Injectable biological active factor composition and preparation method and application thereof

A bioactive factor and composition technology, which is applied to the injectable bioactive factor composition and the fields of its preparation and application, can solve the problems of reducing the strength of hydrogels, and achieves the improvement of gel strength, easy availability of materials, and shortening of gels. The effect of setting time

Inactive Publication Date: 2011-08-03
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Crompton K.E. et al. (Crompton K.E., et al.Biomaterials, 2007, 28:441-449.) reduce chitosan/ The isotonic poin

Method used

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  • Injectable biological active factor composition and preparation method and application thereof
  • Injectable biological active factor composition and preparation method and application thereof
  • Injectable biological active factor composition and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0046] Chitosan is dissolved in the acetic acid solution, is mixed with 3% chitosan solution, slowly adds in the oil phase liquid paraffin containing emulsifier (the emulsifier is the mixture of Span-80 and Tween-80, both volume ratios are 2 : 1, the volume ratio of emulsifier and oil phase is 1: 100.), stirring to form a W / O emulsion, adding 20% ​​(w / w) sulfuric acid solution for cross-linking, forming sulfuric acid cross-linked chitosan microspheres, and then Wash with acetone, absolute ethanol and distilled water, respectively. Then sulfuric acid cross-linked chitosan microspheres are dispersed in water, and the sodium hydroxide solution with a concentration of 2% (w / v) is added dropwise under slow stirring until the final pH value of the reaction system is 7.6; After fully washing with ethanol and water, the chitosan microspheres are obtained after freeze-drying. figure 1 It is the infrared spectrogram of chitosan microspheres, which shows that the ion-crosslinked chitosa...

Embodiment 2

[0048] The chitosan was fully dissolved in 0.08 mol / L hydrochloric acid solution and the insoluble matter was filtered off to prepare a 1.8% (w / w) chitosan solution (pH value 5.8). Dissolve 10 mg of recombinant human bone morphogenetic protein-2 in 10 mL of chitosan solution, then slowly drop 0.5 mL of 45% (w / w) sodium glycerophosphate solution at 4°C to form an isotonic solution (300 mOsm); then Slowly add 50mg of chitosan microspheres into the above homogeneous solution to make recombinant human bone morphogenetic protein-2 composition, its pH value is 7.0, it is a flowable liquid at room temperature, increase the temperature to 37°C for 5 minutes form stable semi-solid gels, such as image 3 shown. Using scanning electron microscope to observe the dry gel, the results are as follows: Figure 4 As shown, the pore size of the xerogel is between 100-200 μm.

Embodiment 3

[0050] Firstly, 2-week-old Kunming mice were anesthetized by intraperitoneal injection of pentobarbital sodium at 3% of their body weight, fixed on the operating board in supine position, shaved and disinfected the posterior side of the right thigh, and injected with 1 mL syringe containing 100 μg of recombinant Composition 100 μL of human bone morphoprotein-2. After the operation, put them into the feeding box and feed them with normal clean standard feed. The mice were sacrificed 4 weeks after the operation, and one of the mice was quickly taken out of the implanted material and its surrounding soft tissue, immersed in 10% formalin solution for fixation, sectioned, and hematoxylin-eosin staining to observe the osteogenesis. observation, such as Figure 5 As shown, bone tissue, including trabecular bone and bone marrow, was found in the striated muscle tissue. The other 3 mice dissected their right legs to remove new long bones, as Image 6 As shown, the average fresh weig...

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Abstract

The invention discloses an injectable biological active factor composition and a preparation method thereof. The biological active factor composition comprises one or more than one cell growth factor with biological activity, an isotonic chitosan solution and chitosan microspheres suspending in the solution, and temperature-dependent gelatinization is carried out to form hydrogel. The preparation method of the biological active factor composition comprises the following steps of: preparing the isotonic chitosan solution, and adding biological active factors and chitosan microspheres to form the biological active factor composition. The biological active factor composition can be applied to the defective parts of bones or cartilages in an injection mode, the hydrogel is formed in situ at the physiological pH value and temperature, and biological active factors are slowly released.

Description

technical field [0001] The invention relates to an injectable bioactive factor composition and its preparation method and application. Background technique [0002] Growth factors are a class of biologically active factors that exist in the body, have a variety of regulatory functions, and play an important role in the repair and regeneration of body tissues. However, biologically active factors have a very short half-life in the body and will be quickly diluted by tissue fluid, transported, Degraded and unable to function. Therefore, the function of biologically active factors depends on an appropriate carrier so that they can be released slowly in the body. [0003] Hydrophilic gel has a good adsorption effect on protein macromolecules, which can play a role of slow release and prolonged effect, which is beneficial to the play of biologically active factors. Among the numerous hydrogels, there is a temperature-sensitive hydrogel material that is in a solution state at lo...

Claims

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Application Information

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IPC IPC(8): A61K38/18A61K38/30A61K47/36A61P19/08
Inventor 刘昌胜钱秀珍王靖
Owner EAST CHINA UNIV OF SCI & TECH
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