Method for preparing simplified high-purity bulleyaconitine A

Abstract

The invention relates to a method for preparing simplified high-purity bulleyaconitine A, which comprises the following steps of: immersing tuberous root of kusnezoff monkshood root in 1 to 2 percent alkaline solution; crushing the tuberous root; performing heat reflux extraction with gasoline or petroleum ether 6 and the like; loading the extract; eluting the tuberous root with solvent gasoline, ethyl acetate and triethylamine or diethylamine in a volume ratio of 90-95 to 1-10 to 1-5; concentrating the eluent under reduced pressure till the eluent is dry; then thermally dissolving the eluent with the solvent gasoline; standing the obtained solution for 20 to 24 hours at normal temperature; crystallizing, filtering and drying the solution to obtain the crude product of the bulleyaconitine A; fully dissolving the crude product in methanol on a water bath of 45 to 55 DEG C; filtering the obtained solution and preserving the heat for 30 minutes on the water bath of 45 to 55 DEG C; adding pure water or distilled water dropwise to assist in the crystallization; standing the solution for 20 to 24 hours at normal temperature to form a crystal; performing filtration to remove the liquid part; washing the crystal for three times; and pumping the crystal and drying the crystal to obtain the finished product. The process has the advantages of simplified production flow, low cost, no three-waste emission, simple operation, high production safety and high extraction rate of the bulleyaconitine A.

Description

technical field [0001] The invention relates to a process method for extracting effective components from Chinese medicinal materials, in particular to a method for extracting aconitin from the tuber root of a kind of plant Aconitum aconitum. technical background [0002] Bulleyaconitione A has a molecular formula of C35H49O10N and a molecular weight of 643.77. It is a colorless prismatic crystal or white powder. Soluble in ether, chloroform, ethanol, dilute acid water, insoluble in water, has strong analgesic and obvious anti-inflammatory effects. Experiments have proved that the analgesic effect of this product is central and closely related to the level of serotonin in the brain. The onset time is slower than that of morphine (37.8 minutes on average), but the maintenance time is longer (9.3 hours on average). Non-addictive. Its anti-inflammatory effect does not pass through the adrenal system, but is related to the suppression of PG levels; this product also has antipy...

AI Technical Summary

Problems solved by technology

There are problems such as low content, high production cost, high production safety risk, etc., and it is difficult to industrialize large-scale production

In the patent of CN1054976A, the method of semi-synthesizing aconitine from aconitine is disclosed. In actual operation, it is difficult to separate and purify the impurities produced by the reaction, and it is difficult to separate and obtain qualified starting raw material aconitine, which leads to high production cost and also Difficult to industrialize large-scale production