Composite leavening agent combining xylanase with multiple strains and method for fermenting straw feed
A technology of compound starter and xylanase is applied in the field of xylanase combined with multi-strain fermentation to prepare straw feed, which can solve the problems of high starter cost, large number of strains, waste of resources and the like, and achieve pH stability. Good, stable activity, low cost effect
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[0119] 1. Production of solid xylanase
[0120] Strain cultivation: Aspergillus niger (Aspergillus niger) is used as the production strain and adopts a test tube slant strain → shake flask scale-up culture method.
[0121] Solid-state mechanical fermentation: Enlarged cultivation by seed tanks, secondary fermentation in solid-state mechanical fermentation tanks, control the temperature in the tank at 28-30°C, control the water content of fermentation materials: 60-70%, control the air humidity at 75%-85%, and ventilate 8 ~12m 3 / h, tank pressure 0.01~0.02Mpa, inoculum size 5~10%, fermentation time 72~96 hours; medium used in solid fermentation tank (g / Kg): corncob powder (through 20 mesh sieve) 300~350 grams , 120-140 grams of bran, 4-6 grams of corn steep liquor, NH 4 NO 3 2-4 grams, K 2 HPO 4 3-5 grams, MgSO 4 ·7H 2 O0.4-0.6 g, 800-1000 ml of water, pH 5.0, sterilized at 121°C under three atmospheres for 60 minutes.
[0122] Purification: add a certain amount of wate...
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