Metribuzin-degrading bacteria and soil bioremediation microorganism based on metribuzin-degrading bacteria and application
A technology of soil biology and restoration of bacterial agents, applied in the biological field, can solve the problems of sensitive crops that are prone to phytotoxicity, affect the growth of higher plants, and have a long residual time, so as to protect the ecological environment and food safety, eliminate pesticide residues, and be easy to use Effect
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Embodiment 1
[0026] Example 1: Enrichment, separation, purification and preparation of bacterial suspension of mecitrione-degrading bacteria
[0027] Get 5g of soil samples (Jilin Agricultural University Agricultural Science Experimental Station has used metrimetrione test field for many years) and add it into a triangular flask filled with 100mL inorganic salt medium, and take 2mL from the prepared metrimetrione solution with a concentration of 1000mg / L. Put it into the Erlenmeyer flask as the only nitrogen source (20mg / L) for microbial growth, culture on a shaker for 2d (30°C, 160r / min) and then transfer to the inorganic salt medium containing mecitrione 50mg / L for 2d. , and then insert 0.1 mL of the above-mentioned inorganic salt culture medium for 2 days on the plate culture medium containing metrimetrione with a concentration of 100 mg / L, and culture it in a 30°C incubator for 2 days after coating, and pick out a single colony on the plate Repeated transplantation and streaking for 3 ...
Embodiment 2
[0030] Embodiment 2: degradation condition
[0031] 1. The effect of pH on the degradation of mecitrione by strain N1
[0032] According to the percentage by volume, the inoculation amount is 5%, and the N1 bacterial suspension prepared in Example 1 is inserted into the inorganic salt medium whose concentration of mecitrione is 20mg / L, and the pH value of the medium is adjusted to 5.0, 6.0, 6.5 respectively. , 7, 7.5, 8.0, 9.0, cultured on a shaking table at 30°C and 160rpm, and measured the content of metrizone at 24, 48, 72, 96, and 120 hours respectively.
[0033] The formula of the inorganic salt culture medium is by weight percentage: sucrose 3g, KH 2 PO 4 0.5g, MgSO 4 ·7H 2 O 0.4g, K 2 HPO 4 1.5g, 1g NaCl, add 1000mL distilled water, the pH is natural.
[0034] The experimental results of the pH effect on the degradation of metrizone by the strain showed that when the pH was 7.0, the degradation rate reached 78.43% within 120 hours, and the degradation efficiency ...
Embodiment 3
[0050] Embodiment 3: Bacteria agent preparation test
[0051] Use an inoculation loop to inoculate a little bacteria on the plate culture medium in the slant test tube strain of mecitrione-degrading bacteria N1, incubate at 30°C for 48 hours, then use an inoculation loop to inoculate a little bacteria in LB medium, 30°C Shake culture to logarithmic phase.
[0052] The formula of described plate culture medium is by weight percentage: NaCl 5g, peptone 10g, beef extract 5g, agar 15g, NaHPO 4 1.50g, add 1000mL distilled water; the LB medium is: NaCl 10g, yeast powder 5g, tryptone 5g, add 1000mL distilled water, the pH is 7.0; put 10mL of the cultured strain into the seed bottle, shake and cultivate to logarithmic period; 1000mL seed bottle medium is: sucrose 5g, MgSO 4 ·7H 2 O 0.8g, K 2 HPO 4 2g, NaCl 1g, KH 2 PO 4 0.5g, 0.2g yeast extract, add 1000mL water, the pH of the medium is 7.0;
[0053] Add 15 mL of the above-mentioned cultured strains to 1000 g of fermentatio...
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