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Chitosan and sulfating grifolan mixed gel freeze-dried sponge, and preparation method and application thereof

A Grifola frondosa polysaccharide and sulfation technology, which is applied in biomedicine and natural polymer fields, can solve problems such as insufficient flexibility, poor cell compatibility, and poor hydrophilicity, and achieve low cost, wide source of materials, The effect of good biosafety and degradability

Inactive Publication Date: 2012-07-25
NANTONG WANGZI HOUSEHOLD ARTICLES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Because the performance of pure chitosan freeze-dried sponge-like material is lamellar structure, it has certain mechanical strength but its flexibility is insufficient; in addition, because of its poor hydrophilicity, the cell compatibility is not good

Method used

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  • Chitosan and sulfating grifolan mixed gel freeze-dried sponge, and preparation method and application thereof
  • Chitosan and sulfating grifolan mixed gel freeze-dried sponge, and preparation method and application thereof
  • Chitosan and sulfating grifolan mixed gel freeze-dried sponge, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Slowly add 40 grams of Grifola frondosa polysaccharide (polysaccharide content > 30%) into 30 mL of chlorosulfonic acid, cool in an ice-water bath, and stir at 800 rpm at high speed. After 40 minutes, the reaction solution was slowly poured into 50 mL of refrigerated 2mol / L NaOH, and the temperature was controlled not to exceed 40°C. Adjust the pH value to neutral, precipitate the aqueous solution with absolute ethanol, and filter. The precipitate was dissolved in 20 mL of deionized water, dialyzed, and freeze-dried to obtain sulfated Grifola frondosa polysaccharide with a degree of substitution of 100%.

[0032] 1.6 grams of chitosan (molecular weight: 800,000, degree of deacetylation: 90%) was dissolved in 80 mL of 2% ascorbic acid aqueous solution with high-speed stirring, 0.4 grams of sulfated Grifola frondosa polysaccharide was dissolved in 20 mL of deionized water to prepare a solution, and 0.0001 grams of chitosan was added. Genipin, stir to mix evenly, pour int...

Embodiment 2

[0034] Slowly add 20 grams of Grifola frondosa polysaccharide (polysaccharide content > 30%) into 15 mL of concentrated sulfuric acid, cool in an ice-water bath, and stir at a high speed of 1000 rpm. After 60 minutes, slowly pour the reaction solution into 30 mL of refrigerated 1mol / L Na 2 CO 3, the control temperature does not exceed 45°C. Adjust the pH value to neutral, precipitate the aqueous solution with absolute ethanol, and filter. The precipitate was dissolved in 20 mL of deionized water, dialyzed, and freeze-dried to obtain sulfated Grifola frondosa polysaccharide with a degree of substitution of 90%.

[0035] 1.2 grams of chitosan (molecular weight 1 million, degree of deacetylation 92%) was dissolved in 75 mL of 2% malic acid aqueous solution with high-speed stirring, 0.8 grams of sulfated Grifola frondosa polysaccharide was dissolved in 25 mL of deionized water to prepare a solution, and 0.005 gram of polyethylene glycol diglycidyl ether, stirred and mixed evenl...

Embodiment 3

[0037] Slowly add 30 grams of Grifola frondosa polysaccharide (polysaccharide content > 20%) into 20 mL of concentrated sulfuric acid, cool in an ice-water bath, and stir at a high speed of 500 rpm. After 20 minutes, slowly pour the reaction solution into 30 mL of refrigerated 1mol / L Na 2 CO 3 , the control temperature does not exceed 30°C. Adjust the pH value to neutral, precipitate the aqueous solution with absolute ethanol, and filter. The precipitate was dissolved in 10 mL of deionized water, dialyzed, and freeze-dried to obtain sulfated maitake frondosa polysaccharide with a degree of substitution of 80%.

[0038] 0.8 grams of chitosan (molecular weight 600,000, deacetylation degree 90%) was dissolved in 80 mL of 0.2% lactic acid aqueous solution with high-speed stirring, and 1.2 grams of sulfated Grifola frondosa polysaccharide was dissolved in 20 mL of deionized water to prepare a solution. 0.008 g of ethylene glycol diglycidyl ether, stirred and mixed evenly, poured...

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Abstract

The invention relates to chitosan and sulfating grifolan mixed gel freeze-dried sponge, and a preparation method and application thereof. The chitosan and sulfating grifolan mixed gel freeze-dried sponge comprises 20 to 98 mass percent of chitosan and 2 to 80 mass percent of sulfating grifolan mixed gel freeze-dried sponge. The chitosan and sulfating grifolan mixed gel freeze-dried sponge adopts natural animal polysaccharide and plant polysaccharide as raw materials, and has excellent biological safety and degradability, wide material sources and low cost. A microstructure and degrading speedof the gel sponge can be controlled through types and concentration of acid, freezing temperature and using amount of a cross-linking agent and by a freezing-freeze-drying method. The porous sponge material can be applied to a wound hemostatic material, a cardiac valve, endocranium, a deep scalding material and the like and can promote damaged tissues to heal quickly.

Description

technical field [0001] The invention belongs to the technical field of natural macromolecules, and also belongs to the technical field of biomedicine, and specifically relates to a chitosan and sulfated Grifola frondosa polysaccharide blended gel freeze-dried sponge and a preparation method and application thereof. Background technique [0002] Chitosan is also called soluble chitin, deacetylated chitin, chitosamine, and chitosan. It uses shrimp and crab shells as raw materials. It is a natural polymer compound made from the acetyl group, and its content in nature is second only to cellulose. Since the chitosan molecule contains active polar groups such as hydroxyl and amino groups, hydrolysis reactions can occur on the main chain, and the amino group at the C-2 position and the hydroxyl group at the C-6 position can undergo acetylation, glycolylation, Carboxymethylation, cyanoethylation, sulfation, oxidation, xanthation and other chemical modifications. Under the action o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L5/08C08L5/02C08B37/02C08J9/28A61L15/28
Inventor 黄爱宾杨学佳刘彩凤陈壁辉周盛华
Owner NANTONG WANGZI HOUSEHOLD ARTICLES