Process for producing fuel ethanol by utilizing bamboo biomass waste

A technology of biomass waste and fuel ethanol, which is applied in the direction of biofuels, microorganism-based methods, microorganisms, etc., can solve the problems of high planting costs, large power consumption, and high processing costs, and achieve reduced production costs, reduced production costs, The effect of simple production process

Inactive Publication Date: 2010-10-13
BIOGAS SCI RES INST MIN OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The above-mentioned various raw materials and production methods have the following disadvantages: 1. The production of fuel ethanol from starchy raw materials generally uses corn, wheat, potatoes and other grain crops or non-grain crops as raw materials, which are easily limited by arable land and production. The use of food crops to produce fuel ethanol is prohibited, which limits the energy utilization of starch raw materials
2. The production of fuel ethanol from carbohydrates mainly adopts sugarcane, sugar beet and sweet sorghum, etc. The planting cost is relatively high, and the regionalization is more obvious. For example, sugarcane is mainly suitable for the southern region of my country, and sugar beet and sweet sorghum are mainly suitable for the northern region.
3. The production of ethanol from crop stalks has mainly focused on corn stalks, but there are still problems such as high transportation and collection costs and low conversion efficiency. At present, my country is still in the research stage
4. At present, H is generally used before cellulose 2 SO 4 And gas explosion and other treatment processes, the conditions are more intense, the power consumption is larger, and the treatment cost is higher
5. The cellulose degradation products are relatively complex, especially containing more five-carbon sugars (such as xylose), and most of the ethanol fermentation strains cannot utilize xylose or produce more by-products (such as xylitol, etc.). Therefore, the ethanol fermentation efficiency is low

Method used

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  • Process for producing fuel ethanol by utilizing bamboo biomass waste
  • Process for producing fuel ethanol by utilizing bamboo biomass waste
  • Process for producing fuel ethanol by utilizing bamboo biomass waste

Examples

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Effect test

Embodiment 1

[0037] A process for producing fuel ethanol from bamboo biomass waste comprises the following steps: after pretreatment of bamboo biomass waste, fuel ethanol is obtained by hydrolysis and fermentation.

[0038] The pretreatment includes: a. Put the bamboo biomass waste at 50 ℃ H 3 PO 4 In the solution, a conventional constant temperature shaking culture shaker was used for 0.5 hours at a speed of 90 rpm, and the bamboo biomass waste was mixed with H. 3 PO 4 The solid-liquid volume ratio of the solution is 1:8; b. Add 2.5 times H 3 PO 4Pre-cooled acetone of the solution volume, stir and mix evenly, and then solid-liquid separation; c. Use 5 times H 3 PO 4 The solution volume of acetone is washed, and then solid-liquid separation is performed, and the precipitate is collected; d, the precipitate is washed with water to obtain a cellulose component.

[0039] In steps b and c, the supernatant is collected after solid-liquid separation to obtain lignin, acetic acid, acetone a...

Embodiment 2

[0048] A process for producing fuel ethanol from bamboo biomass waste comprises the following steps: after pretreatment of bamboo biomass waste, fuel ethanol is obtained by hydrolysis and fermentation.

[0049] The pretreatment includes: a. Put the bamboo biomass waste at 50 ℃ H 3 PO 4 In the solution, the conventional constant temperature shaking culture shaker was used for 1 hour at 90 rpm, and the bamboo biomass waste was mixed with H. 3 PO 4 The solid-liquid volume ratio of the solution is 1:8; b. Add 2.5 times H 3 PO 4 Pre-cooled acetone of the solution volume, stir and mix evenly, and then solid-liquid separation; c. Use 5 times H 3 PO 4 The solution volume of acetone is washed, and then solid-liquid separation is performed, and the precipitate is collected; d, the precipitate is washed with water to obtain a cellulose component.

[0050] The hydrolysis is as follows: adding the pretreated cellulose component to pH 4.8, 0.1M sodium citrate buffer, then adding 2.0-4...

Embodiment 3

[0056] A process for producing fuel ethanol from bamboo biomass waste comprises the following steps: after pretreatment of bamboo biomass waste, fuel ethanol is obtained by hydrolysis and fermentation.

[0057] The pretreatment includes: a. Put the bamboo biomass waste at 50 ℃ H 3 PO 4 solution, treated at 90 rpm for 0.8 h, bamboo biomass waste was mixed with H 3 PO 4 The solid-liquid volume ratio of the solution is 1:8; b. Add 2.5 times H 3 PO 4 Pre-cooled acetone of the solution volume, stir and mix evenly, and then solid-liquid separation; c. Use 5 times H 3 PO 4 The solution volume of acetone is washed, and then solid-liquid separation is performed, and the precipitate is collected; d, the precipitate is washed with water to obtain a cellulose component.

[0058] In steps b and c, the supernatant is collected after solid-liquid separation to obtain lignin, acetic acid, acetone and H 3 PO 4 of the mixture.

[0059] In step d, the precipitate is washed with water, a...

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Abstract

The invention discloses a process for producing fuel ethanol by utilizing bamboo biomass waste, which comprises the following step of carrying out pretreatment on the bamboo biomass waste and preparing the fuel ethanol by hydrolysis and fermentation. The process adopts the bamboo biomass waste as the raw material, can produce a large amount of fuel ethanol with low cost, improves ethanol fermentation rate, reduces production cost of the ethanol and has simple production process and high output value. Meanwhile, the process of the invention adopts mild treatment conditions to realize the process of stage treatment of the raw material so as to realize full utilization of the raw material. Meanwhile, an adopted fermentation strain has high utilization efficiency of glucose and high ethanol yield.

Description

technical field [0001] The invention belongs to the field of industrial biotechnology, and relates to a process method for producing fuel ethanol, in particular to a process for producing fuel ethanol from bamboo biomass waste. Background technique [0002] The existing raw materials for producing fuel ethanol from biomass resources include starch raw materials, sugar raw materials, potato raw materials, and crop straw raw materials. For example, Chinese Patent No. "200480011192.0" discloses a method for producing ethanol from plant material, which was filed on March 10, 2004 and published on May 31, 2006, comprising pulverizing the plant material to produce a starch-containing material; comminuted plant material having a particle size suitable for passing at least about 50% of the particle size through a sieve having 0.1-0.5 mm mesh openings; saccharifying the starch without cooking with an enzyme composition; fermenting the incubated starch to produce A composition compri...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/10C12R1/01
CPCY02E50/16Y02E50/10
Inventor 何明雄胡启春祝其丽潘科
Owner BIOGAS SCI RES INST MIN OF AGRI
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