The invention discloses a method for preparing high-purity
bulleyaconitine A. The method comprises the following steps of: soaking a fresh
radix aconiti agrestis root in acid
aqueous solution, performing homogenate extraction and
cavitation mixing
solid-liquid extraction, collecting and concentrating the filtrate, and respectively performing
gradient elution to obtain a crude
bulleyaconitine A product through
ion exchange resin
column chromatography and macroporous adsorption resin
column chromatography; and uniformly mixing crude
bulleyaconitine A product and
ethanol aqueous solution, dissolving in a water bath, cooling the filtrate to
room temperature, standing and crystallizing at the temperature of 4 DEG C, repeatedly washing the
crystal by using the
ethanol aqueous solution, and obtaining a final product. According to the method, crushing and extracting are finished in one step through a homogenization method, and the method has the advantages of zero dust and simple flow. By adoption of a negative-pressure
cavitation extraction technology, the
mass transfer rate is improved, heating is not required, and the production period is shortened; and moreover, the reagents used in the whole process are
ethanol and water, the
organic solvent amount is greatly reduced, the production cost can be reduced, the environmental
pollution is reduced, and the method has good application prospect.