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Method for producing phytosterol from deodorized distillate of vegetable fat obtained by microbial fermentation

A technology for deodorizing distillate and microbial fermentation, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as environmental pollution, easy inactivation, and difficult to preserve, and achieve low equipment investment and production costs. Low, high throughput effects

Inactive Publication Date: 2012-02-15
HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] 2. The use and consumption of a large amount of toxic and flammable organic solvents such as methanol and acetone, on the one hand, increases the construction investment of the production workshop and increases the risk of safety production; on the other hand, it is not good for the health of workers and causes environmental pollution
[0010] 3. Use lipase to catalyze the conversion of free fatty acids into corresponding methyl esters. Although the reaction conditions are mild and there is no environmental pollution, the price of lipase is high, it is easy to inactivate, and it is not easy to store

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Production of Phytosterols by Microbial Fermentation of Soybean Oil Deodorization Distillate

[0033] a. Microorganisms fermenting vegetable oil deodorization distillates

[0034] The microorganism used to ferment the vegetable oil deodorization distillate is Candida tropicalis.

[0035] b. Medium

[0036] In 1L of water, add 60g of soybean oil deodorized distillate (containing 10.2% phytosterol), 1.0g of yeast powder, 0.5g of magnesium sulfate, 3.0g of dipotassium hydrogen phosphate, and the pH value is 6.5. Weigh the various ingredients and add them into a mechanically stirred fermenter, stir evenly to obtain a deodorized distillate fermentation medium; then put steam into the fermenter to start sterilization, wait until the temperature of the tank rises to 121°C, and keep it warm for 30 minutes . After the sterilization, the temperature of the culture medium was lowered to 28°C.

[0037] c. Fermentation

[0038] Inoculate the Candida tropicalis liquid into the a...

Embodiment 2

[0046] Production of Phytosterols by Microbial Fermentation of Rapeseed Oil Deodorization Distillate

[0047] a. Microorganisms fermenting vegetable oil deodorization distillates

[0048] The microorganism used to ferment the vegetable oil deodorization distillate is Candida tropicalis.

[0049] b. Medium

[0050] In 1L of water, add 65g of rapeseed oil deodorized distillate (containing 20.5% phytosterol), 0.5g of yeast powder, 1.0g of magnesium sulfate, 4.0g of dipotassium hydrogen phosphate, and the pH value is 7.0. Weigh the various ingredients and add them into a mechanically stirred fermenter, stir evenly to obtain a deodorized distillate fermentation medium; then put steam into the fermenter to start sterilization, wait until the temperature of the tank rises to 121°C, and keep it warm for 30 minutes . After the sterilization, the temperature of the culture medium was lowered to 30°C.

[0051] c. Fermentation

[0052] The Candida tropicalis liquid was inoculated int...

Embodiment 3

[0060] Microbial Fermentation of Cottonseed Oil Deodorization Distillate to Produce Phytosterols

[0061] a. Microorganisms fermenting vegetable oil deodorization distillates

[0062] The microorganism used to ferment the vegetable oil deodorization distillate is Candida tropicalis.

[0063] b. Medium

[0064] In 1L of water, add 80g of cottonseed oil deodorized distillate (containing 17.2% phytosterol), 2.0g of yeast powder, 1.0g of magnesium sulfate, 3.5g of dipotassium hydrogen phosphate, and the pH value is 6.5. Weigh the various ingredients and add them into a mechanically stirred fermenter, stir evenly to obtain a deodorized distillate fermentation medium; then put steam into the fermenter to start sterilization, wait until the temperature of the tank rises to 121°C, and keep it warm for 30 minutes . After the sterilization, the temperature of the culture medium was lowered to 30°C.

[0065] c. Fermentation

[0066] Inoculate the Candida tropicalis liquid into the a...

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Abstract

The invention belongs to the field of biochemical industry, and in particular relates to a method for producing phytosterol from deodorized distillate of vegetable fat by microbial fermentation. The method is characterized in that the deodorized distillate of vegetable fat obtained by microbial fermentation is used, the free fatty acid in the deodorized distillate is used as a nutrient substance of microorganism to be directly metabolized and utilized; glyceride in the deodorized distillate is hydrolyzed into micromolecule substances in the presence of hydrolases such as lipase, esterase and the like, and then the micromolecule substances are absorbed and utilized by microorganism cells so as to be transformed to cell substances; phytosterol combined with free fatty acid and glyceride is released to fermentation broth after free fatty acid and glyceride are digested and utilized, the fermentation broth is subjected to microfiltration so that microorganism cells in the fermentation broth are removed; and because phytosterol is not dissolved in water, filtrate is subjected to low temperature crystallization after concentration so as o separate out phytosterol. The method has the advantages of greatly simplifying the production process, reducing energy consumption and reducing production cost; and at the same time, because an organic solvent is not used, the method dose not generate environmental pollution and has obvious economic benefits.

Description

technical field [0001] The invention relates to a method for producing phytosterols from deodorized distillates fermented by microorganisms, and belongs to the technical field of biochemical industry. Background technique [0002] Phytosterols widely exist in plants and plant seeds, and are one of the components of cell membranes in plants. Phytosterols contain sitosterol, stigmasterol, campesterol, and brassicasterol. Phytosterols have good physiological functions, such as lowering serum cholesterol, lowering blood fat, anti-tumor, reducing the incidence of heart disease, preventing prostate diseases, anti-cancer, regulating animal growth, anti-inflammation, anti-virus, and delaying aging. Precursor for the synthesis of various hormones, vitamin D and steroids. Phytosterols also have the characteristics of good emulsification and stability. Therefore, phytosterols are widely used in the fields of food, medicine, cosmetics, animal growth agents and textile, printing and p...

Claims

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Application Information

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IPC IPC(8): C12P33/00C12R1/74
Inventor 赵国群刘金龙
Owner HEBEI UNIVERSITY OF SCIENCE AND TECHNOLOGY
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