Method for performing steam explosion of wood fiber raw material, directional enzymatic dissociation and alcoholic fermentation

Abstract

The invention relates to a method for performing steam explosion of wood fiber raw materials, directional enzymatic dissociation and alcoholic fermentation. The method is characterized in that steam explosion, directional enzymolysis of xylan, solid-liquid separation, extensive enzymolysis of cellulose, anaerobic alcoholic fermentation of xylose and anaerobic alcoholic fermentation of glucose areadopted. The new secondary enzymolysis technology is adopted and beta-glucosidase and little cellulose are introduced in xylanase so as to obviously improve the xylan directional enzymolysis and separation effect of raw materials. Therefore, the invention provides a new method for the effective separation of xylose and glucose in the wood fiber raw materials and the efficient alcoholic fermentation and significantly reduces the production cost for the fermentation of the ethanol in wood fiber raw materials.

Description

1. Technical field [0001] The invention belongs to the technical field of enzymatic hydrolysis and biotransformation of biomass raw materials in biochemistry, and in particular relates to a method for preparing fuel ethanol through steam explosion pretreatment of lignocellulosic raw materials, directional enzymolysis of xylan, xylose fermentation and glucose fermentation. 2. Background technology [0002] Cellulose and xylan in lignocellulosic raw materials can be hydrolyzed to glucose and xylose, and then fermented by microorganisms to produce many bio-based platform compounds, such as fuel ethanol, organic acids and polyols. Among many lignocellulosic raw materials, agricultural and forestry waste straws and grasses are rich in glucosyl and xylose, with huge yield and low price, which is the focus of the current development of biomass raw materials. [0003] In straw and grass natural raw materials, cellulose, xylan and lignin form a tight complex through complex chemical ...

AI Technical Summary

Problems solved by technology

These strains used for xylose fermentation have low tolerance to sugar and fermentation products such as ethanol during the fermentation process, and the utilization of xylose is inhibited by glucose, and precise restrictions need to be controlled during the xylose fermentation process Sexual oxygen supply measures

Due to the different fermentation strains and fermentation process conditions of these two types of sugars, the industrial scale-up of the synchronous fermentation technology of hexose and pentose sugars in the enzymatic hydrolysis liquid of lignocellulosic raw materials is seriously restricted, and there are problems such as high manufacturing and operating costs of fermentation equipment, wind loss Large size, complex process control, low yield and many other deficiencies have formed the bottleneck of industrial production

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