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Citrobacter sp. for removing H2S gas from gas, and use thereof

A Citrobacter, H2S technology, applied in the direction of bacteria, air quality improvement, microbial-based methods, etc., can solve the problems of low proportion of effective microorganisms and difficult control of operating conditions

Active Publication Date: 2014-07-09
QINGDAO SPRING WATER TREATMENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The existing hydrogen sulfide biological treatment technology often inoculates the activated sludge directly on the filler to form a biofilm. The proportion of effective microorganisms in the film is low, and it needs to be domesticated for a long time after the film is formed. Difficult to control

Method used

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  • Citrobacter sp. for removing H2S gas from gas, and use thereof
  • Citrobacter sp. for removing H2S gas from gas, and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Remove H from the gas 2 The method for isolation and culture of Citrobacter sp. (Citrobacter sp.) thioheterotrophs of S gas comprises the steps:

[0017] (1) Sludge was taken from Tianjin Jizhuangzi Sewage Treatment Plant, supplemented with nutrient solution and aerated every day to make SVI above 90mL / g, and complete microbial culture. Stop adding the nutrient solution, add sodium thiosulfate aqueous solution (sulfur ion concentration is 2g / L) every day, keep ventilation, and after 15 days, the desulfurization bacteria become the dominant strain, and the desulfurization bacteria are domesticated.

[0018] Nutrient solution composition: glucose 0.1g, peptone 0.5g, K 2 HPO 4 0.6g, KH 2 PO 4 0.6g, MgCl 2 6H 2 O0.1g, NH 4 Cl 0.2g, a small amount of FeSO 4 7H 2 O and MgSO 4 -5H 2 O, add distilled water to 1000ml.

[0019] (2) Take out 10mL of the mud-water mixture obtained in step (1), add it into an Erlenmeyer flask equipped with 90mL sterilized distilled wa...

Embodiment 2

[0024] Thioheterotrophic bacteria of the present invention has the following microbiological characteristics:

[0025] 1. Morphological properties

[0026] Observed under a microscope, it is rod-shaped, 1.2 μm long and 0.32 μm wide; beige colonies are formed on the culture medium.

[0027] 2. Cultivation characteristics

[0028] The Citrobacter sp. strain of the present invention has the following properties when cultured in a solid medium at 10°C.

[0029] The surface is smooth and wet, the surface is raised, the edges are neat and regular, brown dots appear on the colonies, the individual is round, and the bacteria are beige

[0030] 3. Physiological characteristics

[0031] Gram-negative bacteria that remove phosphorus in sewage under aerobic conditions, positive for catalase, positive for methyl red test, hydrolyzed starch, liquefied gelatin, no indole production, growth pH value of 6-8, and growth temperature of 5-30°C .

[0032] According to the above microbiologica...

Embodiment 3

[0034] Citrobacter sp. Thioheterotrophs on gas H 2 S gas removal effect.

[0035] Inoculate the screened Citrobacter sp. into the culture medium for expanded culture to form a bacterial suspension; soak the ceramsite filter material in it, add the culture medium every day and continue to aerate, the thickness of the biofilm on the surface of the filter material When it reaches 1-2mm, the film hanging is completed; add the filter material with the film hanging to the biological trickling filter layer by layer (see figure 1 ), when the spray liquid flow rate is 8L / h and contains H 2 S air flow 0.4m 3 / h, where H 2 S mass concentration 0.2~0.25mg / m 3 , Start the device under the process conditions of room temperature. Wait for H 2 The removal rate of S has reached more than 85%, and the outlet mass concentration is controlled at 0.03mg / m 3 Below, it indicates that the biotrickling filter unit has been started and has entered a stable operation state.

[0036] After stabil...

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Abstract

The present invention discloses citrobacter sp. for removing H2S gas from gas, and a use thereof. The citrobacter sp. desulfurization heterotrophic bacteria CGMCC No.5285 for removing the H2S gas from the gas has the function of H2S gas removing from the gas. The experimental results show that: with arranging the citrobacter sp. desulfurization heterotrophic bacteria CGMCC No.5285 on a ceramsite filtering material, and filling the resulting material in a bio-trickling tower, the bio-trickling tower can rapidly remove the H2S gas, the mass concentration of the H2S on the H2S outlet is less than 0.03 mg / m<3> after treating so as to meet the factory boundary first class standard in the GB14554-93 emission standards for odor pollutants.

Description

technical field [0001] The present invention relates to the removal of H in gas 2 Citrobacter of S gas and its use. Background technique [0002] h 2 S is an irritating and suffocating colorless gas with a strong foul odor at low concentrations. inhaling H 2 When the S concentration is low, a more obvious stimulating effect can be seen. When the inhaled concentration is high, the olfactory nerve endings are paralyzed, which can stimulate H 2 After the smell disappears, coma or even death occurs. [0003] Traditional hydrogen sulfide treatment methods are not only expensive but also easy to cause secondary pollution. Practice has proved that biological methods are cheap and efficient in purifying air pollution. Among them, the purification of malodorous polluted gas by biological trickling is a relatively new environmental biotechnology, and it is also the most promising waste gas biological treatment technology at present. [0004] When the waste gas with or without pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B01D53/84B01D53/52C12R1/01
CPCY02A50/20
Inventor 于振滨李彦斌宋美芹张晶晶沈旺张伟
Owner QINGDAO SPRING WATER TREATMENT
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