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Streptococcus thermophilus capable of lowering cholesterol levels and application thereof

A Streptococcus thermophilus, cholesterol-lowering technology, applied in the field of Streptococcus thermophilus, can solve problems such as intolerance to bile salts of gastric acid, achieve no adverse reactions, good effect, and remarkable effect

Active Publication Date: 2013-01-30
山东宝来利来生物工程股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most lactic acid bacteria cannot tolerate gastric acid and bile salts in the intestines. As the lactic acid bacteria that can lower cholesterol, they must pass through the human gastrointestinal tract to exert the effect of lowering cholesterol.

Method used

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  • Streptococcus thermophilus capable of lowering cholesterol levels and application thereof
  • Streptococcus thermophilus capable of lowering cholesterol levels and application thereof
  • Streptococcus thermophilus capable of lowering cholesterol levels and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment one, the isolation identification of strain

[0041] With the improvement of people's living standards, the intake of high-cholesterol diets has gradually increased, and the resulting cardiovascular diseases such as hyperlipidemia, coronary heart disease, and atherosclerosis have seriously endangered human health. There are exogenous intake and endogenous synthesis of cholesterol in the human body. Research on how to reduce the intake of exogenous cholesterol and reduce the level of serum cholesterol in the human body has attracted more and more attention. In addition to restricting diet, domestic and foreign scientists have shown that lactic acid bacteria can lower human serum cholesterol. The use of probiotics to lower cholesterol is non-toxic, has no side effects, and can be eaten safely for a long time. It is a new way to solve human cardiovascular diseases.

[0042] 1 Materials and methods

[0043] 1.1 Test material

[0044] Yogurt and kimchi: purchase...

Embodiment 2

[0077] Embodiment two, the physicochemical property analysis of strain

[0078] 1 Materials and methods

[0079] 1.1 Strains: Lactobacillus acidophilus 1.2467, Lactobacillus plantarum LP, Streptococcus thermophilus BLST (isolated in Example 1), Streptococcus thermophilus 1.1855, Lactobacillus reuteri 6126, Lactobacillus rhamnosus 6133, Lactobacillus bulgaricus BJLY (isolated from the research institute of Shandong Baolai Lilai Bioengineering Co., Ltd.), Lactobacillus acidophilus R3 (isolated from the research institute of Shandong Baolai Lilai Bioengineering Co., Ltd.).

[0080] 1.2 Reagents: Cholesterol (analytical pure), glacial acetic acid, sulfuric acid, o-phthalaldehyde, n-hexane, potassium hydroxide, etc. are analytically pure; bile salts (biochemical reagents), Tween-80, glucose, beef extract, peptone, acetic acid Sodium, Ammonium Citrate, Dipotassium Phosphate, Magnesium Sulfate.

[0081] 1.3 Culture medium: MRS medium

[0082] Preparation of in vitro cholesterol-lo...

Embodiment 3

[0206] Embodiment three, the research of fermentation process

[0207] Study on Fermentation Technology of Streptococcus thermophilus BLST

[0208] Bacteria powder is obtained through the following steps:

[0209] (ⅰ) Strains: Streptococcus thermophilus BLST (Streptococcus thermophilus)

[0210] (ii) Slant culture: inoculate the lyophilized powder strains on a solid slant medium, and culture at 37°C for 28 hours;

[0211] (Ⅲ) Primary seed cultivation: Take the cultivated slant, inoculate two rings with an inoculation loop into 50 mL of seed liquid medium under sterile conditions, and culture at 37°C for 22 hours to obtain primary seeds liquid;

[0212] (iv) Expansion cultivation: Inoculate the primary seed solution with 3% inoculation amount into 500mL seed liquid medium, and culture it at 37°C for 22 hours to obtain the secondary seed solution;

[0213] (ⅴ) Fermentation tank culture: Inoculate the secondary seed liquid into the liquid fermentation medium with a 3% inocula...

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Abstract

The invention discloses a strain of streptococcus thermophilus capable of lowering cholesterol levels. The streptococcus thermophilus has a classification name of streptococcus thermophilus BLST, and was collected in China Center for Type Culture Collection with the collection number of CCTCC NO.M 2012282 on July 11th, 2012. Tests prove that the streptococcus thermophilus has a cholesterol lowering function, so that the bacterial strain of the streptococcus thermophilus can be used as medicine or dietary supplements or be used in preparation of medicine or dietary supplements. During specific application, the streptococcus thermophilus BLST can be cultured, and thus fermentation products, namely the dietary supplements for lowering the cholesterol levels, are obtained. The fermentation products can also serve as raw materials of the medicine or the dietary supplements for lowering the cholesterol levels. The invention also provides a streptococcus thermophilus preparation named Baiao Zhile capable of lowering cholesterol levels, and the preparation consists of the following raw materials: hawthorn fruit, ganoderma lucidum spores powder, red kojic rice powder, fructooligosaccharide, xylooligosaccharide, streptococcus thermophilus BLST powder and lactobacillus plantarum LP-11 powder.

Description

technical field [0001] The invention relates to a streptococcus thermophilus with cholesterol-lowering ability and application thereof. Background technique [0002] Cholesterol is an indispensable and important substance in animal tissue cells, especially in the brain and nerve tissues. It not only participates in the formation of cell membranes, but also is a raw material for the synthesis of bile acids, vitamin D and steroid hormones. Cholesterol has a wide range of physiological functions in the body . However, with the improvement of people's living standards, the intake of high-cholesterol diets is gradually increasing, but the subsequent negative impact is the incidence of cardiovascular and cerebrovascular diseases such as atherosclerosis, coronary heart disease, and hypertension. keep improving. Hyperlipidemia is the main cause of atherosclerosis and coronary heart disease, and the incidence of cardiovascular and cerebrovascular diseases such as atherosclerosis, c...

Claims

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Application Information

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IPC IPC(8): C12N1/20A61K35/74A61K36/734A61P3/06A23L1/29C12R1/46C12R1/25A23L33/00A61K35/744
Inventor 单宝龙谷巍程秀芳翟延庆王丛丛刘虹李丹谢莉敏
Owner 山东宝来利来生物工程股份有限公司
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