Medicine composition for treating rheumatoid arthritis and preparation thereof
A rheumatoid and composition technology, which is applied in the direction of drug combination, anti-inflammatory agent, non-central analgesic, etc., can solve the problems of incomplete eradication of lesions and high recurrence rate, and achieve remarkable curative effect, quality controllable, and improved effect Effect
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Embodiment 1
[0029] Example 1: Preparation and detection of total glucosides
[0030] Take 10 kg of unique medicinal material, extract with 10 times the amount of 50% (volume) ethanol three times, each time for 1 hour, collect the extract, concentrate it to about 50L, place it at room temperature, centrifuge, and pass the supernatant through HPD-300 macroporous adsorption resin , first eluted with water until colorless, remove impurities soluble in polar solvents, discard, then elute with 4BV50% (volume) ethanol, collect the ethanol eluate, and dry the eluate after reclaiming ethanol under reduced pressure , that is, total glucosides of unique herb. Based on the total weight of the total glucosides of Solimonas glycosides, the content of total iridoid glycosides is 19.3% (weight), the content of total phenylethanol glycosides is 30.1% (weight), and the content of total flavonoids is 13.8% (weight).
[0031] The content determination method of each component in the total glucosides of Duyi...
Embodiment 2
[0047] Example 2: Preparation and detection of total alkaloids
[0048] Take 10 kg of high aconitum medicinal material, add 3 times the amount of 80% (volume) ethanol to soak for 2 hours, move it to the percolation bucket, add 10 times the amount of 80% (volume) ethanol for percolation, and the percolation speed is 0.5 per hour L, collect the percolation liquid, concentrate under reduced pressure to 2L, adjust the pH value to 10 with 2mol / l sodium hydroxide, stir after standing for 24 hours, continue to stand for 48 hours, centrifuge, take the precipitate and add 500ml of hydrogen with pH10 Sodium oxide solution, fully stirred, centrifuged, again take the precipitate and add 100ml of pH10 sodium hydroxide solution, fully stir, centrifuge, again take the precipitate and add 200ml of pH10 sodium hydroxide solution, fully stir, centrifuge, wash the precipitate with water until neutral, Dry at 100°C to obtain total alkaloids. Based on the total weight of the total alkaloids, the ...
Embodiment 3
[0054] Embodiment 3: Pharmacodynamics test
[0055] (1) The composition of the sample adopted in the pharmacodynamic test is as follows:
[0056] Table 1: The dose grouping table of the total glucosides of Duyiwei, the total alkaloids of Gaowu and the combination of the two
[0057]
[0058] (2) Analgesic test
[0059] 1) Acetic acid writhing test in mice: In this experiment, a negative control group, a positive drug group (tramadol), four dosage groups of total glucosides and total alkaloids alone, and total glucosides and Orthogonal test group of 16 groups of total alkaloids combined. From the experimental results, it can be seen that the combination of total glucosides and high-dose total alkaloids of unicorn all has a significant analgesic effect (results are shown in Table 2), wherein when the weight ratio of total glycosides and total alkaloids of uniquam is 3.37: When 0.75 and 0.84:0.75, Q value>1, showing synergy.
[0060] Table 2: Results of mouse writhing test...
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