Preparation method of Fe3O4 nano-magnetic resonance contrast medium material

A magnetic resonance contrast agent, nanotechnology, applied in the direction of nuclear magnetic resonance/magnetic resonance imaging contrast agent, etc., to achieve the effects of good biocompatibility, short response time and low price

Active Publication Date: 2013-11-27
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at this stage, the research on this multifunctional and biocompatible nanomaterial is a current problem and difficulty, and further development and research are still needed.

Method used

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  • Preparation method of Fe3O4 nano-magnetic resonance contrast medium material
  • Preparation method of Fe3O4 nano-magnetic resonance contrast medium material
  • Preparation method of Fe3O4 nano-magnetic resonance contrast medium material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Weigh sodium citrate (0.2g, 0.68mmol) and dissolve it in 20ml ethylene glycol, add anhydrous FeCl after ultrasonic stirring to dissolve 3 (0.3244g, 2.0mmol), after ultrasonic dissolution, add 1.20g CH 3 COONa·3H 2 O (9mmol), dissolved by ultrasound, stirred for 30min, transferred the solution to a 50ml reactor, and reacted at 200°C for 10h. After cooling, the precipitate was washed three times with ethanol and deionized water, and freeze-dried to obtain superparamagnetic Fe 3 o 4 Magnetic nanoparticles, water soluble. The particle size is 200-300nm, as shown in the transmission electron microscope figure 1 As shown, the XRD spectrum is as figure 2 shown.

Embodiment 2

[0041] Prepare the NaOH solution of 10ml 5mol / L, and transfer in the 100ml single-necked round bottom flask, add the dry good Fe in 60mg embodiment 1 3 o 4 Magnetic nanoparticles, stirred for 2h, then added dropwise 6ml epichlorohydrin (55mmol) under the protection of nitrogen, stirred for 24h under the protection of nitrogen, the precipitate was taken and washed three times with deionized water. After washing, redissolve the particles in 25ml of deionized water, add 12ml of ammonia water (concentration: 25wt%-28wt%, about 0.2mol), and stir at 37°C for 36h. Washed three times with ethanol and deionized water respectively, and freeze-dried to obtain amino-functionalized superparamagnetic Fe 3 o 4 Nanoparticle Fe 3 o 4 NH 2 .

Embodiment 3

[0043] Folic acid (FA) (2.5mg, 1.08×10 -5 mol) was dissolved in 20ml dimethyl sulfoxide (DMSO), then 1-ethyl-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC·HCl) and N-hydroxysuccinimide were added Amine (NHS), FA, EDC and NHS were input according to the feed ratio (molar ratio) of 1:1:2.5, and stirred for 3h under the protection of nitrogen. Then the amino functionalized dry good Fe in embodiment 2 3 o 4 Weigh 40mg and dissolve it in 10ml DMSO, then add it dropwise to the above solution, stir overnight under nitrogen protection, finally wash with phosphate buffer saline (PBS) three times, and freeze-dry to obtain the superciliary substance attached to the target substance. Magnetic Fe 3 o 4 Nano-magnetic resonance contrast agent material Fe 3 o 4 NH 2 Fa.

[0044] The Fe obtained by the above method 3 o 4 Nano-magnetic resonance contrast agent material, the shape is spherical, the particle size is 200-300nm, and Fe with a particle size of 200-300nm 3 o 4 M...

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Abstract

The invention discloses a Fe3O4 nano-magnetic resonance contrast medium material and a preparation method thereof, water is used as dissolvent, the catalyst NaOH is added, epichlorohydrin is used as an open-loop raw material, the open-loop polymerization reaction is carried out between epichlorohydrin and hydroxyl on the surface of the Fe3O4 nano granule, the branch-shaped polymer of the functionalized active function group is modified in a normal position of the nano granule surface, an amidogen group is connected, targeting molecular folvite is coupled to enable the granule has a targeting function, the contrast medium material can be applied in the targeting magnetic resonance imaging of a disease. The Fe3O4 nano-magnetic resonance contrast medium material is in a ball shape, has the granule diameter of 200-300nm, and has good dispersion, water-solubility, stability and biological compatibility, high saturated susceptibility, and strong capability on relaxation.

Description

technical field [0001] The invention relates to the fields of materials and medical biology, discloses a nano-magnetic resonance contrast agent material and a preparation method thereof, in particular to superparamagnetic Fe 3 o 4 Surface modification and functionalization of nanoparticles. Background technique [0002] Fe 3 o 4 Nanoparticles can be used as magnetic resonance contrast agents due to their superparamagnetic properties. The distribution of superparamagnetic contrast agents in the human body has the advantages of specificity, low dosage, safety, small toxic and side effects, and wide application. At present, the preparation methods of magnetic nanoparticles include liquid-phase synthesis of nanoparticles, which has the characteristics of simple equipment, easy-to-obtain raw materials, controllable composition, high purity, and uniform particle size. Fe 3 o 4 Nanoparticles are generally synthesized by pyrolysis, co-precipitation and hydrothermal methods, a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/06
Inventor 杨仕平黄国胜胡鹤代安涛安璐张崇琨
Owner SHANGHAI NORMAL UNIVERSITY
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