Use of metabolites of nigrospora oryzae 46 in prevention of colletotrichum lindemuthianum
A technology of black sporum oryzae and bean anthracnose, applied in the field of microorganisms
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Embodiment 1
[0020] Determination of the antibacterial activity of Nigeria oryzae 46: Take 1 mL of the fermentation supernatant filtered through a 0.22 μm membrane in a sterile petri dish, and quickly mix it with 9 mL of PDA medium cooled to 50 ° C. After cooling, place on each medium plane Put 1 piece of bacterium cake for testing such as cucumber blight fungus (Rhizoctonia solani) or bean anthracnose fungus (Colletotrichum lindemuthianum) or tomato gray mold fungus (Botrytis cinerea) with a diameter of 4 mm respectively, and the fungus cake is connected to the center of the petri dish (petri dish 9 cm in diameter), placed in an incubator for 72 hours at 28°C, treated with sterile water as a control, and repeated 3 times; the diameter of the colony was measured by the cross method, and the inhibition rate was calculated by the following formula:
[0021]
[0022] The results showed that the inhibitory rate of the metabolites of Nigella oryzae 46 to R. blight of cucumber, Fusarium wilt o...
Embodiment 2
[0025] (1) Activation culture of strains: move the 46 strains of N. oryzae isolated and preserved to a solid plate of potato dextrose PDA, and cultivate them at 28°C±1°C until the hyphae cover the entire petri dish. A 4mm hole puncher is punched to obtain a fungus cake for inoculation;
[0026] (2) Fermentation culture: The fermentation culture medium contains 20g of soybean cake powder, 15g of corn flour, 20g of soluble starch, 10g of bran, 8g of peptone, and NH 4 NO 3 3g, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 1g, 60mL of fermentation broth per 300mL triangular bottle; after preparation, sterilize at 121°C for 20min, cool to 40°C, pick 1 piece of bacteria cake with a diameter of 4mm under sterile conditions, and keep at 28°C±1°C, shaker speed 180 rpm, fermented for 128 hours;
[0027] (3) Extraction of fermented product: After the fermentation is completed, the fermented liquid is centrifuged to collect, add an equal volume of ethyl acetate to extract, remove the lo...
Embodiment 3
[0031] (2) Determination of antibacterial activity: Take the above mother liquor and dilute it into solutions with a concentration of 1500mg / L and 1000mg / L respectively, put 1mL of each concentration solution in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50°C Evenly (the final concentration of the metabolites of Nigrosporum oryzae 46 is 150mg / L and 100mg / L), after cooling, put a 4mm-diameter bean anthracnose or cucumber blight or tomato Botrytis cinerea respectively on each medium plane The fungus cake, the fungus cake is connected to the center of the petri dish (the diameter of the petri dish is 9cm), placed in an incubator for 36 hours at 28°C, and treated with conventional chemicals and sterile water as a control, repeated 3 times; the diameter of the colony is measured by the cross method , the inhibition rate was calculated by the following formula:
[0032]
[0033] Test example: (product of the present invention and conventional pestic...
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