Polylactide electrospinning composite film for in-situ gel encapsulating and preparation method thereof

A technology of polylactide and composite membrane, which is applied in spinning solution preparation, textile and papermaking, non-woven fabrics, etc., to achieve the effects of simple preparation process, controllable drug release rate, and good biocompatibility

Inactive Publication Date: 2012-06-20
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The existing electrospun composite membranes embedding particles are all prepared by the two-step method of firstly preparing the particles and then

Method used

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  • Polylactide electrospinning composite film for in-situ gel encapsulating and preparation method thereof
  • Polylactide electrospinning composite film for in-situ gel encapsulating and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 6mg of CS-SH and 0.4 mg of PEGDA were dissolved in 1 mL of PBS (0.1 M, pH 7.4) as the aqueous phase. Then, 8 mg of F127 was dissolved in a mixed solvent of 0.8 mL of chloroform and 0.2 mL of DMF as an oil phase. Then add 120mg PLGA (LA:GA=3:1, ) is dissolved in the oil phase solution obtained above. Take 40 μL of the above-prepared water phase and disperse it in 1 mL of the above-prepared oil phase, stir (3000r / min) and mix for 5-30 minutes to form a W / O dispersion. The prepared W / O dispersion was electrospun using a single-needle electrospinning device with a voltage of 14kV, a flow rate of the dispersion of 0.4mL / h, and a receiving distance of 18cm for electrospinning. After 12 hours, the collected fibers were electrospun with a diameter of 200nm to 500nm. The composite membrane has a thickness of 40 μm to 70 μm, and the content of chitosan gel is 0.1% to 0.2%. The scanning electron micrographs of the prepared chitosan gel / PELCL electrospun composite membrane ar...

Embodiment 2

[0021] 40 mg of Gtn-SH and 2 mg of PEGDA were dissolved in PBS as the aqueous phase. Then, 5 mg of F127 was dissolved in a mixed solvent of 0.8 mL of chloroform and 0.2 mL of DMF as an oil phase. Further, 120 mg of PLGA was dissolved in the oil phase solution obtained above. Take 40 μL of the above-prepared water phase and disperse it in 1 mL of the above-prepared oil phase, stir (3000r / min) and mix for 5-30 minutes to form a W / O dispersion. The prepared W / O dispersion was electrospun using a single-needle electrospinning device with a voltage of 12kV, a flow rate of the dispersion of 0.3mL / h, and a receiving distance of 20cm for electrospinning. After 12 hours, the collected fibers were electrospun with a diameter of 200nm to 500nm. The composite film has a thickness of 40 μm to 70 μm, wherein the content of gelatin gel is 1% to 2%. Taking HRP as a drug model, the encapsulation rate is above 70% (mass), and the burst release rate is below 20%, which can achieve the purpose ...

Embodiment 3

[0023] 5mg CS-SH and 0.5 mg of PEGDA were dissolved in PBS as the aqueous phase. Then, 10 mg of Span 80 was dissolved in a mixed solvent of 0.9 mL of chloroform and 0.1 mL of DMF as an oil phase. Then 80mg PELCL (LA: CL = 3: 1, ) is dissolved in the oil phase solution obtained above. Take 67 μL of the above-prepared water phase and disperse it in 1 mL of the above-prepared oil phase, stir (3000r / min) and mix for 5-30 minutes to form a W / O dispersion. The prepared W / O dispersion was electrospun using a single-needle electrospinning device with a voltage of 14kV, a flow rate of the dispersion of 0.4mL / h, and a receiving distance of 18cm for electrospinning. After 12 hours, the collected fibers were electrospun with a diameter of 400nm to 1μm. The composite film has a thickness of 10 μm to 50 μm, and the chitosan gel content is 0.2% to 0.3%. Taking HRP as a drug model, the encapsulation rate is above 70% (mass), and the burst release rate is below 20%, achieving the purpose...

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Abstract

The invention discloses a polylactide electrospinning composite film for in-situ gel encapsulating and a preparation method of the polylactide electrospinning composite film. The electrospinning composite film consists of poly(lactide-co-glycolide) or polyethylene glycol-b-poly(lactide-co-caprolactone) fibers and chitosan or gelatin gel particles in the fibers. The preparation method comprises the steps that: sulfydryl chitosan or sulfydryl gelatin and polyethyleneglycol diacrylate are dissolved in phosphate buffer liquid to be used as water phases; the poly(lactide-co-glycolide) or the polyethylene glycol-b-poly(lactide-co-caprolactone) is dissolved in mixed organic solvents to be used as oil phases; and the water phases are added to the oil phases, water/oil (W/O) dispersing liquid is formed through adding emulsifying agents under the stirring condition, and the electrospinning composite film is obtained through the electrospinning of the dispersing liquid. The polylactide electrospinning composite film and the preparation method have the advantages that the preparation process is simple, the prepared composite film has good biocompatibility, degradability, hydrophily and mechanical property, the medicine dumping is reduced, and the medicine release speed is adjustable and controllable. The polylactide electrospinning composite film and the preparation method are used for the tissue engineering and medicine release fields.

Description

technical field [0001] The invention relates to a polylactide electrospun composite membrane with in-situ gel loading and a preparation method thereof, belonging to tissue engineering and drug controlled release material technology. Background technique [0002] Electrospun microfiber membranes have high specific surface area and porosity, can simulate extracellular matrix, and can be used as tissue engineering scaffolds. In addition, drugs loaded in fibers can also be used as drug release carriers, which have received extensive attention (Sill T J, von Recum H A, Biomaterials, 2008, 29(13):1989-2006). [0003] Biodegradable polylactide copolymers [poly(lactide-co-glycolide) (PLGA) or polyethylene glycol-b-poly(lactide-co-caprolactone) (PELCL)] biophase Good capacitance and excellent mechanical properties. Electrospinning technology is used to prepare ultrafine fiber electrospun membranes from these polymers, which simulate the structure of extracellular matrix and can be ...

Claims

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Application Information

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IPC IPC(8): D04H1/728D01D1/02
Inventor 袁晓燕韩凤选张红赵瑾赵蕴慧
Owner TIANJIN UNIV
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