Method for synchronously separating liquiritin and liquiritin apioside from liquorice

Abstract

The invention relates to a method for preparing active ingredients from Chinese medicinal herbs, in particular to a method for synchronously separating liquiritin and liquiritin apioside from liquorice. The method comprises the steps of taking the liquorice as a raw material, conducting heating reflux or ultrasonic extraction, macroporous resin series adsorption and gradient desorption, then conducting secondary adsorption and desorption after recovering organic solvent from an analytic solution, and finally obtaining the liquiritin and the liquiritin apioside simultaneously with purities of 90%-98% through a medium-pressure gradient elution system. The method is short in process flow, simple to operate and low in cost, can simultaneously separate the high-purity liquiritin and liquiritin apioside, and is applicable to larger-scale preparation.

Description

technical field [0001] The invention relates to a method for preparing active ingredients from Chinese herbal medicines, in particular to a method for synchronously separating liquiritin and liquiritin celery from licorice. Background technique [0002] Licorice is a leguminous plant Glycyrrhiza uralensis Fisch. , Glycyrrhiza inflata Bat. Or Glycyrrhiza glabra L. dry roots and rhizomes. Clinical applications include licorice and roasted licorice. Among them, licorice has the functions of invigorating the spleen and replenishing qi, clearing away heat and detoxification, reducing phlegm and relieving cough, relieving spasm and relieving pain, etc. Licorice mainly contains saponins and flavonoids. Studies have found that licorice flavonoids have antibacterial, anti-oxidant, anti-tumor, anti-viral and other physiological activities. Liquiritin is the main flavonoid component in licorice, which has antiviral, antioxidative and antiarrhythmic effects. At the same time, liqui...

AI Technical Summary

Problems solved by technology

[0003] In the prior art, there are many methods for separating and purifying active ingredients, mainly chromatography, but there are great differences in the types and structures of active ingredients contained in different plants, so there are differences in chemical properties. General methods are used in the extraction and purification process. In the extraction and purification method, the purity of the active ingredient obtained is not high. If a high purity is to be achieved, the separation efficiency is low and the cost is high

At present, there are few reports on the extraction and purification of high-purity liquiritin and liquiritin-galloside from licorice, and the chemical components in licorice are complex, which is very difficult in the extraction and separation process

The publication number is CN101289480, and the invention name is a patent for the separation and preparation method of liquiritin, in which the patent uses water extraction, alcohol precipitation, membrane separation, and then uses macroporous resin to separate liquiritin; the publication number is CN102050851A, and the invention name is liquiritin and its preparation method The patent is to extract liquiritin from glycyrrhizic acid powder, extract it with dilute ammonia water, extract it with organic solvent, and then separate it with polyamide resin; the publication number is CN102391330A, and the invention name is the patent of the method of extracting liquiritin from licorice. , over-mixed resin column separation to obtain liquiritin, but these methods all adopt the single-stage separation mode, and the yield is low, so it is not suitable for large-scale production; the application number is 200710011041.0 Invention name A kind of preparation method of liquiritin discloses that the licorice medicinal material is passed through water Extraction, alcohol precipitation, separation by molecular weight membrane separator, macroporous resin column, and high-efficiency industrial chromatographic column, and freeze-drying to obtain liquiritin. Although this method has a large amount of preparation at one time, the method still adopts a single-stage separation method, and the production efficiency is high. low, and only obtained liquiritin, resulting in a waste of resources

[0004] In short, the existing methods all have the disadvantages of intermittent and discontinuous operation, low utilization rate of upper column liquid and eluent, etc. Reasonable use of the advantages of selective adsorption separation of resin and optimization of separation process will greatly reduce production costs and improve production Efficiency, non-toxic and environmental protection, open up a new industrial pattern for the preparation process of liquiritin and liquiritin celulose

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