Method and device for recovering and/or enriching aqueous-phase mobilized lipases

A technology for immobilizing lipase and lipase, applied in the direction of hydrolase and the like, can solve the problems of increased energy consumption during evaporation and reflux, high preparation cost of immobilized enzyme, complicated processing process, etc., and achieves low recovery energy consumption and no enzyme. The effect of life loss and low investment in equipment

Active Publication Date: 2011-04-20
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are some unavoidable disadvantages in the preparation of biodiesel by chemical method: ① The free fatty acid and water in the oil raw material seriously affect the progress of the reaction; ② The solubility of methanol in oil is not good, which easily leads to the formation of emulsion and complicates the subsequent treatment process ; ③ The process requires that the amount of methanol used greatly exceeds the reaction molar ratio, and the evaporation and reflux of excess methanol increases the energy consumption of the process
However, the preparation cost of immobilized enzymes is very high, and most of the costs are attributed to the downstream process operation costs of enzymes, that is, the separation, purification and immobilization of enzymes.

Method used

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  • Method and device for recovering and/or enriching aqueous-phase mobilized lipases
  • Method and device for recovering and/or enriching aqueous-phase mobilized lipases
  • Method and device for recovering and/or enriching aqueous-phase mobilized lipases

Examples

Experimental program
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Effect test

Embodiment 1

[0036] The lipase is produced by microbial fermentation, the enzyme activity of the lipase in the fermentation broth is 2000IU / ml, and the pH is 6.5. The foam separation device used in this embodiment can be found in figure 2 As shown, the specific details are as follows.

[0037] Through the external liquid feed pump 2-8, 4L fermentation liquid is pumped into the foam separation device from the foam column feed port 2-7 on the side wall of the foam column 2-4, and the external gas compression pump 2- 9 Pass the air into the foam tower 2-4 at a gas velocity of 10L gas / L liquid / h, and the gas distributor 2-10 provided at the bottom of the foam tower 2-4 distributes the air into tiny bubbles to generate foam, and the foam layer is When the height reaches twice the height of the liquid layer, start to collect the foam through the discharge port 2-5 on the side wall of the foam tower and the peripheral side line foam collector 2-1 connected thereto, and pass through the piston i...

Embodiment 2

[0040] The lipase is produced by microbial fermentation, the enzyme activity of the lipase in the fermentation broth is 2000IU / ml, and the pH is 6.5. The foam separation device used in this embodiment is the same as that in Embodiment 1.

[0041] Pour 4L of fermentation broth into the foam separation device. Air is introduced at a gas velocity of 10L gas / L liquid / h to generate foam. When the height of the foam layer reaches twice the height of the liquid layer, start to collect the foam, and always keep the height of the foam layer at about twice the height of the liquid layer. The total ventilation time was 90 minutes, and the volume of foam liquid and enzyme activity were measured every 15 minutes. The lipase activity of the resulting foam liquid at 15, 30, 45, 60, 75 and 90 minutes was respectively 7000IU / ml, 6000IU / ml, 4500IU / ml, 4000IU / ml, 3700IU / ml and 3500IU / ml; Enzyme enrichment ratios were 3.5:1, 3:1, 2.25:1, 2:1, 1.35:1 and 1.25:1; foam volumes were 0.17L, 0.53L, ...

Embodiment 3

[0043] 4L of lipase (Rhizopus oryzae free lipase) solution with a concentration of 100 μg / ml was adjusted to pH 5.0 with sulfuric acid and then poured into a foam separation device. The foam separation device used in this embodiment can be found in figure 2 As shown, the specific details are as follows.

[0044] Through the liquid feed pump 2-8 of the peripheral equipment, put 4L fermented liquid into the foam separation device from the foam tower feed port 2-7 on the side wall of the foam tower 2-4, and pass the gas compression pump 2- 9. Air is passed into the foam tower 2-4 at a gas velocity of 5L gas / L liquid / h, and the gas distributor 2-10 provided at the bottom of the foam tower 2-4 distributes the air into tiny bubbles to generate foam. After the foam rises to the top foam collector 2-1, it flows out from the top outlet 2-3 through the top guide tube 2-2. The output was collected and the aeration was stopped after 2 hours. The foam liquid is obtained after the foam ...

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Abstract

The invention discloses a method and a device for recovering and/or enriching aqueous-phase mobilized lipases. In the method provided by the invention, a gas is introduced into solution to be recovered and/or enriched, and the lipases in the solution are recovered and/or enriched by foam separation; and the method is particularly applicable to the recovery and/or enrichment of the aqueous-phase mobilized lipases in the bioenzymic production of biodiesel. The method provided by the invention can effectively recover and enrich the lipases in an aqueous phase and keep the activity of the lipases, has the advantages of low energy consumption and low cost, and is easy to amplify and operate. In addition, the invention also provides the device used for the method. The device can be used independently, or a plurality of devices can be connected in series for use so as to realize batch type or continuous operations.

Description

technical field [0001] The invention belongs to the field of biochemical industry, and relates to a method and device for recovering non-immobilized lipase in water phase, in particular to a method and device for recovering non-immobilized lipase in water phase in the process of producing biodiesel by using foam separation, recovery and / or enzymatic enrichment in water phase The invention relates to a method and a device for producing lipase in a fermentation liquid in the process of producing lipase by an enzyme or fermentation method, so as to realize the recovery and enrichment of non-immobilized lipase and use it for the production of biodiesel. Background technique [0002] Due to the depletion of fossil energy and the carbon dioxide, nitrogen oxide, sulfur oxide and black smoke emitted after combustion have caused serious environmental pollution problems, the development of renewable alternative energy sources, such as bioenergy, has attracted widespread attention from ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/16
Inventor 赵雪冰杜伟刘德华
Owner TSINGHUA UNIV
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