Separation and purification method for liposome containing encapsulated material

A purification method and liposome technology, applied in the field of biomedicine, can solve the problems of time-consuming and labor-intensive effects, phospholipid raw material consumption, long-term and other problems, and achieve the effects of simple operation, high-efficiency recovery, and wide application range
CN102113998BInactive Publication Date: 2013-11-06PEKING UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
PEKING UNIV
Publication Date
2013-11-06
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for separating and purifying liposome containing encapsulated material through gel electrophoresis and belongs to the field of biomedicine. The method comprises the following steps: firstly preparing liposome containing encapsulated drug, then preparing polymer gel, and separating the liposome from non-encapsulated drugs by gel electrophoresis. Compared with the prior art, the invention has the following characteristics: (1), based on the difference in migration of different components under the action of an electric field, the method achieves the effective separation of the liposome from the non-encapsulated drugs; (2), with the polymer gel as the medium, the method can separate the liposomes of different sizes and different types from the non-encapsulated drugs by changing the pore size of the gel; (3), the method has a wide application range and is applied to the separation of small molecule drugs as well as DNAs, siRNAs and proteins; and (4), the method simplifies the separation operation and achieves the high-efficiency recovery of the liposome and the non-encapsulated drugs after separation.
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Description

technical field

[0001] The invention relates to the separation and purification of liposomes containing drugs, belonging to the field of biomedicine. Background technique

[0002] Liposome drugs have received more and more attention in the field of modern medicine, and are also playing an increasingly important role. Liposome-encapsulated drugs can not only effectively improve the biological activity of drugs, but also improve and regulate the absorption and release of drugs through targeted methods, prevent the destruction of sensitive drugs, reduce side effects, and so on. As a drug carrier, liposomes can encapsulate hydrophilic and hydrophobic drugs, proteins and nucleic acids, and have been widely used in many biomedical fields such as cancer treatment, antibacterial, vaccine and gene therapy.

[0003] The common method of encapsulating drugs in liposomes is to mix drugs during liposome preparation, or encapsulate drugs by fusion after liposome formation. According to ...

Claims

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