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Lactobacillus and fermentation method thereof

A technology of lactic acid bacteria and fermentation method, applied in the biological field, can solve problems such as hindering application and poor stress resistance of lactic acid bacteria

Active Publication Date: 2014-02-19
北京中科景明生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although lactic acid bacteria can improve the immunity and production performance of animals, the application of such bacteria in production practice is hindered due to the poor stress resistance of lactic acid bacteria, such as being sensitive to temperature and highly lethal to oxygen, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The present embodiment is the screening process of the lactobacilli whose preservation number is CGMCC No.3614, and the specific steps are as follows:

[0047] (1) Primary screening of strains

[0048] Using Rogosa SL complete selective medium to select and screen the microorganisms in different positions of the gastric mucosa of piglets to obtain Lactobacillus.

[0049]Wherein Rogosa SL complete selective medium formula is prior art, specifically: tryptone 10g, yeast extract powder 5g, glucose 10g, arabinose 5g, sucrose 5g, sodium acetate 15g, ammonium citrate 2g, potassium dihydrogen phosphate 6g, 0.58g of magnesium sulfate heptahydrate, 0.25g of manganese sulfate tetrahydrate, 0.03g of ferrous sulfate, 1g of Tween 80, 13g of agar, 1000mL of distilled water, and adjust the pH to 5.0 with acetic acid.

[0050] (2), re-screening for gastric acid resistance of Lactobacillus

[0051] Use Rogosa SL medium to carry out anaerobic fermentation on the lactobacillus screened ...

Embodiment 2

[0062] The Lactobacillus sp. (Lactobacillus sp.) preservation number obtained in Example 1: CGMCC No.3614 was inoculated in the anaerobic tube, and the seed medium formula used was: peptone, 10g; Beef extract, 10g; Yeast extract powder, 5g; Dipotassium hydrogen phosphate, 2g; diammonium citrate, 2g; glucose, 20g; sodium acetate, 5g; Tween 80, 1g; The seeds were cultured in an incubator at a temperature of 37° C. for 24 hours to obtain a seed culture. The culture can be stored frozen or directly inoculated.

Embodiment 3

[0064] The seed culture obtained in Example 2 was inoculated in the shake flask culture medium, and the formula of the shake flask culture medium used was: milk powder, 2g; peptone, 1g; beef extract, 0.5g; lactose, 1.5g; yeast extract, 0.5g; Distilled water, 1000mL, adjust the pH to 5.4 with glacial acetic acid. The inoculum ratio was 10%, and cultured in an incubator at a temperature of 37° C. for 12 hours as a shake flask culture.

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PUM

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Abstract

The invention provides a lactobacillus and a fermentation method thereof, which belong to the technical field of biology. The lactobacillus is the lactobacillus sp. preserved in China General Microbiological Center Culture Collection Center on January 27th, 2010, and the accession number in the collection center is CGMCC No.3614, the lactobacillus belongs to the strain separated from the piglet excrement, the strain has the outstanding characteristics that the high-temperature-resistance and oxygen-resistance capability is high, and simultaneously, the strain has stable performance that the gastric acid and cholate resistance effects are realized, the lactic acid is produced, the Escherichia coli growth inhibiting effect is realized, and the like. The viable bacteria number per milliliter after the milk powder and bouillon culture medium fermentation can reach more than 6.2*10<10>.

Description

technical field [0001] The invention relates to a lactobacillus and a fermentation method thereof, belonging to the field of biotechnology. Background technique [0002] The application of antibiotics in feed has created a revolution in aquaculture and feed industry. Large-scale farming has developed rapidly all over the world. Adding antibiotics to livestock and poultry feed has a good effect on improving disease resistance and production performance of livestock and poultry. However, feed The long-term use and abuse of antibiotics in China have serious negative effects. The main manifestations are the emergence of drug resistance of pathogenic bacteria and the residue of antibiotics in animal products. The emergence of antibiotic resistance of pathogenic bacteria has brought great difficulties to the treatment and prevention of human and livestock diseases, and the residues of antibiotics in livestock products have seriously endangered human health after entering the huma...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A61P31/04C12R1/225
Inventor 江生吴连福胥学新边桂萍
Owner 北京中科景明生物技术有限公司