Method for preparing liquid mycelium fermentation culture medium formula of selenium-rich cordyceps militaris

A technology for mycelium fermentation and culture medium formulation, which is applied in fertilizer mixtures, fertilization devices, applications, etc., can solve the problems of mycelium toxicity, difficulty in mycelium absorption, etc., and achieves less bacterial contamination and nitrogen source cost reduction. , Reasonable effect of nitrogen source components

Inactive Publication Date: 2013-04-10
浙江山清水秀农业开发有限公司
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AI Technical Summary

Problems solved by technology

In the traditional cultivation process of selenium-enriched Cordyceps militaris mycelium, inorganic selenium sodium selenite is generally added to the culture medium, which is converted into organic selenium after being absorbed by the mycelium. This method is not only difficult for the mycelium to absorb , Sodium selenite is also toxic to mycelia and has great defects

Method used

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Embodiment Construction

[0021] Crush the cocoon clothes and cocoon shells of the dried natural colored cocoons into pieces of 1-3mm in size, then measure distilled water 30 times the mass of the raw materials, add Na 2 CO 3 Formulated with a concentration of 0.3% Na 2 CO 3 solution, immerse cocoon clothing and cocoon shell raw material fragments in the solution, boil at normal pressure and maintain for 40 minutes, stop heating, and cool the solution to normal temperature. After adjusting the pH value of the sericin solution to neutral with dilute hydrochloric acid, gradually add 15% Ca (OH) 2 solution until a sericin precipitate appears. Precipitate and separate the sericin protein and place it in a - 20 ℃ refrigerator, and vacuum-dry it directly after freezing to obtain the dried sericin protein, crush it to 200 mesh, and obtain the colored cocoon sericin rich in flavonoids and carotene protein.

[0022] Commercially available selenium-enriched yeast with a selenium content of 2000 mg / ...

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Abstract

The invention relates to a method for preparing a liquid mycelium fermentation culture medium formula of selenium-rich cordyceps militaris. The method takes natural colored silkworm cocoon sericin protein and a selenium-rich yeast as a main nitrogen source, takes mixed coarse food grains including rice, Chinese yam, potatoes, wheat, millet and corn as a main carbon source, takes organic selenium in the selenium-rich yeast as a selenium source, takes lentinan and adenosine as motivating factors, and takes other trace factors as auxiliary factors to manufacture the liquid mycelium fermentation culture medium formula of selenium-rich cordyceps militaris. The method effectively recycles abandoned husks and cocoon shells, the cost of the nitrogen source is greatly reduced, the nitrogen source in a culture medium has good bacterial resistance and antioxidant activity, and the fermentation process is not easily contaminated by bacteria. The selenium-rich yeast in the culture medium plays a role of supplementing the nitrogen source and is also a selenium source. Through the addition of the lentinan and the adenosine, the metabolism and growth of selenium-rich cordyceps militaris mycelium can be significantly improved, and the output of active materials such as cordyceps polysaccharide, cordycepic acid, cordycepin and the like is greatly increased.

Description

technical field [0001] The invention relates to a preparation method of a novel medium formula for liquid fermentation of Cordyceps militaris, in particular to a preparation method of a liquid mycelium fermentation medium formula of selenium-enriched Cordyceps militaris, belonging to the technical field of biopharmaceutical production. Background technique [0002] Cordyceps militaris ( C o r d c e p s mi l i t a r i s ) of the genus Ascomycota ( A s c o my c o t i n a ) Ergotaceae ( C l a v i e i p i t a e e u e ) Cordyceps ( C o r d y c e p s ), is a medicinal fungus belonging to the same genus as Cordyceps sinensis, and the number of natural resources distributed in the world is very small. Cordycepin (3'-deoxyadenosine), cordycepic acid, and polysaccharides in Cordyceps militaris have significant effects such as antibacterial, anti-inflammatory, anti-aging, anti-tumor, immune regulation, and regulation of blood lipid and blood sugar, and are important active ingre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G1/00
Inventor 李卫旗丁农袁康
Owner 浙江山清水秀农业开发有限公司
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