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Pteris multifida extract, preparation method thereof and use thereof

The technology of an extract and Pteris vulgaris, which is applied in the field of preparation of the extract, can solve the problems of being unsuitable for industrial production, difficult to obtain stable composition, good process controllability, etc., to achieve easy industrial production, reduce purification cost, and protect the environment. non-polluting effect

Inactive Publication Date: 2012-09-26
苏州世林医药技术发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the existing macroporous resin preparation technology of the total flavonoids of Pteris vulgaris, the purity of the total flavonoids in the optimized process is 51.8%, and the organic solvent degreasing treatment is generally used, but this method is not suitable for industrial production.
On the other hand, the existing technology is not sure about the composition of total flavonoids in the product, so it is difficult to obtain products with stable composition and good process controllability in actual production

Method used

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  • Pteris multifida extract, preparation method thereof and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Embodiment 1: the preparation of Pteris vulgaris extract

[0018] The research group conducted a systematic study on the macroporous resin refining process of the total flavonoids of Pteris flavonoids. The research content included static adsorption method and dynamic adsorption method to evaluate and screen different resin types, and carried out adsorption isotherm, saturation The optimization of adsorption leakage curve, sample concentration, gradient elution conditions, flow rate, and column diameter-to-height ratio was carried out to obtain effective fractions of total flavonoids from Pteris chinensis. The main process is as follows:

[0019] 20kg of Pteris vulgaris, extract twice with 70% ethanol under reflux, each time for 1.5 hours, combine the extracts, concentrate under reduced pressure to 20L, add 20L water to stand overnight, filter, and load the solution on the processed AB-8 In the resin column, gradient elution was performed with water, 20% ethanol, 70% a...

experiment example 1

[0023] Experimental example 1: Anti-inflammatory effect test of Pteris vulgaris extract

[0024] Take 40 mice of 20-25g, and divide them into four groups: Pteris vulgaris ethanol extract (12g crude drug / kg), Pteris vulgaris extract (300mg / kg), control group and model group, and they are pre-filled with drugs for one week. Two hours after the last dosing, apply 25 microliters of xylene evenly to the right ear of the mouse, kill the mouse half an hour later, use a 6mm diameter puncher, take the same parts of the left and right ears of the mouse, and weigh them with an analytical balance . The weight of the removed right ear minus the weight of the left ear was the degree of swelling, the mean and standard deviation of the control group and the treatment group were calculated, and the t test was used to compare the significance of the differences between the groups. Calculate the swelling inhibition rate according to the following formula:

[0025] Swelling inhibition rate (%)=...

experiment example 2

[0027] Experimental example 2: Anti-prostatitis effect test of Pteris vulgaris extract

[0028] Select 90 male mice weighing 20-25g, and randomly divide them into normal group, model group, Pteris vulgaris alcohol extract group (12g crude drug / kg), Pteris vulgaris extract group (300mg / kg, 150mg / kg), There were 6 groups in the normal saline control group, the normal control group was subcutaneously injected with ethyl oleate 5ml / kg / d, all the other mice were intraperitoneally injected with 0.1ml of DPT vaccine, and intradermally injected with rat prostatic protein purified solution and complete Ephrodisiac. Adjuvant (ratio of 1:1 suspension) 0.4ml, 0, 30d were injected twice. After 30 days of continuous administration, the mice in each group were sacrificed, and the pathological changes of the prostate were examined.

[0029] Results: 30 days after modeling, the mice had different degrees of pathological manifestations of chronic inflammation; The infiltrated lymphocytes and ...

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Abstract

The invention discloses a pteris multifida extract which is extracted from pteris plants. The pteris multifida extract mainly contains flavonoid ingredients including apigenin-7-O-beta-D-glucose-4'-O-alpha-L-rhamnoside, luteolin-7-O-beta-D-glucoside and the like. The invention also discloses a preparation method of the pteris multifida extract, mainly characterized in that the extract is obtained through processes of ethanol extraction, water precipitation treatment, macroporous resin column purification and the like performed on the pteris plants, wherein the macroporous resin preferably is macroporous resin of weak polarity. The invention further discloses uses of the pteris multifida extract in the fields of medicine and food. The pteris multifida extract is mainly characterized by anti-inflammatory effect, especially anti-prostatitis effect or benign prostatic hyperplasia inhibition effect.

Description

technical field [0001] The present invention relates to a fern extract, and the present invention also relates to the preparation method and application of the extract. Background technique [0002] Pteris multifida Poir. (Pteris multifida Poir.) is a plant of the genus Pteris in the family Pteridaceae. Widely distributed in temperate regions of the world. Traditional Chinese medicine believes that Pteris vulgaris is cold in nature and slightly bitter, and has the effects of reducing swelling and detoxification, clearing away heat and dampness, cooling blood and stopping bleeding. , hematemesis, epistaxis, hematuria, hematochezia and traumatic bleeding embolism, clinically used in the treatment of infectious hepatitis, acute bacillary dysentery and other diseases. According to modern chemical research, its main components are flavonoids, fern-like sesquiterpenes, and kaurane-type diterpenes. -glucoside, apigenin-3′-O-β-D-glucoside, apigenin-7-O-β-D-glucose-4′-O-α-L-rhamno...

Claims

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Application Information

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IPC IPC(8): A61K36/11A23L1/29A61P13/08A61P29/00A23L33/00
Inventor 薛波新刘江云单玉喜向贵王益刘艳丽刘志远王蓉杨世林
Owner 苏州世林医药技术发展有限公司
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