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Microbial inoculant for improving disease resistance and promoting growth of plants

A technology of microbial agent and microbial strain, applied in the direction of plant growth regulators, plant growth regulators, microorganisms, etc., can solve the problems of soil microbial zone interference, enhanced drug resistance of pathogenic bacteria, difficulty in functioning normally, etc., to promote growth , growth enhancement, good market prospects

Inactive Publication Date: 2012-07-18
TIANJIN CITY AGRI BIO TECH RES CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the protected area is an ecological environment with high humidity and low light, so the disease resistance of cucumbers is reduced, and diseases occur frequently, including soil-borne fungal diseases such as damping-off and fusarium wilt; air-borne fungi such as gray mold and powdery mildew diseases; and diseases caused by other bacteria and viruses
At present, chemical control methods are mainly used in production to control the occurrence and development of these diseases, but due to the biological characteristics of soil-borne disease pathogens and the barrier function of the soil, the control difficulty is increased, resulting in the use of large amounts of chemical control agents, but it is difficult to work normally. , the soil microbial flora is disturbed, the drug resistance of pathogenic bacteria is enhanced, and the soil and cucumber are seriously polluted

Method used

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  • Microbial inoculant for improving disease resistance and promoting growth of plants
  • Microbial inoculant for improving disease resistance and promoting growth of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Preparation of bacteria (Bacillus subtilis)

[0033] (1) Bacillus subtilis Isolation of EBC7: Collect healthy “Jinyou 10” cucumber roots from the greenhouse in Xiqing District, Tianjin; rinse with running water for 1 hour, soak in 70% ethanol for 1 minute, and disinfect in 2.5% NaClO for 6 minutes , rinse with sterile water and set aside;

[0034](2) Transfer the sterilized tissue obtained in step (1) to a sterile mortar and add PBS buffer solution for grinding, take the grinding juice and serially dilute, apply 100 μl each on the NA plate, and place it at 28°C Dark culture, each treatment repeated 3 times;

[0035] (3) Pick a single colony on the NA plate, streak and purify it, and obtain the Bacillus subtilis strain; then inoculate the Bacillus subtilis in the NB culture medium, shake it at 150 rpm / min for 24 hours, and then inoculate the obtained Bacillus subtilis Store the bacillus solution in 15% glycerol at -80°C for later use; NA medium: 10g peptone, 5g NaCl,...

Embodiment 2

[0037] Bacteria (Bacillus subtilis) are stored in 15% glycerol at -80°C, and inoculated on NA solid medium for activation and culture when in use; after activation, take a ring of bacterial lawn and inoculate it in a triangular flask containing NB medium for seed culture. After shaking at 150 rpm / min for 24 h, inoculate 3% of the inoculum in a fermentation bottle and ferment for 48 h to obtain a content of 1.5×10 10 The fermentation broth contains vegetative bodies and / or spores of Bacillus and antibacterial metabolites.

[0038] NA medium: peptone 10g, NaCl 5g, beef extract 3g, agar powder 12g, water up to 1000ml, pH7.0;

[0039] NB medium (seed culture medium): peptone 10g, NaCl 5g, beef extract 3g, water up to 1000ml, pH7.0;

[0040] Fermentation medium: soybean powder 10.00g, corn starch 5.00g, yeast powder 0.50g, KH 2 PO 4 0.35g, CaCl 2 0.20g, add water to 1000ml, pH7.0.

Embodiment 3

[0042] Bacteria (Bacillus subtilis) are stored in 15% glycerol at -80°C, and inoculated on NA solid medium for activation and culture when in use; after activation, take a ring of bacterial lawn and inoculate it in a triangular flask containing NB medium for seed culture. After shaking at 150 rpm / min for 24 h, inoculate 3% of the inoculum in the fermentation bottle and ferment for 48 h to obtain a content of 4.5×10 10 Fermentation broth (containing vegetative and / or spores of Bacillus and antibacterial metabolites).

[0043] NA medium: peptone 10g, NaCl 5g, beef extract 3g, agar powder 12g, water up to 1000ml, pH7.0;

[0044] NB medium: peptone 10g, NaCl 5g, beef extract 3g, water up to 1000ml, 7.2;

[0045] Fermentation medium: soybean powder 10.00g, corn starch 5.00g, yeast powder 0.50g, KH 2 PO 4 0.35g, CaCl 2 0.20g, add water to 1000ml, pH7.2.

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Abstract

The invention discloses a microbial inoculant for improving disease resistance and promoting growth of plants, and discloses a microbial inoculant for improving disease resistance and promoting growth of cucumbers. The main active ingredient of the microbial inoculant is EBC7 fermentation broth with Bacillussubtilis content of 1.5-4.5*10<10>. The invention also discloses application of the microbial inoculant in the aspects of improving the disease resistance and promoting the growth of the cucumbers. The relative control effect on cucumber fusarium wilt is 78.5 percent; the relative control effect on powdery mildew of cucumber is 80.4 percent; and moreover, the plant height of the cucumbers and fresh and dry weight of overground parts are obviously increased. The bacillus subtilis preparation can be widely applied to agricultural production, safe without pollution, and can restore and keep the ecological balance of soil environment.

Description

technical field [0001] The present invention relates to a kind of microbial agent, more specifically a kind of Bacillus subtilis ( Bacillus subtilis ) The microbial agent of EBC7 live bacteria or fermented bacterial liquid and the application of the agent in promoting the growth of cucumber and improving its disease resistance. Background technique [0002] Cucumber has been cultivated in my country for more than 2,000 years. It is rich in vitamins A, C and various beneficial minerals. However, the protected area is an ecological environment with high humidity and low light, so the disease resistance of cucumbers is reduced, and diseases occur frequently, including soil-borne fungal diseases such as damping-off and fusarium wilt; air-borne fungi such as gray mold and powdery mildew diseases; and diseases caused by other bacteria and viruses. At present, chemical control methods are mainly used in production to control the occurrence and development of these diseases, but d...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P21/00C12R1/125
Inventor 岳东霞张要武于海涛
Owner TIANJIN CITY AGRI BIO TECH RES CENT