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Composite leavening agent combining xylanase with multiple strains and method for fermenting straw feed

A technology of compound starter and xylanase is applied in the field of xylanase combined with multi-strain fermentation to prepare straw feed, which can solve the problems of high starter cost, large number of strains, waste of resources and the like, and achieve pH stability. Good, stable activity, low cost effect

Inactive Publication Date: 2011-05-11
天津科建科技发展有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

About 65% to 80% of the dry matter in crop straw can provide energy for animals, but currently less than 10% is used as feed, and most of the crop straw is still directly returned to the field or used as fuel, which causes waste of resources and pollution. surroundings
However, microbial fermentation of straw faces three major difficulties. First, straw fermentation generally requires the use of more than a dozen microorganisms to work together. The number of strains is large, and the synergy between each other is difficult to achieve the desired effect. The cost of starter is too high; second, it is difficult to breed suitable For strains with high cellulase production in straw substrate fermentation, once the production of cellulase is low, it will affect other microorganisms and fail to achieve the best fermentation effect; third, it is necessary to solve the problem of degradation of end products in the process of straw fermentation. Enzyme Synthesis and Its Activity Produce Feedback Inhibition Problems

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0119] 1. Production of solid xylanase

[0120] Strain cultivation: Aspergillus niger (Aspergillus niger) is used as the production strain and adopts a test tube slant strain → shake flask scale-up culture method.

[0121] Solid-state mechanical fermentation: Enlarged cultivation by seed tanks, secondary fermentation in solid-state mechanical fermentation tanks, control the temperature in the tank at 28-30°C, control the water content of fermentation materials: 60-70%, control the air humidity at 75%-85%, and ventilate 8 ~12m 3 / h, tank pressure 0.01~0.02Mpa, inoculum size 5~10%, fermentation time 72~96 hours; medium used in solid fermentation tank (g / Kg): corncob powder (through 20 mesh sieve) 300~350 grams , 120-140 grams of bran, 4-6 grams of corn steep liquor, NH 4 NO 3 2-4 grams, K 2 HPO 4 3-5 grams, MgSO 4 ·7H 2 O0.4-0.6 g, 800-1000 ml of water, pH 5.0, sterilized at 121°C under three atmospheres for 60 minutes.

[0122] Purification: add a certain amount of wate...

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Abstract

The invention discloses a composite leavening agent combining xylanase with multiple strains and a method for fermenting a straw feed. The leavening agent consists of the following components in part by weight: 4 parts of xylanase, 2 parts of lactobacillus acidophilus, 2 parts of propionibacterium, 2 parts of saccharomyces cerevisiae, 3 parts of bacillus subtilis, 1 part of lactobacillus plantarum and 1 part of bacillus coagulans, wherein the xylanase can quickly degrade cell walls of straw plants and release nutrient substances; and under the synergistic effects of a plurality of microorganisms, fermentation speed is increased, fermentation period is shortened, fermentation activity is enhanced, protein conversion rate is increased and fermentation cost is lowered. The prepared fermented straw feed has low cellulose content, high protein content, high viable count, long retention period, soft and wet feel, strong acid incense, high palatability and high nutritional value. The problem of low resource utilization ratio of crops such as straws and the like is solved; simultaneously, environmental pollution is reduced; and the leavening agent and the method have high economic benefit and social benefit.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, and relates to a compound starter for fermenting straw feed and a new method for preparing straw feed by combining xylanase with multi-strain fermentation. Background technique [0002] At present, human beings are facing global problems such as resource scarcity, energy shortage and environmental pollution. Many countries are actively exploring and in-depth research on new energy sources. Biomass energy has always been an important energy source for human survival. In the entire energy system occupies a rather important position. my country is a large agricultural country with abundant crop straw resources, with an annual output of 556 million tons. About 65% to 80% of the dry matter in crop straw can provide energy for animals, but currently less than 10% is used as feed, and most of the crop straw is still directly returned to the field or used as fuel, which causes waste of r...

Claims

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Application Information

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IPC IPC(8): A23K1/14A23K10/12A23K10/18A23K10/30A23K20/189
Inventor 揣玉多杨扬马志刚王德培
Owner 天津科建科技发展有限公司
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