Method for extracting flavonoid glycoside substance

Abstract

The invention provides a method for extracting flavonoid glycoside substance, comprising the following steps of: taking orange pericarp removed with impurities and dry powder of persimmon leaf as rawmaterials, and extracting with 20-80% alcohol; setting aside, precipitating, taking supernatant, adding diatomite that is 1-5% of supernatant in terms of mass, fully stirring, pressing and filtering by a plate filter, membrane-filtering the filtrate B with PES membrane material, and taking the micro-molecular solution passing through the membrane; putting the obtained micro-molecular solution intoa film evaporator to remove impurities by molecular distillation, collecting the required distillate, performing spray drying, and making into powder to obtain the flavonoid glycoside substance. Themethod for extracting flavonoid glycoside substance has quite simple and convenient operation and low cost, and can save a large amount of solvent, reduce the requirements on the device, also reduce pollution on the environment and well guarantee the natural quality of the flavonoid glycoside substance; the content of flavonoid glycoside component in the produced flavonoid extract is high with a total content of more than 70%, and the transfer rate of general flavone can reach more than 95% with less residual pigment.

Description

technical field

[0001] The invention belongs to a method for extracting, separating and purifying bioflavonoids, a natural drug component, and particularly relates to a method for extracting, separating and purifying from the leaves of Diospyros kaki Thunb, a plant of the family Persimmonaceae, Citrus reticulata Blanco and its cultivars. A new process for separating and extracting flavonoid glycosides from the dried and mature pericarp. The present invention not only relates to extraction with water and ethanol, but also relates to a new process for separating and purifying flavonoid glycosides with diatomite filtration, ultrafiltration, and molecular distillation technology. method. Background technique

[0002] Bioflavonoids (bioflavonoids) are phenolic substances that widely exist in nature, also known as vitamin P, often accompanied by vitamin C, and are secondary metabolites of plants. Flavonoid derivatives of chromanes or chromones, the basic skeleton of which has C ...

Examples

Embodiment 1

[0024] (1). Preparation of raw materials: cleanly select dry citrus peels and dried persimmon leaves produced in Zhejiang Province respectively, remove impurities therein, and after pulverizing, pass through a 20-mesh sieve to obtain citrus peel dry powder and persimmon leaf powder respectively for use;

[0025] (2). Processing of raw materials: Take 0.2 kg of dried citrus peel powder and 0.3 kg of dried persimmon leaf powder according to the mass ratio of citrus peel dry powder and persimmon leaf dry powder in a ratio of 2:3, and add the mass of citrus peel dry powder and persimmon leaf dry powder. 10 times the total amount of 60% ethanol aqueous solution, temperature controlled at 50°C to 60°C, extract for 3 hours, centrifuge and filter to obtain filtrate A, and extract the filter residue twice according to the above method, combine filtrate A to obtain 7.2L of extract;

[0026] (3). Filtration: the extract obtained in step (2) is left to settle, and 7.0 L of the supernatant ...

Embodiment 2

[0031] (1). Preparation of raw materials: cleanly select dry citrus peels and dried persimmon leaves produced in Zhejiang Province respectively, remove impurities therein, and after pulverizing, pass through a 20-mesh sieve to obtain citrus peel dry powder and persimmon leaf powder respectively for use;

[0032] (2). Processing of raw materials: Take 0.3 kg of dried citrus peel powder and 0.45 kg of dried persimmon leaf powder according to the mass ratio of citrus peel dry powder and persimmon leaf dry powder in a ratio of 2:3, and add the mass of citrus peel dry powder and persimmon leaf dry powder. 10 times the total amount of 40% ethanol, temperature controlled at 40°C to 50°C, extracted for 3 hours, centrifuged and filtered to obtain filtrate A, the filter residue was extracted twice according to the above method, and filtrate A was combined to obtain 10.6L of extract;

[0033] (3). The extract obtained in step (2) is allowed to stand for precipitation, and 10.2 L of the su...

