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Beta-cypermethrin degrading bacteria and application thereof

A technology of high-efficiency cypermethrin and degrading bacteria, which is applied in the direction of bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve rare problems such as low cost, good application prospects, and convenient use Effect

Inactive Publication Date: 2010-11-10
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on efficient-cypermethrin-degrading bacteria and microbial selective degradation of efficient-cypermethrin

Method used

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  • Beta-cypermethrin degrading bacteria and application thereof
  • Beta-cypermethrin degrading bacteria and application thereof
  • Beta-cypermethrin degrading bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1: Screening and identification of strains

[0021] Medium:

[0022] Inorganic salt medium: NaCl 1g, K 2 HPO 4 1.5g, KH 2 PO 4 0.5g, (NH4) 2 SO 4 1.5g, MgSO 4 0.1g, 1ml trace element solution (1L trace element solution is prepared according to the following composition: MnSO 4 ·H 2 O 0.13g, ZnCl 2 0.23g, CuSO 4 ·H 2 O 0.03g, CoCl 2 ·6H 2 O 0.42g, Na 2 MoO 4 2H 2 O 0.15g, AlCl 3 ·6H 2 O 0.05g, made up to 1000ml with distilled water, made up to 1000ml with distilled water, mixed and stirred evenly, natural pH value, prepared after high-pressure steam sterilization (121°C, 20min).

[0023] Enrichment culture solution: add beta-cypermethrin solution to the inorganic salt culture solution so that the concentration of beta-cypermethrin is 50 mg / L.

[0024] LB liquid medium: Yeast powder 10g, peptone 5.0g, sodium chloride 10.0g, distilled water to make up to 1000ml, mixed and stirred evenly, natural pH value, prepared after high-pressure steam...

Embodiment 2

[0031] Embodiment 2: bacterial agent preparation

[0032]1. Inoculate the strains stored in the liquid test tube in 40ml of inorganic salt culture solution for activation and culture for 4 days;

[0033] 2. Inoculate the activated bacteria into 100ml LB liquid medium containing 50mg / L, shake at 30°C and 150rpm until logarithmic growth phase;

[0034] 3. Centrifuge (6000rpm) the above-mentioned bacterial solution in the logarithmic growth phase for 3 minutes, discard the supernatant, and suspend the bacterial cells with an appropriate amount of phosphate buffer solution with a pH value of 7.0, which is the microbial agent.

[0035] The formula of 0.2mol / L phosphate buffer solution with a pH of 7.0 is: take 39ml of 0.2mol / L sodium dihydrogen phosphate and 61ml of 0.2mol / L disodium hydrogen phosphate, dilute to 1000ml with ultrapure water, and steam under high pressure It can be obtained after sterilization (121°C, 20min).

Embodiment 3

[0036] Embodiment 3: Beta-cypermethrin degradation experiment

[0037] Detection of beta-cypermethrin content in inorganic salt culture solution:

[0038] Use the whole bottle extraction method to extract beta-cypermethrin, add an equal volume of dichloromethane into the conical flask containing 40ml of enrichment solution, shake it fully and let it stand for 3h, take 5ml of the lower organic phase into a clean test tube, add anhydrous sodium sulfate Dehydration, after passing through a 0.45μm filter membrane, draw 1ml of liquid into the sample bottle, dry it under nitrogen protection, dilute it to 1ml with chromatographic grade n-hexane, and store it in a refrigerator at 4°C for HPLC analysis .

[0039] Beta-cypermethrin (beta-cypermethrin, β-CP) has two chiral centers, containing 4 enantiomers, and four peaks will appear on the chiral HPLC spectrum, which can be detected by examining the ER before and after degradation (for Enantiomer ratio) value changes to determine whet...

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Abstract

The invention provides a beta-cypermethrin degrading bacteria-nitrogen-fixing vibrio (Azoarcus Indigens) HZ5, which is collected at the China center for type culture collection, wherein the address is Wuhan University of Luojia hill, Wuhan, Hubei, 430072, the collection data is March 17, 2010, and the collection number CCTCC No is M 2010060. The bacteria of the invention has certain degradation capacity to high-concentration beta-cypermethrin, the degradation rate for the 50mg / L of beta-cypermethrin under pure culture conditions reaches 65.2%, and the enantioselectivit does not exist in the degradation process. The degrading bacteria can be applied to the degradation of the beta-cypermethrin in a water body by direct adding, and can safely, efficiently and fast degrade the residual beta-cypermethrin on the water body, soil and other objects; and the bactericide containing the bacterial strain has simple preparation process, low cost, convenient use, and good application prospect.

Description

(1) Technical field [0001] The invention relates to an efficient cypermethrin degrading bacterium—Azoarcus indigens HZ5 and application thereof. (2) Background technology [0002] Pyrethroid pesticides have been used in my country for more than 50 years. With the prohibition of highly toxic and refractory pesticides such as organophosphorus and organochlorine, the production and use of pyrethroid pesticides have been expanded. Now pyrethroids Esters pesticides account for more than one-third of the total application area of ​​pesticides in my country, mainly for the control of cotton, fruit trees, vegetables and household pests. Pyrethroid pesticides have stomach poisoning and contact killing effects. Although they are safe for crops, they are moderately toxic to humans and animals, and highly toxic to aquatic organisms, bees, and silkworms. In 1997, Lin Xiaotao and others discovered that fenpropathrin was highly toxic to Macrobrachium rosenbergii; in 1999, Zhu Lusheng et al...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/38
Inventor 马云江吉红袁泉东邱吉国商弘颖刘维屏
Owner ZHEJIANG UNIV OF TECH
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