Preparation method for myostatin knock-out pig

A technology of myogenesis inhibition and gene knockout, applied in the field of genetic engineering, can solve the problems of less research on genetic engineering, and achieve the effects of difficult technical safety, simple operation, and high practical value in production
CN102021201AInactive Publication Date: 2011-04-20NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2011-04-20
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a preparation method for a myostatin knock-out pig, belonging to the technical field of biology. The preparation method is as follows: generating virus particles capable of expressing shRNA of myostatin gene of a targeted pig in a 293Ft cell; then infecting the fibroblasts of a piglet by using the produced slow virus; wherein after 48 hours, the expression of Myostatin genes in the fibroblasts is inhibited by fluorescence quantitative polymerase chain reaction (PCR) detection. In the invention, the slow virus particles can be used for carrying out microinjection on perivitelline space of a fertilized egg of the pig, can lead the virus nucleic acid to be integrated to an embryo gene group so as to express the shRNA, and can produce a transgenic pig with the myostatin of being knocked out after embryo transplantation. The slow virus particles also can be used for producing the transgenic pig by using a sperm vector method. By utilizing the preparation method, the cumbersome operation of knocking out the homologous targeting gene in the traditional method can be avoided, the virus consumption is less and the stable passage can be achieved.
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Description

technical field

[0001] The invention relates to a method for preparing myostatin gene knockout pigs by using lentiviral vector to mediate RNAi, and belongs to the technical field of genetic engineering. Background technique

[0002] my country is the world's largest pig-raising country and the largest pork consumer. But what is not commensurate with it is that the modern pig breeding work is relatively backward, so far there is no modern commercial lean meat pig breed that is fully domesticated (Zhen Yunxiao. Pork import and export situation and countermeasures [J]. China Animal Husbandry Communication, 2003, (12); Wang Aiguo. Review and prospect of my country's pig industry [J]. Contemporary Animal Husbandry, 2004, (01).). Therefore, cultivating high lean meat rate and grain-saving pig breeds is a major demand of the country, and it is also a key and difficult point in the field of pig genetics and breeding. The development of transgenic and gene knockout technology provid...

Claims

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