New method for using model organism zebrafish to research metabolism of glucoside compound asperosaponin VI

A technology for saponins and compounds of Chuanxujuan, which is applied in the field of compound metabolism research to achieve the effects of strong operability, high accuracy and guaranteed accuracy.

Inactive Publication Date: 2013-01-16
JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE +1
View PDF7 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recent studies have shown that zebrafish still have phase II metabolic enzymes [Y.Morcillo, G.Janer.S.C.M.O'Hara, D.R.Livingstone, C.Porte.Environmental Toxicology and Chemistry, 23(4), 990-996(2004); D.V.Almeida, B.F.Nornberg, L.A.Geracitano, D.M.Barros, J.M.Monserrat, L.F.Marins.Fish Physiol Biochem.36(3):347-53(2010)], we published in 201210187191.8 the use of zebrafish to study the flavonoids chrysin, 5 - Phase II metabolism of hydroxyflavones and 7-hydroxyflavones, found their glucuronic acid conjugates and sulfuric acid conjugates phase II metabolites; in addition, zebrafish also have intestinal flora [Lv Aijun, Yang Zhengxing, Liu Huan, Hu Xiucai, Zhang Yanhua, Cheng Chao. China Agricultural Science Bulletin, 2010, 26(24): 412-415], whether this kind of flora can cause glycoside compounds to undergo a deglycosylation reaction similar to that of mammalian intestinal flora, domestic research on this research is still blank

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • New method for using model organism zebrafish to research metabolism of glucoside compound asperosaponin VI
  • New method for using model organism zebrafish to research metabolism of glucoside compound asperosaponin VI
  • New method for using model organism zebrafish to research metabolism of glucoside compound asperosaponin VI

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Study on the Metabolism of Dipsacus saponin VI Using the Model Organism Zebrafish

[0042] 1. Materials and Instruments

[0043] Test drug: Dipsacus saponin VI (Asperosaponin VI)

[0044] Preparation method: Weigh 3-4 mg equivalent of Dipsacus saponin VI and dissolve in 1 ml of dimethyl sulfoxide (DMSO), add 250 ml of Robust purified water, and shake well.

[0045] Animal: Zebrafish, provided by the Institute of Model Biology, Nanjing University.

[0046] Reagents: acetonitrile and formic acid are chromatographically pure (Germany, Merck Company), dimethyl sulfoxide (DMSO, Sinopharm Chemical Reagent Co., Ltd.), ultrapure water Robust purified water.

[0047] Instruments: Agilent 1100 series high performance liquid chromatography, including G1311A quaternary gradient pump, G1313A autosampler, G1316A column thermostat, G1315B DAD detector; Chemstation 6.01 chromatographic workstation (Agilent, USA). Waters Alliance 2695-ZQ 2000 liquid chromatography-mass spec...

Embodiment 2

[0061] Embodiment two studies the metabolism of Dipsacus saponin VI with rats

[0062] Existing in vivo mammalian metabolism studies of Dipsacus saponin VI are not comprehensive, except for the literature [LI Kai, YANG Xiao-Lin, ZHANG Chun-Feng and YANG Zhong-Lin. Chin J Nat Med, 2009, 7(6): 440-443.] In addition to the metabolites in feces after oral administration of Dipsacus saponin VI in rats, the analysis of metabolites in plasma, bile, and urine has not been seen. Metabolism differences of saponin VI, we conducted a systematic study on the metabolism of Dipsacus saponin VI in rats.

[0063] 1. Materials and Instruments

[0064] Test drug: Dipsacus saponin VI compound

[0065] Preparation of the test drug: Weigh an appropriate amount of Dipsacus saponin VI, add 0.8% CMC-Na to make 10mg·mL -1 solution for gavage in rats.

[0066] Heparin was purchased from Jiangsu Wanbang Biochemical Pharmaceutical Co., Ltd., normal saline (sodium chloride injection, Nanjing Xiaoying P...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a new method for using a model organism zebrafish to research deglycosylated metabolism of glucoside compound asperosaponin VI. The new method comprises the following steps of selecting a zebrafish as a subject of a drug metabolism research, carrying out an experimental grouping design by an optimized experimental grouping method, dosing through an optimized medicine dose way, and extracting asperosaponin VI metabolin metabolized by the zebrafish through an optimized method, especially analyzing the metabolin by the optimized analysis method, so that the entire experimental method is strong in operability and is capable of objectively responding a true deglycosylated metabolism condition of a medicine in vivo, particularly the glucoside compound asperosaponin VI. In addition, the hydroxylated metabolism of the glucoside compound asperosaponin VI is high in accuracy of the experimental results, is capable of overcoming the defect that general in-vitro metabolic experiments are difficult to embody in aggregate results in vitro and the defects that the general in-vivo metabolic experiments are large in dosage and high in labor intensity, and the experimental method is strong in repeatability, required tested drug dose is little, cost is low, the labor intensity is low, a range of application is wide, and the method can be experimentized and researched in batches and working efficiency is high.

Description

technical field [0001] The invention relates to a method for studying the metabolism of a class of compounds, in particular to a new method for studying the metabolism of the glycoside compound Dipsapacea saponin VI by using the model organism zebrafish. Background technique [0002] Glycoside compounds often refer to glycoside compounds combined with aglycone and sugar, such as saponins, flavone glycosides, etc., which are important natural products, widely distributed in plants, and have important biological activities, such as protecting the cardiovascular system, antibacterial and antibacterial. Virus, anti-tumor activity, anti-inflammation, anti-oxidation, liver protection, kidney and bone strengthening and other activities, glycoside compounds in the body are usually deglycosylated by the gastrointestinal flora in the body, and converted into hypoglycosides or even aglycones, improving activity while increasing bioavailability. [0003] Drug metabolism is usually divi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/15
Inventor 韦英杰李萍贾晓斌齐炼文王长梅彭蕴茹舒娈
Owner JIANGSU PROVINCE INST OF TRADITIONAL CHINESE MEDICINE
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap