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Composite microbial additive for feed and preparation method of composite microbial additive

A technology of compound microorganisms and additives, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve problems such as antibiotic residues

Inactive Publication Date: 2012-07-04
HARBIN YINGRUISI FEED
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the use of antibiotics as feed additives in the past has brought huge economic benefits to the feed industry, the side effects such as antibiotic residues have attracted more and more attention.

Method used

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  • Composite microbial additive for feed and preparation method of composite microbial additive
  • Composite microbial additive for feed and preparation method of composite microbial additive
  • Composite microbial additive for feed and preparation method of composite microbial additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The assay method of embodiment 1 viable count

[0025] The determination of the number of live bacillus bigenous bacteria is counted by the plate dilution coating method, and the method is carried out according to the following steps:

[0026] 1) The culture medium was prepared according to the following formula: 1% peptone, 0.5% yeast extract powder, 1% NaCl, 1% agar, natural pH, and sterilized at 121° C. for 15-20 minutes.

[0027] 2) After the medium in step 1) is sterilized, the temperature drops to 40-50°C, pour it into a sterilized petri dish, the dosage is 15-25mL / plate, and let it stand for a while until the medium solidifies.

[0028] 3) After diluting the sample to be tested (fermentation broth or bacterial powder) to a certain concentration, take 100 μL (or 200 μL) of the bacterial solution and add it to the medium in step 2).

[0029] 4) After uniformly coating the bacterial solution in step 3) with a coating rod, invert the culture dish for 24 hours and th...

Embodiment 2

[0030] The assay method of embodiment 2 sporulation rate

[0031] Take 1 mL of cultured fermented liquid (or bacteria powder) and add it to 9 mL of sterilized physiological saline, treat it in a water bath at 80°C for 10 minutes, immediately cool it to room temperature with water, and measure the number of viable bacteria according to the method described in Example 1, which is the spore number.

[0032]

Embodiment 3

[0033] The mensuration of viable count and spore rate in the compound microbial additive preparation process of embodiment 3 of the present invention

[0034] Actual production data in the fermentation process of the present invention is shown in Table 1, and the composite microecological preparation obtained by fermentation has very high thalline activity by the known fermentation of Table 1, and the number of live bacteria in fermented liquid reaches 10 10 More than CFU / g, the spore rate reaches 77.3%; the bacterial activity of the compound microbial additive is as high as 8.7×10 10CFU / g, spore rate reaches 96.1%. It can be seen that the compound microbial feed additive of the present invention not only has a high number of viable bacteria, but also has a high rate of spore formation, which also indicates that the compound microbial feed additive of the present invention has certain stress resistance and can resist feed system. Unfavorable environment for heat and animal st...

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Abstract

The invention discloses a composite microbial additive for feed, in particular to a microbial feed additive containing two kinds of bacilli and a preparation method of the microbial feed additive, and belongs to the technical field of microbial feed additives. According to the microbial feed additive, bacillus subtilis and bacillus licheniformis are mixed according to a certain proportion for fermentation culture, and after bacterium mud obtained through fermentation liquid centrifugation is treated, the low-temperature baking is carried out to obtain powder agents. The microbial feed additive prepared by the method has the advantages that the viable bacterium content is high, the heat resistance and the animal intestine and stomach environment resistance are realized, the storage period of the product is long, the effects of balancing the animal intestinal tract flora and improving the immunity and the disease resistance can be realized after the microbial feed additive is added into animal feed, the weight gain is promoted, the feed conversion rate is improved, the material-weight ratio is reduced, and the microbial feed additive has various effects of treatment, prevention, health care and immunity enhancement.

Description

technical field [0001] The invention discloses a compound microbial feed additive for feed, in particular to a compound microbial feed additive containing two bacillus species, Bacillus subtilis K2008 and Bacillus licheniformis DY01, and a preparation method thereof. The invention belongs to the technical field of microbial feed additives. Background technique [0002] As people pay more and more attention to their own health and food safety, the importance of safe and green feed additives has become increasingly prominent. Although the use of antibiotics as feed additives in the past has brought huge economic benefits to the feed industry, the side effects such as antibiotic residues have attracted more and more attention. With the advantages of multiple functions, no toxic side effects, no residue, and no pollution to the environment, probiotics have attracted widespread attention from domestic and foreign scholars and the feed industry as feed additives instead of antibi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K1/16C12N1/20C12R1/125C12R1/10
Inventor 赵敏鹿晓菲
Owner HARBIN YINGRUISI FEED
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