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Method for extracting coptisine

An extraction method and a technology for coptisine are applied in the field of biological extraction, can solve the problems of poor chloroform recrystallization safety, difficult to meet large-scale preparation, unfavorable for industrialized production and the like, and achieve the effects of easy implementation, good utilization rate and low cost

Inactive Publication Date: 2010-06-09
SOUTHWEST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sichuan University master's degree thesis, 2007); this process technology needs to use expensive reagent potassium iodide, and the safety of chloroform recrystallization is not good, it is difficult to meet the needs of large-scale preparation
Chinese herbal medicine, 2006, 37(7): 1017); due to the use of preparative liquid chromatography, the equipment is expensive and the investment is large, which is also not conducive to the realization of industrial production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] a) Take 1 kg of Coptidis Rhizoma, pass through a 20-mesh sieve after crushing, then immerse Coptis Rhizoma in 5 liters of sulfuric acid solution with a mass concentration of 1%, heat up to 100° C. and extract for 0.1 hour and then filter to obtain the first acid extract and Residue; in the present embodiment, the concentration of sulfuric acid is 0.05~1%, and the volume is 3-5 times of bulk medicine volume all can realize better acid extraction effect;

[0029] b) According to the method of step a, the residue is acid-extracted three times to obtain the acid-extracted solution of the three-time acid-extracted acid-extracted solution respectively, and the acid-extracted solution obtained for the first acid-extracted and second-time acid-extracted solution is combined and neutralized with milk of lime to pH 10, after standing for 24 hours, discard the precipitation in the acid extract to obtain the supernatant; In this step, the raw materials are subjected to acid extract...

Embodiment 2

[0038] a. Take 1 kg of Cortex Phellodendri, break it through a 20-mesh sieve, then soak Coptidis Rhizome in 15 liters of sulfuric acid solution with a mass concentration of 0.05%, filter it after acid extraction at room temperature for 24 hours, and obtain the first acid extraction solution and residue ;

[0039] b. the residue obtained in step a is acid-extracted with 10 liters of 0.05% sulfuric acid solution, and the extraction conditions are the same as in step a), to obtain the second acid extraction solution and filter residue;

[0040] c. Carry out two acid extractions to the filter residue obtained in step b according to the method of step b again, the first acid extraction and the acid extraction solution of the second acid extraction are combined and neutralized with calcium hydroxide to a pH of 11, and left to stand for 24 After 4 hours, the precipitation in the acid extraction solution was discarded to obtain a supernatant; in the present embodiment, the treatment m...

Embodiment 3

[0049] a. Take 1 kg of the mixture of Coptis chinensis, Cortex Phellodendron and Sanke needles, crush it and pass it through a 20-mesh sieve, then soak the Coptis chinensis in 8 liters of sulfuric acid solution with a mass concentration of 0.3%, raise the temperature of the material to 60°C and extract it with acid for 6 hours Filter to obtain the first acid extract and residue;

[0050] b. The obtained residue was subjected to acid extraction with 5 liters of 0.3% sulfuric acid solution, the acid extraction temperature was 60° C., and the acid extraction time was 2 hours to obtain the second acid extraction solution and filter residue;

[0051] c. Carry out two acid extractions to the obtained filter residue according to the method of step b again, combine the acid extraction solutions of the first acid extraction and the second acid extraction and neutralize with sodium hydroxide to a pH of 12, after standing for 24 hours , discarding the precipitation in the acid extract to...

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Abstract

The invention discloses a method for extracting coptisine, which comprises the following operations: extracting raw materials by acid, neutralizing an acid extract, precipitating to remove impurities, precipitating to remove berberine, regulating the basicity of a solution, separating by a chromatographic column, precipitating, crystallizing and drying a crystal product to obtain a product. The invention takes at least one of coptis root, yellow-corktree bark and radix berberidis as the raw materials, is combined with the physicochemical characteristics of various components in the alkaloid of the coptis root and further realizes the effective separation of jateorrhizine, coptisine and palmatine by the chromatographic operation on the basis of the extraction of the berberine. The method has simple process, easy execution, good repeatability and low cost and can be widely used for the large-scale production of coptisine, and the extraction efficiency of coptisine is high. The purity of the coptisine in the product prepared by the method can reach more than 90%, and the coptisine can be completely used as the raw material of medicines for resisting bacteria, lowering sugar, lowering blood fat, resisting cancer, promoting the power of the stomach and the like and can also be used as the raw material of other products.

Description

technical field [0001] The invention relates to biological extraction technology, in particular to a method for extracting coptisine. Background technique [0002] Coptis chinensis is bitter in taste and cold in nature. It has the functions of purging fire, detoxifying, clearing away heat and drying dampness. It is listed as the top grade in "Shen Nong's Materia Medica". The active ingredients of Coptis chinensis are Coptis chinensis alkaloids, which mainly include: berberine, jatrorrhizine, coptisine and palmatine, etc. , Chinese Journal of Traditional Chinese Medicine, V30(7):594, 2005). Coptisine has good insecticidal activity, can induce nerve cell differentiation, and has anti-cancer, immune, smooth muscle relaxation and anti-nephritis effects. , palmatine and African tetrandrine are strong. [0003] At present, there are many reports on the extraction process of berberine, such as the Chinese patents with application numbers 96105233.3 and 91105606.8. Their technolo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D491/22
Inventor 李学刚叶小利袁吕江
Owner SOUTHWEST UNIV
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