Method for extracting flavonoid glycoside substance

Abstract

The invention provides a method for extracting flavonoid glycoside substance, comprising the following steps of: taking orange pericarp removed with impurities and dry powder of persimmon leaf as rawmaterials, and extracting with 20-80% alcohol; setting aside, precipitating, taking supernatant, adding diatomite that is 1-5% of supernatant in terms of mass, fully stirring, pressing and filtering by a plate filter, membrane-filtering the filtrate B with PES membrane material, and taking the micro-molecular solution passing through the membrane; putting the obtained micro-molecular solution intoa film evaporator to remove impurities by molecular distillation, collecting the required distillate, performing spray drying, and making into powder to obtain the flavonoid glycoside substance. Themethod for extracting flavonoid glycoside substance has quite simple and convenient operation and low cost, and can save a large amount of solvent, reduce the requirements on the device, also reduce pollution on the environment and well guarantee the natural quality of the flavonoid glycoside substance; the content of flavonoid glycoside component in the produced flavonoid extract is high with a total content of more than 70%, and the transfer rate of general flavone can reach more than 95% with less residual pigment.

Description

technical field [0001] The invention belongs to a method for extracting, separating and purifying bioflavonoids, a natural drug component, and particularly relates to a method for extracting, separating and purifying from the leaves of Diospyros kaki Thunb, a plant of the family Persimmonaceae, Citrus reticulata Blanco and its cultivars. A new process for separating and extracting flavonoid glycosides from the dried and mature pericarp. The present invention not only relates to extraction with water and ethanol, but also relates to a new process for separating and purifying flavonoid glycosides with diatomite filtration, ultrafiltration, and molecular distillation technology. method. Background technique [0002] Bioflavonoids (bioflavonoids) are phenolic substances that widely exist in nature, also known as vitamin P, often accompanied by vitamin C, and are secondary metabolites of plants. Flavonoid derivatives of chromanes or chromones, the basic skeleton of which has C ...

AI Technical Summary

Problems solved by technology

There are many separation and purification methods for flavonoids, such as column chromatography, thin layer chromatography, lead salt precipitation, boric acid complexation, pH gradient extraction, solvent extraction, and high performance liquid chromatography (HPLC) applied in recent years. Analysis (DCCC), gas chromatography, etc., but all have shortcomings in varying degrees that limit their industrial production, and can be used for industrialized supercritical CO 2 The extraction method is suitable for the early stage of the separation process. The adsorption resin method needs complicated steps to achieve the purification of the product, and it is not ideal for the purification of flavonoids.

Molecular distillation technology is non-equilibrium distillation carried out under high vacuum conditions, which can solve a large number of problems that cannot be solved by conventional distillation technology, and is suitable for separating and purifying high value-added components in crude products, and this This kind of separation and purification is difficult to complete by other common separation methods, especially suitable for the extraction and separation of natural substances. It has been successfully used in petrochemical, food, plastic, pharmaceutical and other industries, but it is still limited to the use of fat-soluble substances.