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Application of kalimeris in preparation of medicament for reducing blood fat or functional foods

A technology for functional food and hypolipidemic drug, applied in the field of functional food and phytochemistry, can solve the problems of large sample loss, cumbersome process, low yield, etc., to achieve the effect of preventing free radical damage and reducing accumulation

Inactive Publication Date: 2012-12-19
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process of these methods is too cumbersome, and the sample loss is also large (resulting in low yield); in addition, a large amount of solvent elution will also greatly increase the cost; and the separation and purification of silica gel column chromatography and Sephadex LH-20 chromatographic column are difficult for industrial production

Method used

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  • Application of kalimeris in preparation of medicament for reducing blood fat or functional foods
  • Application of kalimeris in preparation of medicament for reducing blood fat or functional foods
  • Application of kalimeris in preparation of medicament for reducing blood fat or functional foods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1: The whole plant of Malan is dried, crushed, sieved through 20 mesh, weighed 500g into the reactor, added 7000ml of 80% ethanol, heated in a water bath to 60°C, stirred and extracted (reflux extraction, or immersion extraction, or percolation extraction ) for 60 min, filter, collect the extract, extract the residue once more under the same conditions, combine the two extracts, and concentrate under reduced pressure to remove ethanol. Sequentially extract twice with 3 times the volume of petroleum ether and ethyl acetate, combine the ethyl acetate extracts, and concentrate to obtain a paste; the paste is separated by a macroporous adsorption resin column, first rinsed with water and 20% ethanol in sequence, Impurities were removed, and then eluted with 40%-60% ethanol solution, the ethanol eluate was collected, concentrated under reduced pressure, freeze-dried, and Malan extract T was obtained.

[0032] Determination of extract content: taking oleanolic acid as...

Embodiment 2

[0034] Example 2: The whole plant of Malan is dried, crushed, sieved through 20 mesh, weighed 500g into the reactor, added 7000ml of 80% ethanol, heated in a water bath to 60°C, stirred and extracted (reflux extraction, or immersion extraction, or percolation extraction ) for 60 min, filter, collect the extract, extract the residue once more under the same conditions, combine the two extracts, and concentrate under reduced pressure to remove ethanol. Sequentially extract twice with 3 times the volume of petroleum ether and ethyl acetate, combine the ethyl acetate extracts, and concentrate to obtain a paste; the paste is separated by a macroporous adsorption resin column, first rinsed with water and 20% ethanol in sequence, The ethanol eluate was collected, concentrated under reduced pressure, freeze-dried, and Malan extract F was obtained.

[0035] Determination of extract content: taking oleanolic acid as a standard product, using the vanillin-glacial acetic acid-perchloric a...

Embodiment 3

[0036] Embodiment 3: Embodiment 3: hypolipidemic efficacy test:

[0037] 3.1 Test animals and feed formula

[0038] 3.1.1 Test animals

[0039]Healthy and clean male mice (CD-1), four weeks old, weighing 18-22g, 72 in total, provided by the Experimental Animal Center of Nanjing Medical University, certificate number: SYXK (Su) 2008-2007, the mice were bred in The aseptic air-conditioned room in the animal center of Nanjing Medical University, room temperature 19-21 ℃, relative humidity 50-60%, automatic control day and night 12h, free drinking water.

[0040] 3.1.2 Feed formulation

[0041] The basic feed complies with the national animal feeding standards and is provided by the Experimental Animal Center of Nanjing Medical University. Its main nutritional components are shown in Table 1.

[0042]

[0043] 3.2 Malan component animal model in vivo test

[0044] Malan crude extract was successively extracted with petroleum ether and ethyl acetate, and the ethyl acetate pa...

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Abstract

The invention relates to a preparation method for triterpenoid compounds in kalimeris and a function for reducing blood fat of the triterpenoid compounds. The invention provides a method for extracting and preparing the triterpenoid compounds from the kalimeris as well as a blood-fat reducing function and application of the triterpenoid compounds obtained by the method. The method for preparing the triterpenoid compounds in the kalimeris comprises the following steps of: with a full kalimeris plant as a raw material, carrying out reflux extraction or maceration extraction or percolation extraction with 80 percent ethanol; filtering and carrying out vacuum concentration on filtrate; sequentially extracting with petroleum ether and ethyl acetate; enabling a concentrated solution of the ethyl acetate extraction part to pass through a macroporous absorption resin chromatograph column; sequentially carrying out water washing and eluting with 20 percent ethanol for removing impurities; then eluting with 40-60 percent ethanol solution; collecting an ethanol elution solution; and carrying out vacuum concentration, freezing and drying on the ethanol elution solution to obtain an extract of the triterpenoid compounds in the kalimeris. The total content of the triterpenoid compounds as the extract of the kalimeris is not lower than 50 percent; and the triterpenoid compounds comprise main components of suberone, epifriedelanol, sitosterol, stigmasterol, spinach sterol, spinach sterone and daucosterol and have remarkable efficacy of reducing the blood fat.

Description

Technical field [0001] The patent of the invention involves the separation preparation of natural active factors and the application of functional foods in the field of functional food and plant chemistry. Background technique [0002] Cardiovascular and cerebrovascular diseases have become the largest killer that harms human life and health today, and the world's death is the first in the world.Excessive cholesterol concentration in plasma is a major cause of cardiovascular and cerebrovascular diseases.At present, blood lipid -lowering drugs have to varying degrees of toxic and side effects, such as gastrointestinal reactions, liver, kidney injury, etc. Most lipid -lowering western medicines can concentrate blood lipids on liver metabolism, making lipids more accumulated in the liver and aggravated liver cells to aggravate liver cellsFat degeneration and liver function damage make patients reduce the liver and protect the liver during treatment.In addition, most chemical synthet...

Claims

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Application Information

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IPC IPC(8): A61K36/28A61P3/06A23L1/29A23L33/00
Inventor 吕丽爽刘跃钧刘新明
Owner NANJING NORMAL UNIVERSITY
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