Embodiment 3

[0038] (1). Preparation of raw materials: cleanly select dry citrus peels and dried persimmon leaves produced in Zhejiang Province respectively, remove impurities therein, and after pulverizing, pass through a 20-mesh sieve to obtain citrus peel dry powder and persimmon leaf powder respectively for use;

[0039] (2). Processing of raw materials: Take 0.2 kg of dried citrus peel powder and 0.3 kg of dried persimmon leaf powder according to the mass ratio of citrus peel dry powder and persimmon leaf dry powder in a ratio of 2:3, and add the mass of citrus peel dry powder and persimmon leaf dry powder. 12 times the total amount of 50% ethanol, temperature controlled at 35°C to 45°C, extracted for 2.5 hours, centrifuged and filtered to obtain filtrate A, the filter residue was extracted twice according to the above method, and filtrate A was combined to obtain 8.3L of extract;

[0040] (3). The extract obtained in step (2) is allowed to stand for precipitation, and 8.0 L of the sup...

technical field

[0001] The invention belongs to a method for extracting, separating and purifying bioflavonoids, a natural drug component, and particularly relates to a method for extracting, separating and purifying from the leaves of Diospyros kaki Thunb, a plant of the family Persimmonaceae, Citrus reticulata Blanco and its cultivars. A new process for separating and extracting flavonoid glycosides from the dried and mature pericarp. The present invention not only relates to extraction with water and ethanol, but also relates to a new process for separating and purifying flavonoid glycosides with diatomite filtration, ultrafiltration, and molecular distillation technology. method. Background technique

[0002] Bioflavonoids (bioflavonoids) are phenolic substances that widely exist in nature, also known as vitamin P, often accompanied by vitamin C, and are secondary metabolites of plants. Flavonoid derivatives of chromanes or chromones, the basic skeleton of which has C ...

Examples

Embodiment 1

[0024] (1). Preparation of raw materials: cleanly select dry citrus peels and dried persimmon leaves produced in Zhejiang Province respectively, remove impurities therein, and after pulverizing, pass through a 20-mesh sieve to obtain citrus peel dry powder and persimmon leaf powder respectively for use;

[0025] (2). Processing of raw materials: Take 0.2 kg of dried citrus peel powder and 0.3 kg of dried persimmon leaf powder according to the mass ratio of citrus peel dry powder and persimmon leaf dry powder in a ratio of 2:3, and add the mass of citrus peel dry powder and persimmon leaf dry powder. 10 times the total amount of 60% ethanol aqueous solution, temperature controlled at 50°C to 60°C, extract for 3 hours, centrifuge and filter to obtain filtrate A, and extract the filter residue twice according to the above method, combine filtrate A to obtain 7.2L of extract;

[0026] (3). Filtration: the extract obtained in step (2) is left to settle, and 7.0 L of the supernatant ...

Embodiment 2

[0031] (1). Preparation of raw materials: cleanly select dry citrus peels and dried persimmon leaves produced in Zhejiang Province respectively, remove impurities therein, and after pulverizing, pass through a 20-mesh sieve to obtain citrus peel dry powder and persimmon leaf powder respectively for use;

[0032] (2). Processing of raw materials: Take 0.3 kg of dried citrus peel powder and 0.45 kg of dried persimmon leaf powder according to the mass ratio of citrus peel dry powder and persimmon leaf dry powder in a ratio of 2:3, and add the mass of citrus peel dry powder and persimmon leaf dry powder. 10 times the total amount of 40% ethanol, temperature controlled at 40°C to 50°C, extracted for 3 hours, centrifuged and filtered to obtain filtrate A, the filter residue was extracted twice according to the above method, and filtrate A was combined to obtain 10.6L of extract;

[0033] (3). The extract obtained in step (2) is allowed to stand for precipitation, and 10.2 L of the su...

Embodiment 3

[0038] (1). Preparation of raw materials: cleanly select dry citrus peels and dried persimmon leaves produced in Zhejiang Province respectively, remove impurities therein, and after pulverizing, pass through a 20-mesh sieve to obtain citrus peel dry powder and persimmon leaf powder respectively for use;

[0039] (2). Processing of raw materials: Take 0.2 kg of dried citrus peel powder and 0.3 kg of dried persimmon leaf powder according to the mass ratio of citrus peel dry powder and persimmon leaf dry powder in a ratio of 2:3, and add the mass of citrus peel dry powder and persimmon leaf dry powder. 12 times the total amount of 50% ethanol, temperature controlled at 35°C to 45°C, extracted for 2.5 hours, centrifuged and filtered to obtain filtrate A, the filter residue was extracted twice according to the above method, and filtrate A was combined to obtain 8.3L of extract;

[0040] (3). The extract obtained in step (2) is allowed to stand for precipitation, and 8.0 L of the sup...