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Nano-bionic material for tissue repair and preparation method thereof

A nano-bionic and tissue repair technology, applied in the field of biomedicine, can solve problems such as high cost of autologous transplantation, limited sources of autologous materials, and dysfunction

Active Publication Date: 2010-09-15
MEDPRIN REGENERATIVE MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The common disadvantages of both are: the source of autologous materials is limited, and clinical application is often delayed; secondary lesions often occur at the donor site; wounds are treated with injuries, requiring a second operation, resulting in new trauma defects in the donor site; Relatively complex procedure; prone to complications and postoperative scarring; can lead to unhealed or dysfunctional donor sites; and autografts are often expensive
[0025] However, bioprinting technology is still in the stage of basic research. What kind of material is the bio-paper sheet, what is the specific composition of the cell or / and cytokine bio-ink, and how to realize the stacking are all theoretical discussions However, there is no specific nano-bionic material that can be successfully prepared by bioprinting directly using cells, and no relevant technical reports have been seen.

Method used

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  • Nano-bionic material for tissue repair and preparation method thereof
  • Nano-bionic material for tissue repair and preparation method thereof
  • Nano-bionic material for tissue repair and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0074] Preparation of Nano-bionic Wound Covering

[0075] (1) Preparation of electrospinning solution, drug-containing hydrosol solution and cross-linking agent solution:

[0076] For the electrospinning solution, hydrophobic L-polylactic acid and ε-caprolactone are selected, and the ratio of the two is 50:50. As a copolymerized polymer material, the number average molecular weight is 260,000, and it is soluble in hexafluoroisopropanol.

[0077] The cross-linking agent solution is 0.1M calcium chloride solution.

[0078] The hydrosol solution containing cytokines adopts the hemostatic factor alginate solution, and the mass percent concentration of the hemostatic factor in the cytokine alginate solution is 10 ppm.

[0079] Put the prepared 0.1M calcium chloride solution into a cell culture dish with a diameter of 150 mm, and place it on the flat receiver shared by the electrospinning device and the printer. Refit the Hewlett-Packard 550C inkjet printer according to the existi...

Embodiment 2

[0092] The nanomaterial of the embodiment 1 of the present invention---nanometer bionic wound covering (artificial skin), see appendix figure 1 shown, with figure 1 It shows that different concentrations and different types of cytokines are distributed in the nano-bionic scaffold structure, among which 1 is a cell chemokine, 2 is a nanofiber, and 3 is a cell differentiation factor.

[0093] Present embodiment provides the experiment of described artificial skin in repairing rabbit (vegetable rabbit) skin wound:

[0094] Artificial full-thickness skin defect on the abdomen (3×3cm 2 ) adult rabbits were used as experimental animal models. 2 hours after the wound, the nanomaterial artificial skin (3 × 3cm) prepared in Example 1 was 2 ) transplanted to the wound site. After 6 hours of transplantation, it can be seen that the stem cells began to migrate into the artificial skin in a directional way; the graft was well integrated with the normal skin tissue and closely adhered t...

Embodiment 3

[0096] The preferred number-average molecular weight of the electrospinning solution is a dichloromethane solution of poly-DL-lactic acid and polycaprolactone of 26000; the weight percentages of the poly-DL-lactic acid and polycaprolactone are respectively 50% and 50%. 50%, the total mass percent concentration of the solution is 10%.

[0097] The crosslinking agent solution is selected from 100 mg / ml water-soluble carbodiimide solution;

[0098] The hydrosol solution containing cytokines adopts collagen and polyanion solution of cytokines, wherein the collagen concentration is 1%, polyanion adopts polyglutamic acid with a concentration of 50 mg / ml, and the cytokines include cell directional migration factor SDF-1, The total mass percent concentration of epidermal growth factor, transforming growth factor beta and BMP is 0.5%.

[0099] The specific operation is to put the prepared carbodiimide solution into a cell culture dish with a diameter of 150mm, and put it on the flat rec...

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Abstract

The invention provides a nano-bionic material for tissue repair and a preparation method thereof. The nano-bionic material comprises a nano-bionic bracket and hydrosol attached to the nano-bionic bracket, wherein one or more cell factors and / or cells are coated in the hydrosol. The preparation method of the nano-bionic material comprises the following steps of: preparing an electrospinning solution and a hydrosol solution containing the cell factors and / or the cells; preparing the nano-bionic bracket by using the electrospinning; printing the hydrosol solution containing the cell factors and / or the cells on the nano-bionic bracket by using a ink-jet printer; and the like, and repeating the electrospinning and the ink jet to obtain the nano-bionic material with different thicknesses. By combining with an electrospinning technology and a biological printing technology, the invention combines specific medicines and / or the cell factors and / or the cells in the nano-bracket and / or the surface of the nano-bracket, thereby greatly enhancing the effects of the nano-bionic material on tissue repair and tissue regeneration; in addition, the invention has broad application prospect.

Description

technical field [0001] The invention relates to a nano-bionic material for tissue repair and a preparation method thereof, belonging to the technical field of biomedicine. Background technique [0002] Various medical artificial materials are many at present. [0003] When the human body is subjected to external unfavorable factors (such as fire, car accident, impact) or due to its own reasons (such as tumor erosion, congenital diseases), it can cause loss and damage of various tissues or organs, such as: dura mater defect, joint defect, skin Trauma, heart valve insufficiency. Therefore, human tissues and organs basically have a certain self-repair ability [0004] Although different tissues and organs of the human body have certain repairing abilities, their repairing abilities are different. For example, skin regeneration ability is strong, but articular cartilage can hardly repair itself; but once the damage exceeds the necessary limit and scope, the self will Cannot b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/38A61L27/54A61L27/52A61L27/26A61L27/24A61L27/22A61L27/20A61L27/18A61L27/16A61L27/00
Inventor 袁玉宇徐弢
Owner MEDPRIN REGENERATIVE MEDICAL TECH
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