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Trichoderma viride strain and application thereof

A technology of Trichoderma viride and Botrytis cinerea, applied in the field of biological control preparations, can solve the problems of decreased control efficiency, environmental pollution, pesticide residues, etc., and achieve the effects of strong stress resistance, increased crop yield, and efficient control

Active Publication Date: 2014-08-13
BEIJING LEILI MARINE BIOINDUSTRY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical control and disease-resistant varieties are currently only effective against a small number of soil-borne diseases, and have little effect on most diseases, and the use of chemical pesticides will also cause pesticide residues and cause environmental pollution; at the same time, the long-term use of chemical pesticides makes pathogenic bacteria on them Drug resistance, leading to decline in control efficacy or even failure

Method used

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  • Trichoderma viride strain and application thereof
  • Trichoderma viride strain and application thereof
  • Trichoderma viride strain and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. Separation and purification of Trichoderma viride LLM-001 (CGMCC No.5044)

[0046] The Trichoderma viride LLM-001 (CGMCC No.5044) of the present invention is obtained from Wulian County mushrooms using a plate streaking method, and the separation method is:

[0047] ⑴ Isolation of Trichoderma strains: Take the seriously damaged mushroom bag, first treat it locally with 75% alcohol for 1 min, and then treat it with a UV lamp on an ultra-clean workbench for 20 minutes, then cut the treated part with a sterile blade and inoculate it with Pick a small amount of Trichoderma spores with a needle, and streak on the PDA plate to observe the growth of the colony regularly. Then use the plate streak method to purify the Trichoderma strain and transfer it to the slope of the PDA test tube for storage.

[0048] ⑵ Screening of Trichoderma strains against cucumber gray mold

[0049] ①Preliminary screening: Prepare a PDA plate by using the confrontation culture method. Use a punch to make...

Embodiment 2

[0067] 1. Fermentation process of Trichoderma viride

[0068] PDB medium formula: 200g potato (peeled), 20g glucose, 1000mL distilled water.

[0069] A large number of solid fermentation medium formula (quality percentage):

[0070] Solid material: corn cob 60%, bran 40%

[0071] Inorganic salt solution: 3.5% potassium dihydrogen phosphate, 0.04% magnesium sulfate, 4% ammonium sulfate, and the rest is water.

[0072] The solid-liquid ratio is 1:1.8 (mass ratio)

[0073] Trichoderma viride LLM-001 (CGMCC No.5044) mass solid fermentation process:

[0074] ①Bacterial seed solution culture: Pick a small amount of spores from the slope of the test tube from Trichoderma viride (LLM-001) CGMCC No.5044, move them to the PDB liquid medium, and culture with shaking at 28°C for 3~5 days , This is the seed liquid.

[0075] ② Cultivation of solid-producing strains: Inoculate the seed liquid into a solid medium (500 mL Erlenmeyer flask) at a ratio of 10%, and cultivate at a constant temperature of 28°C...

Embodiment 3

[0078] This example provides related experiments on the use of Trichoderma viride LLM-001 (CGMCC No. 5044) (hereinafter referred to as Trichoderma viride LLM-001) raw powder to prevent disease and increase yield of cucumber gray mold.

[0079] 1. Test medicine

[0080] The original powder of Trichoderma viride LLM-001 (prepared in Example 2) 40% Imidacloprid wettable powder (produced by Shandong Shouguang Shuangxing Pesticide Co., Ltd., commercially available).

[0081] 2. Test crops and control objects

[0082] The test crop is cucumber, the variety is Changchun dense thorn;

[0083] The control object is gray mold.

[0084] 3. Conditions of the test site

[0085] The test site was set up in the cucumber greenhouse planting base in Shouguang, Shandong Province. The cucumber gray mold disease in the greenhouse has been severe in recent years and its prevention and control is difficult. The experiment was conducted in a 600 square meter (0.9 mu) cucumber greenhouse. The soil quality is lig...

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Abstract

The invention provides a Trichoderma viride strain and application thereof. TheTrichoderma viride provided by the invention has a preservation number of CGMCC No.5044, and characteristics of fast growth, large sporulation capacity, wide action spectrum, strong stress resistance, and rapid colonization in large quantity in rhizosphere of plants, and can be used for the prevention and control of plant soil-borne diseases. A biological control preparation prepared from the Trichoderma viride can not only efficiently control the soil-borne plant diseases but also effectively increase the yield of crops, and has promising application prospects.

Description

Technical field [0001] The invention belongs to the field of biological control of plant diseases. Specifically, the present invention relates to a Trichoderma viride capable of efficiently preventing and controlling plant soil-borne diseases and its use, and a biological control agent prepared by using the Trichoderma viride. Background technique [0002] Plant soil-borne diseases are an important type of plant diseases, including root rot, blight, damping-off, wilt and so on. Fungi, bacteria, nematodes, and viruses are all soil-borne pathogens that cause soil-borne diseases. They usually infect plant roots, cause plant roots and even the entire plant disease, and cause significant economic losses. [0003] For the prevention and control of soil-borne plant diseases, conventional methods include traditional chemical control, breeding of disease-resistant varieties, and biological control. Chemical control and disease-resistant varieties are currently only effective against a few...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
Inventor 汤洁
Owner BEIJING LEILI MARINE BIOINDUSTRY INC
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