Unlock instant, AI-driven research and patent intelligence for your innovation.

High performance liquid chromatography for detecting hexachlorophene in cosmetics

A technology for high performance liquid chromatography and cosmetics, which is applied in the field of high performance liquid chromatography for detecting hexachlorophenol in cosmetics, and achieves the effects of rapid detection, good reproducibility and simple sample processing

Inactive Publication Date: 2012-06-20
CHINESE ACAD OF INSPECTION & QUARANTINE +1
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

According to the inquiry of the Standard Hall of the China Standard Research Center, there is currently no standard for the inspection method of hexachlorophene in cosmetics at home and abroad.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • High performance liquid chromatography for detecting hexachlorophene in cosmetics
  • High performance liquid chromatography for detecting hexachlorophene in cosmetics
  • High performance liquid chromatography for detecting hexachlorophene in cosmetics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1. The linear relationship of the method

[0042] Measure the linear relationship under the following conditions: Chromatographic column: Symmetry C18, particle diameter 5 μ m, length 250 mm, diameter 4.6 mm; Mobile phase: acetonitrile-phosphoric acid aqueous solution with a volume ratio of 80:20, wherein the volume percent of phosphoric acid in the phosphoric acid aqueous solution is 0.5 %, isocratic elution; flow rate: 1.0mL / min; column temperature: 20°C; Draw working curve with peak area to mass concentration, hexachlorophene composition is in 0.2×10 -5 ~0.2×10 -2 There is a linear relationship between g / L and their corresponding peak areas, and the correlation coefficient is greater than 0.999, see Table 1. When the hexachlorophene content in the sample exceeds this linear range, the dilution factor of the sample can be increased appropriately.

[0043] 2. Method detection limit

[0044] Under the condition that the signal-to-noise ratio is 10 (S / N=10), the indo...

Embodiment 2

[0061] 1. The linear relationship of the method

[0062]Measure the linear relationship under the following conditions: chromatographic column: Kromasil C18, particle diameter 5 μm, length 250mm, diameter 4.6mm; Mobile phase: volume ratio is the acetonitrile-phosphoric acid aqueous solution of 77:23, wherein the volume percent of phosphoric acid in the phosphoric acid aqueous solution is 0.4 %, isocratic elution; flow rate: 1.0mL / min; column temperature: 20°C; injection volume: 10μL; equipped with a diode array detector, detection wavelength: 200nm; Inject the sample, draw the working curve with the peak area versus the mass concentration, and the hexachlorophene component is at 0.2×10 -5 ~0.2×10 -2 There is a linear relationship between g / L and their corresponding peak areas, and the correlation coefficient is greater than 0.999, as shown in Table 6. When the hexachlorophene content in the sample exceeds this linear range, the dilution factor of the sample can be increased ...

Embodiment 3

[0081] 1. The linear relationship of the method

[0082] Measure the linear relationship under the following conditions: chromatographic column: Discovery C18, particle diameter 5 μm, length 250mm, diameter 4.6mm; Mobile phase: volume ratio is the acetonitrile-phosphoric acid aqueous solution of 82:18, wherein the volume percent of phosphoric acid in phosphoric acid aqueous solution is 0.6 %, isocratic elution; flow rate: 1.0mL / min; column temperature: 40°C; Draw working curve with peak area to mass concentration, hexachlorophene composition is in 0.2×10 -5 ~0.2×10 -2 There is a linear relationship between g / L and their corresponding peak areas, and the correlation coefficient is greater than 0.999, see Table 11. When the hexachlorophene content in the sample exceeds this linear range, the dilution factor of the sample can be increased appropriately.

[0083] 2. Method detection limit

[0084] Under the condition that the signal-to-noise ratio is 10 (S / N=10), the indoor ex...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
melting pointaaaaaaaaaa
particle diameteraaaaaaaaaa
diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a high performance liquid chromatography for detecting hexachlorophene in cosmetics and relates to a test or analysis method for separating compositions into various groups through adsorption. The high performance liquid chromatography has the advantages of processing a sample easily and conveniently, along with high detection speeds, accurate results and high repeatability. The high performance liquid chromatography comprises the following steps of: 1) processing the sample, namely dissolving, uniformly mixing, centrifuging and filtering; and 2) setting the following conditions of a high performance liquid chromatograph and feeding, namely selecting a C18 chromatographic column with the particle size of 5mu m, the length of 250mm and the diameter of 4.6mm in a mobile phase of a mixed system consisting of acetonitrile and a phosphoric acid aqueous solution, and performing gradient elution at the flow velocity of 1.0mL per minute at a chromatographic column temperature of between 20 and 40 DEG C, wherein the volume ratio of the acetonitrile to the phosphoric acid aqueous solution is (77:23)-(82:18), the volume percentage of phosphoric acid in the phosphoric acid aqueous solution is 0.4 to 0.6 percent, and the liquid chromatograph is provided with an ultraviolet detector or a diode array detector with a detection wavelength of 200 to 210nm.

Description

technical field [0001] The present invention relates to a method for testing or analyzing a composition by separating it into individual components by adsorption. Background technique [0002] Hexachlorophene is a white or slightly yellow crystalline powder with a melting point of 164-165°C. Soluble in alcohol, ether, acetone, chloroform, propylene glycol, polyethylene glycol, olive oil, cottonseed oil or dilute lye, practically insoluble in water. Odorless or slightly phenolic. Because one of the hydroxyl groups reacts with alkali to form phenoxide under alkalinity, and the other hydroxyl group has a bactericidal effect and has special effects on Gram-positive bacteria, it is often added to soaps, creams, baby powders, shampoos, shampoos, etc. Hair shampoo, hair tonic and other cosmetics for daily use and medical products. [0003] According to the provisions of my country's "Hygienic Standards for Cosmetics", hexachlorophene is a prohibited substance in cosmetics. Acco...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 马强王超吴森明杨天宇张丽娟白桦苏宁
Owner CHINESE ACAD OF INSPECTION & QUARANTINE