Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Preparation method of Fe3O4 nano-magnetic resonance contrast medium material

A magnetic resonance contrast agent, nanotechnology, applied in the direction of nuclear magnetic resonance/magnetic resonance imaging contrast agent, etc., to achieve the effects of good biocompatibility, short response time and low price

Active Publication Date: 2013-11-27
SHANGHAI NORMAL UNIVERSITY
View PDF6 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at this stage, the research on this multifunctional and biocompatible nanomaterial is a current problem and difficulty, and further development and research are still needed.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of Fe3O4 nano-magnetic resonance contrast medium material
  • Preparation method of Fe3O4 nano-magnetic resonance contrast medium material
  • Preparation method of Fe3O4 nano-magnetic resonance contrast medium material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Weigh sodium citrate (0.2g, 0.68mmol) and dissolve it in 20ml ethylene glycol, add anhydrous FeCl after ultrasonic stirring to dissolve 3 (0.3244g, 2.0mmol), after ultrasonic dissolution, add 1.20g CH 3 COONa·3H 2 O (9mmol), dissolved by ultrasound, stirred for 30min, transferred the solution to a 50ml reactor, and reacted at 200°C for 10h. After cooling, the precipitate was washed three times with ethanol and deionized water, and freeze-dried to obtain superparamagnetic Fe 3 o 4 Magnetic nanoparticles, water soluble. The particle size is 200-300nm, as shown in the transmission electron microscope figure 1 As shown, the XRD spectrum is as figure 2 shown.

Embodiment 2

[0041] Prepare the NaOH solution of 10ml 5mol / L, and transfer in the 100ml single-necked round bottom flask, add the dry good Fe in 60mg embodiment 1 3 o 4 Magnetic nanoparticles, stirred for 2h, then added dropwise 6ml epichlorohydrin (55mmol) under the protection of nitrogen, stirred for 24h under the protection of nitrogen, the precipitate was taken and washed three times with deionized water. After washing, redissolve the particles in 25ml of deionized water, add 12ml of ammonia water (concentration: 25wt%-28wt%, about 0.2mol), and stir at 37°C for 36h. Washed three times with ethanol and deionized water respectively, and freeze-dried to obtain amino-functionalized superparamagnetic Fe 3 o 4 Nanoparticle Fe 3 o 4 NH 2 .

Embodiment 3

[0043] Folic acid (FA) (2.5mg, 1.08×10 -5 mol) was dissolved in 20ml dimethyl sulfoxide (DMSO), then 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) and N-hydroxysuccinimide were added Amine (NHS), FA, EDC and NHS were input according to the feed ratio (molar ratio) of 1:1:2.5, and stirred for 3h under the protection of nitrogen. Then the amino functionalized dry good Fe in embodiment 2 3 o 4 Weigh 40mg and dissolve it in 10ml DMSO, then add it dropwise to the above solution, stir overnight under nitrogen protection, finally wash with phosphate buffer saline (PBS) three times, and freeze-dry to obtain the superciliary substance attached to the target substance. Magnetic Fe 3 o 4 Nano-magnetic resonance contrast agent material Fe 3 o 4 NH 2 Fa.

[0044] The Fe obtained by the above method 3 o 4 Nano-magnetic resonance contrast agent material, the shape is spherical, the particle size is 200-300nm, and Fe with a particle size of 200-300nm 3 o 4 M...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Fe3O4 nano-magnetic resonance contrast medium material and a preparation method thereof, water is used as dissolvent, the catalyst NaOH is added, epichlorohydrin is used as an open-loop raw material, the open-loop polymerization reaction is carried out between epichlorohydrin and hydroxyl on the surface of the Fe3O4 nano granule, the branch-shaped polymer of the functionalized active function group is modified in a normal position of the nano granule surface, an amidogen group is connected, targeting molecular folvite is coupled to enable the granule has a targeting function, the contrast medium material can be applied in the targeting magnetic resonance imaging of a disease. The Fe3O4 nano-magnetic resonance contrast medium material is in a ball shape, has the granule diameter of 200-300nm, and has good dispersion, water-solubility, stability and biological compatibility, high saturated susceptibility, and strong capability on relaxation.

Description

technical field [0001] The invention relates to the fields of materials and medical biology, discloses a nano-magnetic resonance contrast agent material and a preparation method thereof, in particular to superparamagnetic Fe 3 o 4 Surface modification and functionalization of nanoparticles. Background technique [0002] Fe 3 o 4 Nanoparticles can be used as magnetic resonance contrast agents due to their superparamagnetic properties. The distribution of superparamagnetic contrast agents in the human body has the advantages of specificity, low dosage, safety, small toxic and side effects, and wide application. At present, the preparation methods of magnetic nanoparticles include liquid-phase synthesis of nanoparticles, which has the characteristics of simple equipment, easy-to-obtain raw materials, controllable composition, high purity, and uniform particle size. Fe 3 o 4 Nanoparticles are generally synthesized by pyrolysis, co-precipitation and hydrothermal methods, a...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/06
Inventor 杨仕平黄国胜胡鹤代安涛安璐张崇琨
Owner SHANGHAI NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com