiment 2

[0021] The producing bacteria is Brevibacterium, according to the ratio of raw materials: starch hydrolyzed sugar 8.5%, corn steep liquor 0.6%, magnesium sulfate (MgSO 4 ·7H 2 O) 0.06%, disodium hydrogen phosphate (Na 2 HPO 4 ) 0.17%, urea 1.7%, potassium chloride (KCl) 0.03%, the amount of bacteria inoculated during the fermentation process is 1%, add water 87.94%, in the fermentation tank, aeration and stirring fermentation, temperature control at 34 ℃, pH 6.8 After 30 hours of fermentation, it was neutralized with NaOH, and a solution containing 4.30% sodium glutamate was obtained by ultrafiltration. The bipolar membrane electrodialysis is used for separation, activated carbon is decolorized, and concentrated and crystallized to obtain the finished glutamic acid.

Embodiment 3

[0023] The producing bacteria is Corynebacterium beijing, according to the raw material ratio: starch hydrolyzed sugar 8.5%, corn syrup 0.45%, magnesium sulfate (MgSO 4 ·7H 2 O) 0.045%, disodium hydrogen phosphate (Na 2 HPO 4 ) 0.10%, urea 1.5%, potassium chloride (KCl) 0.015%, the bacterial inoculum in the fermentation process is 0.9%, add water 88.49%, in the fermentation tank, aeration and stirring fermentation, temperature control at 34 ℃, pH 7.0 After 28 hours of fermentation, it was neutralized with NaOH, and a solution containing 4.17% sodium glutamate was obtained by ultrafiltration. The bipolar membrane electrodialysis is used for separation, activated carbon is decolorized, and concentrated and crystallized to obtain the finished glutamic acid.

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Abstract

The invention discloses a method for extracting glutamic acid from glutamic acid fermentation liquor by bipolar membrane electroosmose process. The method includes the following steps: a. microbacterium is used in a fermentation cylinder for the fermentation of proportioned raw materials of hydrolysate of the starch sugar, corn steep liquor, magnesium sulfate, disodium hydrogen phosphate, ureophil and potassium chloride, the steps of strain inoculation, water adding, air agitation fermentation and a certain temperature and pH value maintenance are carried out in a fermentation process, and glutamic acid fermentation liquor can be obtained after a certain period of fermentation; b. after being neutralized by sodium hydroxide, the glutamic acid fermentation liquor obtained in step a is processed by ultrafiltration to remove bacterial strain, protein and other impurities to obtain sodium glutamate liquor; and c. the glutamic acid obtained after the bipolar membrane electrodialysis separation of the sodium glutamate obtained in step b is processed by active carbon decolorization and concentrative crystallization to obtain the finished product. The method has the advantages of simple process, low energy consumption, small volume of equipment and easy operation, the rate of conversion can be as high as 99 percent, the byproduct-dilute sodium hydroxide solution can be circularly utilized, environmental pollution is not generated, and the energy consumption is lower.

Description

Technical field [0001] The invention relates to the technical field of bipolar membrane electrodialysis of organic acids and amino acids, and more particularly to a method for extracting glutamic acid by bipolar membrane electrodialysis in glutamic acid fermentation broth. It is suitable for the separation and purification of organic acids such as gluconic acid, citric acid, tartaric acid, succinic acid and alanine, aspartic acid and glutamic acid in amino acids. Background technique [0002] Fermentation is currently the main method for producing glutamic acid. Using Cory nebacterium glutamicum and Brevibacterium in a fermentor, aeration and stirring fermentation, temperature 30-34 degrees, pH>6.4-7.5, after 20-30h fermentation, the glutamic acid content in the fermentation broth is 4.3 It is difficult to separate and purify due to residual mycelial protein and suspended solids. The method of extracting glutamic acid in the fermentation broth is mainly through the ion exchang...

Claims

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Application Information

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IPC IPC(8): C12P13/14C07C229/24C07C227/40C12R1/15
Inventor 印遇龙吴信黄瑞林
Owner INST OF SUBTROPICAL AGRI CHINESE ACAD OF SCI
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