Xanthobacter sp. D7 capable of degrading dioxane and application thereof

A dioxane and yellow technology, applied in the direction of bacteria, water/sludge/sewage treatment, biological water/sewage treatment, etc., can solve the problem that there are no yellow bacteria to degrade dioxane, etc.

Active Publication Date: 2011-08-31
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] So far, there is no report about th

Method used

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  • Xanthobacter sp. D7 capable of degrading dioxane and application thereof
  • Xanthobacter sp. D7 capable of degrading dioxane and application thereof
  • Xanthobacter sp. D7 capable of degrading dioxane and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Isolation and Identification of Xanthobacter sp.D7

[0024] (1) Sample collection and domestication

[0025] The activated sludge from the sewage treatment tank of a chemical plant in Zhejiang was collected on site, and dioxane was used as the only carbon source and energy source for domestication and enrichment. Several months later, the activated sludge was inoculated into a 250mL sealed saline bottle containing 50mL of BSM medium, and dioxane was used as the only carbon source and energy source to continue cultivation and enrichment. The experiment requires a constant temperature (30±1°C) and is kept under aerobic conditions.

[0026] BSM was formulated as follows: 4.5g Na 2 HPO 4 12H 2 O, 1.0g KH 2 PO 4 , 1.5g NH 4 Cl, 0.2g MgSO 4 ·7H 2 O, 0.023g CaCl 2 , 1mL trace element mother solution, water to make up to 1000mL.

[0027] Concentration composition of trace element mother liquor: FeSO 4 ·7H 2 O 1.0g / L, CuSO 4 ·5H 2 O 0.02g / L, H 3 BO 3 0.014g / L, ...

Embodiment 2

[0033] Example 2: Performance of Xanthobacter sp.D7 (CCTCC NO: M 2010225) in degrading dioxane

[0034] Seed liquid preparation: D7 was inoculated on the R2A slant and cultured for 3 days, then inoculated from the slant into BSM medium containing 100mg / L dioxane and cultured for 2 days, centrifuged at 1200rpm / min for 5min, discarded the supernatant, and washed with normal saline Rinse and wash the bacteria twice to make a bacterial suspension, which is the seed solution for the experiment.

[0035] Using dioxane as the sole carbon source of Xanthobacter sp.D7, inoculate the bacterium from the seed liquid to 50mL BSM medium, so that the initial bacterial cell concentration (in terms of OD) is 0.01; add dioxane to make the initial dioxane concentration 100mg / L. Put it into a shaker with a temperature of 30°C and a rotation speed of 160r / min for cultivation, and take samples at regular intervals, see the results figure 2 . With the prolongation of time, the bacterial cell con...

Embodiment 3

[0040] Embodiment 3: Xanthobacter sp.D7 purifies dioxane waste water

[0041] Inoculate Xanthobacter sp.D7 bacteria solution in the biotrickling filter tower for continuous treatment at a concentration of 100mg / m 3 of dioxane exhaust gas. After 25 days of film-hanging start-up, the purification efficiency of the dioxane waste gas of the bio-trickling filter reached over 93%, and the system has been running stably since then.

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Abstract

The invention provides a novel strain-xanthobacter sp. D7 capable of degrading dioxane and application thereof to the decomposing treatment of dioxane by microorganisms. The xanthobacter sp. D7 is collected in China Center for Type Culture Collection, of which the address is Wuhan University, Wuhan, China and the zip code is 430072, the collection date is Sept. 9th, 2010, and the collection number is CCTCC No: M2010225. The bacteria capable of degrading dioxane are aerobic non-fermented type gram negative bacteria and the diozane can be used as the unique carbon source and energy for breeding and mineralizing the substrate into carbon dioxide and water; under the condition of pure culture, the bacteria can degrade the dioxane at the pH value of between 4.0 to 10 and the temperature of between 25 and 40 DEG C; the strain has relatively high adaptive capacity to environment, can be used for biological repair of polluted environment and lays a foundation for the project of purifying wastewater and waste gas which contain the dioxane by the biological method.

Description

(1) Technical field [0001] The invention relates to a new bacterial strain capable of degrading dioxane - Xanthobacterium D7 and its application. (2) Background technology [0002] Dioxane (1,4-Dioxane), also known as 1,4-dioxane and diacetic anhydride, is a flammable liquid with an ether smell and is highly toxic. It is listed as B2 by the US Environmental Protection Agency (possibly of) human carcinogens. As an important organic chemical solvent, dioxane is used as a solvent, reaction medium, and extraction agent in the manufacture of special fine chemicals such as medicine, cosmetics, and fragrances, as well as in scientific research. In addition, many surfactants also contain dioxane, and these surfactants are often used in various consumer products, such as various household detergents. Dioxane has a strong dissolving ability, and relevant studies have shown that dioxane is an inhibitor of cytochrome enzyme P450, which can enter the body through the respiratory tract,...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B01D53/84
CPCY02A50/20Y02P20/59
Inventor 陈东之陈建孟金小君朱润晔
Owner ZHEJIANG UNIV OF TECH
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