Microbe inoculator with boron nitride film coating and preparation method thereof

A boron nitride film and inoculator technology, which is applied in the method of biological material sampling, coating, metal material coating process, etc., to achieve the effects of low coating stress, small secondary pollution and strong adhesion

Inactive Publication Date: 2010-07-14
JILIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved in the present invention is to use physical and / or chemical coating methods to grow a boron nitride (BN) film coating on the surface of the inoculation wire of the microbial inoculator, improve the service effect and life of the inoculator, and have a simple method, Low cost, no pollution, strong operability, etc., to meet the application in biological experiments

Method used

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  • Microbe inoculator with boron nitride film coating and preparation method thereof
  • Microbe inoculator with boron nitride film coating and preparation method thereof
  • Microbe inoculator with boron nitride film coating and preparation method thereof

Examples

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Comparison scheme
Effect test

Embodiment 1

[0021] Using radio frequency magnetron sputtering method to grow BN film coating on nickel-chromium alloy inoculation wire, there are two processes of pretreatment and BN film coating growth.

[0022] 1) Pretreatment of nickel-chromium alloy seeding wire: use sandpaper to remove the oxide layer on the surface of nickel-chromium alloy seeding wire, immerse it in acetone and ethanol solution and ultrasonically clean it for 5-25 minutes, then ultrasonically clean it with deionized water for 5-25 minutes, Repeat the above cleaning 2 to 3 times until the surface is very clean, take out the nickel-chromium alloy inoculation wire and dry it.

[0023] 2) BN film coating growth: the nickel-chromium alloy inoculation wire is placed flat on the sample stage of the sputtering deposition equipment, and high-purity hexagonal boron nitride (hBN) is used as the target material, (the purity of the target material is preferably 99.8%, The diameter can be 50 mm), and the deposition chamber is ev...

Embodiment 2

[0027] The alloy seeding wire in Example 1 was replaced with platinum seeding wire, and a BN film coating was grown on the platinum seeding wire by radio frequency magnetron sputtering.

[0028] Method is with embodiment 1.

Embodiment 3

[0030] A magnetically enhanced arc plasma (CVD) method is used to grow a BN film coating on a nickel-chromium alloy inoculated wire. There are two processes of pretreatment and BN film coating growth.

[0031] The pretreatment of nickel-chromium alloy seeding wire: with embodiment 1.

[0032] BN film coating growth process: place the nickel-chromium alloy seed wire on the sample stage of the radio frequency magnetron sputtering deposition equipment, N 2 , Ar, H 2 and BCl 3 The mixed gas is the reaction gas, and the gas flow ratio is N 2 :Ar:H 2 :BCl 3 =98:400:100:2, the working pressure is 2-50Pa, the bias voltage is 120V to assist the deposition, the arc discharge current is 10-15A, the deposition time is 30-120 minutes, and the BN film coating is grown on the surface of the nickel-chromium alloy seed wire, The thickness is about 0.5 microns.

[0033] To obtain a BN film coating with a thickness of 0.05-5 microns, the growth time of the BN film can be controlled at 15-9...

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Abstract

The invention relates to a microbe inoculator with a boron nitride film coating and a preparation method thereof, and belongs to the technical field of biological appliances. The microbe inoculator consists of a handle and an inoculation wire (2) made of a platinum material or an alloy material; and the boron nitride film coating (1) is grown on the surface of the inoculation wire (2). The preparation method comprises the following steps of: using the pretreated inoculation wire (2) as a substrate and hexagonal boron nitride as a target material; and growing the boron nitride film coating (1) on the surface of the inoculation wire (2) by adopting a physical deposition method or a chemical vapor deposition method such as megnetron sputtering, vacuum evaporation, ion plating or pulsed laser deposition. The coating prepared by the invention has low stress, has no crack on the surface, has strong adhesion with the inoculation wire, and is not peeled after being placed in air for long time; multiple-burning experiments show that the BN film coating does not fall off; a sintered and carbonized biological product is easy to wash off from the inoculation wire, is convenient to wash, has light secondary pollution, and can be repeatedly used.

Description

technical field [0001] The invention belongs to the technical field of biological instruments. It particularly relates to a microbial inoculator with a coating of functional film material growing on the surface of an inoculation wire and a preparation method thereof. Background technique [0002] The process of inserting microorganisms into artificial medium or living organisms suitable for its growth and reproduction is called inoculation. Microbial inoculators are widely used in biological experiments, and are widely used in laboratories and biological manufacturing plants. The most commonly used microbial inoculator in practice consists of an inoculation wire and a handle. Due to different inoculation requirements or methods, inoculation filaments are roughly divided into inoculation loops and inoculation needles. The inoculation loop is to bend the top of the inoculation silk into shapes such as circular rings and triangular rings; the inoculation needle is to make th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/26C23C14/06C23C16/34C23C14/35C23C16/513
Inventor 李红东刘钧松徐洋杨旭昕李志慧邹广田
Owner JILIN UNIV
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