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Method for preparing tea saponin/copper compound antibacterial agent

A compound antibacterial agent and tea saponin technology, applied in the biochemical field of renewable resources, can solve the problems of drug resistance, antibacterial delayed effect, high price, etc., and achieve no drug resistance reaction conditions, easy operation, and low price cheap effect

Active Publication Date: 2012-07-18
FUJIAN SHENGHUA AGRI SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Organic antibacterial agents have the advantages of strong bactericidal power and fast speed, but they have disadvantages such as poor safety and easy drug resistance; while inorganic antibacterial agents have broad spectrum, no drug resistance and antibacterial failure, but they are expensive. And has the defects of antibacterial delayed effect

Method used

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  • Method for preparing tea saponin/copper compound antibacterial agent
  • Method for preparing tea saponin/copper compound antibacterial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] The tea saponin that 100g purity is 85% is dissolved in distilled water, forms the tea saponin solution that mass concentration is 10%, adds 55g cupric chloride solid under the condition of stirring, after stirring it to dissolve, continue to stir 3 hours, Then dry by a spray drier to obtain a powdered tea saponin / copper composite antibacterial agent.

[0020] The tea saponin / copper composite antibacterial agent prepared in this example was compared with a single tea saponin (comparative example 1) and copper chloride (comparative example 2). The test results are shown in Table 1.

[0021] Table 1 Minimum Inhibitory Concentration Unit: mg / ml

[0022] microorganism Comparative example 1 Comparative example 2 This example Escherichia coli ATCC29252 6 15.5 0.5 Staphylococcus aureus 15 17.5 0.75 Salmonella enterica 6 18 1

Embodiment 2

[0024] 100g of tea saponin with a purity of 72% is dissolved in distilled water to form a mass concentration of 10% tea saponin solution, add 100g of copper sulfate solid, 35g of cupric chloride solid and 30g of copper nitrate solid under stirring, and stir After making it dissolve, continue to stir for 3 hours, then dry by a spray drier to obtain a powdered tea saponin / copper composite antibacterial agent.

[0025] The tea saponin / copper composite antibacterial agent prepared in this example was compared with a single tea saponin (comparative example 1) and copper chloride (comparative example 2). The test results are shown in Table 2.

[0026] Table 2 Minimum inhibitory concentration unit: mg / ml

[0027] microorganism Comparative example 1 Comparative example 2 This example Escherichia coli ATCC29252 6 15.5 1 Staphylococcus aureus 15 17.5 0.75 Salmonella enterica 6 18 1.5

Embodiment 3

[0029] 100g purity is that the tea saponin of 90% is dissolved in distilled water, forms the tea saponin solution that mass concentration is 10%, adds 70g cupric chloride solid and 30g cupric nitrate solid under the condition of stirring, after stirring it to dissolve, Continue to stir for 4 hours, then dry by a spray dryer to obtain powdered tea saponin / copper composite antibacterial agent.

[0030] The tea saponin / copper composite antibacterial agent prepared in this example was compared with a single tea saponin (comparative example 1) and copper chloride (comparative example 2). The test results are shown in Table 3.

[0031] Table 3 Minimum Inhibitory Concentration Unit: mg / ml

[0032] microorganism Comparative example 1 Comparative example 2 This example Escherichia coli ATCC29252 6 15.5 0.5 Staphylococcus aureus 15 17.5 0.75 Salmonella enterica 6 18 1

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Abstract

The invention discloses a method for preparing a tea saponin / copper compound antibacterial agent, and the preparing steps include:adding natural product tea saponin and copper ions by mass ratio of (0.1-2) : 1 with stirring, keeping stirring for 2 hours to 6 hours, drying by a spray drier and then obtaining the tea saponin / copper compound antibacterial agent. The purity of the tea saponin is above 70%. The copper ions are a mixture prepared from one or more than two of solid copper sulfate, cupric nitrate and copper chloride in an arbitrary proportion. The compound antibacterial agent has an excellent broad-spectrum antibacterial property and obvious inhibiting effect for various pathogenic bacteria such as Escherichia coil, staphylococcus aureus and the like. Compared with the bacteriostatic activity of using tea saponin or a metal new antibacterial agent alone, the bacteriostatic activity of the compound antibacterial agent is remarkably enhanced, and the compound antibacterial agent is widely used in the fields of daily chemicals, textile and food and the like, and has a good harmony and a high security with the environment. According to the method for preparing the tea saponin / copper compound antibacterial agent, the antibacterial agent has the advantages of simple technology, low cost, easiness in industrialization and stable quality and the like.

Description

technical field [0001] The invention relates to a method for preparing a novel composite antibacterial agent by utilizing natural renewable resources tea saponin and copper ions to coordinate, and belongs to the field of biochemistry of renewable resources. Background technique [0002] Organic antibacterial agents have the advantages of strong bactericidal power and fast speed, but they have disadvantages such as poor safety and easy drug resistance; while inorganic antibacterial agents have broad spectrum, no drug resistance and antibacterial failure, but they are expensive. And there are defects such as antibacterial delayed effect. [0003] Tea saponin is a sugar compound extracted from the seeds of the Theaceae plant. The molecule has a hydrophilic sugar body and a hydrophobic coordination group. It belongs to triterpenoid saponins. It is composed of sugar body, ligand and organic acid composition. It has emulsifying, dispersing, moisturizing, decontaminating, foaming...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N59/20A01N43/16A01P1/00
Inventor 卢玉栋吴宗华游瑞云翁景峥沈慧英叶清萍李璇
Owner FUJIAN SHENGHUA AGRI SCI & TECH DEV
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