Microbial treatment technology for extracting nicotine

A technology of microbial treatment and nicotine, applied in the direction of organic chemistry, etc., can solve the problems of greater influence of water content on the solubility of tobacco raw materials, high investment and maintenance costs of high-voltage equipment, high requirements for design and manufacturing technology, and solve the problem of pollution discharge , Realize the effect of tobacco resource reuse and good economic benefits

Active Publication Date: 2010-03-17
CHONGQING TECH & BUSINESS UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, ①Steam distillation water is a relatively primitive process for extracting natural nicotine, which is easy to operate, but the processing equipment is huge, the efficiency is low, and the purity of the resulting product nicotine is not high; ②Solvent extraction method has a certain industrial foundation , but when the nicotine is separated from the organic phase into the water phase, an emulsified layer is easily produced on the phase interface during production, resulting in production shutdown, and most of the extraction agents are volatile organic solvents, which are volatile and pollute the environment to a certain extent ; 3. though supercritical fluid extraction has the advantages of strong extraction capacity, simple solvent recovery, energy saving, etc., supercritical fluid CO 2 , the solubility of nicotine is greatly affected by the moisture content of tobacco raw materials, only when the moisture content of tobacco is 25%, CO 2 In order to effectively extract nicotine, and the investment and maintenance costs of high-pressure equipment are large, the design and manufacturing technology requirements are high, the extraction kettle cannot be operated continuously, and the production capacity is low
The limitations of these extraction methods make the production cost of nicotine high, the extraction rate is not ideal, especially the extraction rate of high-purity products is low, and there is a sharp contradiction between "extraction rate, energy consumption and environmental pollution", which is difficult to meet Market demand

Method used

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  • Microbial treatment technology for extracting nicotine
  • Microbial treatment technology for extracting nicotine
  • Microbial treatment technology for extracting nicotine

Examples

Experimental program
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Effect test

Embodiment 1

[0022] A microbial treatment process for extracting nicotine is to use the microbial treatment process to ferment tobacco, which is completed according to the following steps:

[0023] (1) Wash 40g~50g of discarded tobacco leaves, dry them in the air, bake them in an oven at 80°C~110°C for 30min~2hr, take them out, crush them with a pulverizer, and pass them through molecular sieves of 20, 40, and 60 meshes respectively Tobacco leaves with a particle size of less than 60 mesh are obtained, packed in plastic bags, and set aside.

[0024] (2) According to the characteristics of microorganisms degrading plant tissues, the microbial strains of Mycobacterium albicans, Trichoderma korningina, and Pachysomyces were specially selected for inclined plane culture, first-level seed test tube culture, and second-level seed shake flask culture.

[0025] ① Incline cultivation

[0026] Wort yeast culture medium: Take a certain amount of barley malt, crush it, add water 4 times the amount of...

Embodiment 2

[0038] (1), dry the tobacco leaves at 105° C. for 1 hr, pulverize them, sieve through a 60-mesh sieve, select 10 g of tobacco leaves with a particle size less than 60 mesh, and treat them with microwaves with an output power of 900 W and a frequency of 2450 MHz for 30 s;

[0039] (2), choose the white rot fungus and Pachysomyces spp., after cultivating on an inclined plane and culturing the primary seed test tube, inoculate the two cultured strains into a 60ml 250ml Erlenmeyer flask at a ratio of 1:1 for secondary mixing Seed shaker culture, the amount of mixed strains introduced is 5%, 29°C, 180rpm, culture 48hr;

[0040] (3), the tobacco leaf 10g after the pretreatment of step (1) is transferred in the fermenter, adds the soybean meal of 1.5g and 60ml water, inserts the mixed strain that inoculum is 40% tobacco leaf weight in the fermenter, regulates The pH is 5, the temperature is 30°C, and 1ml of the sample is taken after fermentation for 48 hours. After centrifugation, the ...

Embodiment 3

[0043] (1), dry the tobacco leaves at 80° C. for 2 hours, pulverize them, sieve through 20 mesh and 60 mesh sieves, select 10 g of tobacco leaves with a particle size less than 60 mesh, and treat them with microwaves with an output power of 900W and a frequency of 2450mhz for 30s;

[0044] (2), choose the white rot fungus and Pachysomyces spp., after cultivating on an inclined plane and culturing the primary seed test tube, inoculate the two cultured strains into a 60ml 250ml Erlenmeyer flask at a ratio of 1:1 for secondary mixing Seed shaker culture, the amount of mixed strains introduced is 5%, 29°C, 180rpm, culture 48hr;

[0045] (3), the tobacco leaf 20g after the step (1) pretreatment is transferred in the fermenter, adds the soybean meal of 5g and 160ml water, inserts the mixed strain that inoculum is 60% tobacco leaf weight in the fermenter, adjusts pH 7, the temperature was 40°C, 1ml was sampled after 12 hours of fermentation, and after centrifugation, the nicotine con...

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Abstract

The invention discloses a microbial treatment technology for extracting nicotine, which comprises the following steps: (1) pretreatment of tobacco leaves; (2) cultivation of a microbial strain; (3) fermentation of the tobacco leaves; and (4) extraction of the nicotine. The microbial treatment technology adopts the compound microbial treatment for extracting the nicotine, degrades and damages cellulose, hemicellulose and other plant tissues in tobacco after the microwave treatment through white-rot fungus, pachysolen tannophilus and other microbes, effectively releases the nicotine attached onthe tissue structure and improves the dissolution rate and the extraction rate of the nicotine; and the microbial treatment technology can also effectively degrade organic acid, proteins and the likein the tobacco, reduce the emulsification phenomenon during the extraction process of the nicotine and simultaneously reduce the using amount of an organic solvent. The method has the advantages of high extraction rate of the nicotine, a small using amount of the organic solvent, high recovery rate and ability of effectively realizing the reduction of the discarded tobacco and the resource recycling of the tobacco.

Description

technical field [0001] The invention relates to a technology for extracting nicotine, an active ingredient in tobacco, in particular to a technology for extracting nicotine, an active ingredient, by treating tobacco with a microbial fermentation method. Background technique [0002] my country is rich in tobacco resources, with an annual output of 4.5 to 5 million tons of tobacco leaves. In addition to being used for cigarette production, nearly 25% of the leftovers such as tobacco leaves and tobacco powder are discarded, causing environmental pollution. In fact, tobacco is rich in nicotine (1-3%), garnisol (0.3-0.5%), pectin, niacin and many other special natural phytochemicals. Nicotine, also known as nicotine, is an ideal high-efficiency insecticide and biopesticide for the production of green food because of its characteristics of steaming, stomach poisoning, contact killing and rapid degradation without residue. It mainly has the following characteristics: ① Low toxici...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D401/04
Inventor 王星敏殷钟意郑旭煦夏晓芳
Owner CHONGQING TECH & BUSINESS UNIV
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