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Novel process for fermenting coenzyme Q10 based on online oxygen consumption rate control

A technology of oxygen consumption rate and new process, applied in the field of fermentation, can solve the problem of not being able to become the control index of oxygen supply level, etc., and achieve the effects of high specific production rate, high substrate conversion rate, and improved yield

Active Publication Date: 2013-01-16
SHANGYU NHU BIOCHEM IND +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the general aerobic fermentation process, a higher oxygen supply is controlled to avoid the occurrence of oxygen limitation. In this case, the dissolved oxygen concentration (Dissolved Oxygen, referred to as DO) can be used to characterize the oxygen supply level. [7] , DO can be effectively controlled by controlling the stirring speed and air flow rate, but for microorganisms with high oxygen consumption characteristics, the dissolved oxygen concentration is often below the critical oxygen concentration level during the fermentation process and remains unchanged, which cannot be an effective oxygen supply. Level control index, which requires looking for another parameter that can effectively characterize the oxygen supply level

Method used

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  • Novel process for fermenting coenzyme Q10 based on online oxygen consumption rate control
  • Novel process for fermenting coenzyme Q10 based on online oxygen consumption rate control
  • Novel process for fermenting coenzyme Q10 based on online oxygen consumption rate control

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Embodiment 1

[0053] 1 Materials and methods

[0054] 1.1 Bacteria and culture medium

[0055] Species: Rhodobacter sphaeroides, the deposit number is CGMCC No.5997, CGMCC No.5998, CGMCC No.5999.

[0056] Seed medium (g / L): 1g yeast powder, 1gNH 4 Cl, 1g sodium chloride, 2.8mg ferric citrate, 0.6g KH 2 PO 4 ,0.9g K 2 HPO 4 ,0.25g MgSO 4 ,0.1g CaCl 2 , 0.5ug biotin, pH 7.0

[0057] Fermentation medium (g / L): 8g yeast powder, 1g NH4Cl, 1g sodium chloride, 5mg ferric citrate, 0.6g KH 2 PO 4 ,0.9gK 2 HPO 4 ,0.5g MgSO 4 ,0.1g CaCl 2 , 3ug biotin, pH 7.0.

[0058] Feed medium 1 (g / L): 200gKH 2 PO 4 .

[0059] Feed medium 2 (g / L): 700 g glucose.

[0060] 1.2 Reagents and instruments

[0061] Reagents: corn steep liquor (Shanghai Xiwang Starch Sugar Co., Ltd.), glucose (Shanghai Sugar Co., Ltd.), and other reagents are of domestic analytical grade.

[0062] Instrument: Model 722 UV-Vis Spectrophotometer; HPLC 1100 (Agilent); Tail Gas Mass Spectrometer: American Extrel Process Ma...

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Abstract

The invention provides a novel process for fermenting coenzyme Q10 based on online oxygen consumption rate control. Oxygen consumption rate is controlled at 30-150mmol / L*h during fermentation of coenzyme Q10 strain production, so that growth of thallus and starting and accumulation of coenzyme Q10 synthesis are promoted. Yield of coenzyme Q10 is evidently increased, unit energy consumption is lowered, and production cost is lowered greatly. The novel process is simple to control, high in operability, energy-saving and the like.

Description

technical field [0001] The invention relates to the field of fermentation, and more particularly to an optimized new process for coenzyme Q10 fermentation based on the online oxygen utilization rate. Background technique [0002] coenzyme Q 10 (CoQ 10 ) has another name called ubiquinone, decene quinone, the chemical name is 2,3-dimethoxy-5-methyl-6-decyl isopentenyl quinone, and its structural formula is as follows: [0003] [0004] coenzyme Q 10 Its biological activity comes from the redox properties of its quinone ring and the physicochemical properties of its side chains. It is a natural antioxidant and cell metabolism activator produced by cells itself. It has antioxidant properties, eliminates free radicals, improves immunity, Anti-aging and other functions. It is widely used in the treatment of various heart diseases, cancer, diabetes, acute and chronic hepatitis, Parkinson's disease and other diseases, and also has many applications in cosmetics and anti-agin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/66C12R1/01
Inventor 陈召峰胡伟江李永陈晓炳吕勇吕家樑李伟锋杨梢烽王泽建郭美锦
Owner SHANGYU NHU BIOCHEM IND
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