Formula and preparation process of medium for culturing medical shiraia bambusicola henn

A culture medium formula, technology of medicinal fungi, applied in the direction of application, fertilizer mixture, fertilization device, etc., can solve the problems of bacterial strain resource decline, species extinction, etc., and achieve the effects of simple production, improved yield and quality, and good cultivation effect

Inactive Publication Date: 2012-09-26
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, with the increasing development of human society, the urbanization of rural areas, the reclamation and utilization of barren hills, and the destruction of the environment, the host plants of bamboo yellow fungus are becoming less and less, which leads to the decline of bacterial species resources year by year, facing the crisis of species extinction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Put 147kg of mineral water into the 300L reactor, then put in 16.2kg of crushed bamboo leaf juice, 16.2kg of fresh corn juice, 16.2kg of apple juice, 16.2kg of purple potato juice and 16.2kg of poria cocos juice, and then put in 3kg of caramel, 10kg of brown sugar, 10kg of mannose and 10kg of rhamnose, and then add 3kg of Lactobacillus, 3kg of Lactobacillus, 3kg of Bacillus cereus, 3kg of Streptococcus intermedia and 3kg of Lactobacillus rhamnosus, stir well, and then slowly Add 4kg of dipotassium hydrogen phosphate, 4kg of ferrous sulfate, 4kg of ammonium nitrate, 4kg of ammonium sulfate, 4kg of copper sulfate and 4kg of sodium molybdate, and finally stir evenly until all the substances are completely dissolved, and sterilize at 128°C for 30 minutes to complete the final culture base.

Embodiment 2

[0022] Put 96kg of mineral water into the 300L reactor, then put in 18.6kg of crushed bamboo leaf juice, 18.6kg of fresh corn juice, 18.6kg of apple juice, 18.6kg of purple sweet potato juice and 18.6kg of poria cocos juice, then put in 5kg of fructose, 5kg maltose, 5kg galactose, 5kg lactose, 5kg xylose, 10kg glucose, 8kg sucrose and 2kg rhamnose, then add 7kg yeast, 5kg Bacillus subtilis, 5kg Bifidobacterium, 2kg Lactobacillus, 2kg Lamans Lactobacillus , 2kg Bacillus cereus, 2kg Streptococcus intermedius and 2kg Lactobacillus rhamnosus, fully stirred evenly, then slowly put in 9kg Potassium Nitrate, 6kg Sodium Nitrate, 6kg Magnesium Sulfate, 6kg Dipotassium Hydrogen Phosphate, 6kg Ferrous Sulfate and 6kg of sodium molybdate, and finally stir evenly until all the substances are completely dissolved, and sterilize at 129°C for 32min to become the final culture medium.

Embodiment 3

[0024] Put 117kg of mineral water into the 300L reactor, then put in 18kg of crushed and squeezed bamboo leaf juice, 18kg of fresh corn juice, 18kg of apple juice, 18kg of purple potato juice and 18kg of Poria cocos juice, and then put in 2kg of maltose, 4kg of brown sugar, and 4kg of manna Sugar, 4kg fructose, 4kg maltose, 4kg galactose, 4kg lactose, 4kg xylose, 4kg glucose, 4kg sucrose and 4kg rhamnose, and then put in 5kg yeast, 5kg Bacillus subtilis, 5kg bifidobacterium, 5kg intermediary streptococcus and 5kg Lactobacillus rhamnosus, stir well, then slowly add 6kg potassium nitrate, 4kg sodium nitrate, 4kg magnesium sulfate, 4kg ammonium sulfate, 4kg copper sulfate and 4kg sodium molybdate, and finally stir evenly until all the substances are completely dissolved, Sterilize at 130°C for 34 minutes to form the final culture medium.

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PUM

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Abstract

The invention discloses a formula and a preparation process of a medium for culturing medical shiraia bambusicola henn and belongs to the technical field of processing of mediums. The formula is characterized in that the medium contains 21 to 37 percent of nutrient solution, 8 to 19 percent of sugar, 2 to 11 percent of fungus, 5 to 16 percent of synergistic component and 30 to 56 percent of mineral water. According to the formula and the preparation process of the medium for culturing the medical shiraia bambusicola henn, the medium contains various nutritional components which are required by the survival of shiraia bambusicola henn, the nutrients required by growth of shiraia bambusicola henn can be provided, the culture period can be shortened, the yield and the quality of hypocrellin can be improved, the medium is good in culture effect, the quality of the cultured hypocrellin does not have obvious difference with that of the shiraia bambusicola henn hypocrellin in nature, and the medium is easy and convenient to prepare, safe and effective, convenient to use, and low in production cost. The formula of the medium can be applied to artificial culturing of shiraia bambusicola henn hypocrellin in a laboratory and industrial large-area production and development of shiraia bambusicola henn hypocrellin.

Description

technical field [0001] The invention belongs to the technical field of medium processing, and in particular relates to a medium formula and a preparation process for cultivating the medicinal fungus bamboo yellow. Background technique [0002] Bamboo yellow is a parasitic fungus of the Hypocreataceae family. Its sub-seat is warm in nature and light in taste. It is commonly known as bamboo flower, bamboo red dumpling, bamboo notoginseng, blood notoginseng, bamboo ginseng, bamboo cocoon or bitter bamboo flower. , Expelling wind and dampness, invigorating the middle and nourishing qi, promoting blood circulation, dispelling blood stasis, dredging menstrual flow, activating blood circulation, nourishing qi and blood, breaking blood stasis, relieving pain, restoring tissue function, enhancing physical fitness, and mainly treating deficiency cold stomach pain, rheumatoid arthritis pertussis, sciatic nerve Pain, bruises, sore muscles and bones, numbness of limbs, back strain, anemi...

Claims

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Application Information

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IPC IPC(8): C05G3/00
Inventor 林海萍张昕周湘
Owner ZHEJIANG FORESTRY UNIVERSITY
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