Fungal glucan oligomer chrome complex and preparation method thereof

A technology of chromium complex and oligoglucose, which is applied in the field of chromium oligosaccharide and its preparation, can solve the problems of large difference in ratio, harsh reaction conditions, and low bioavailability, and achieve mild synthesis conditions, The conditions are easy to control and the effect of low molecular weight

Inactive Publication Date: 2013-04-24
BINZHOU UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0020] To sum up, there are many kinds of organic chromium with biological activity. However, because their solubility and intestinal absorption ratio vary greatly, their biological activities vary. Some have poor solubility and low bioavailability, and some anionic complexes There are adverse effects on the human body, and the reaction

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0052] Example 1

[0053] Preparation of Sclerotium β-oligosaccharide

[0054] Suspend 15 g of Sclerotium dextran in 100 mL of water, stir to fully swell, adjust the pH to 4-5, heat to 50°C, add 30 mg of β-glucanase, and hydrolyze for 1-2 hours under stirring; 10g of glycans, keep stirring for 1-2h, the solution becomes thin and gradually transparent; adjust the pH to 7-8, add 20mg of neutral protease, keep for 2-4h; heat up to 90℃, inactivate the enzyme; centrifuge at 6000r / min for 20min, Use an ultrafiltration membrane with a molecular weight cut-off of 10kDa to collect the filtrate, and then use a nanofiltration membrane with a molecular weight cut-off of 200 Dalton to collect the retentate; that is, the weight-average molecular weight of 2528 Dalton oligosaccharides. The oligoglucose solution contains 22.51% (w / v) of β-oligoglucose.

Example Embodiment

[0055] Example 2

[0056] Synthesis and Purification of Sclerotium β-oligosaccharide Chromium

[0057] Sclerotium β-oligosaccharide solution 100mL, containing 15.37% (w / v) of oligoglucose with a weight average molecular weight of 2240Dalton, adjust the pH to 12 with 10mol / L NaOH, heat to 75℃, and add 2mol / L dropwise During the period, 2mol / L NaOH was used to adjust the pH value to stabilize the pH at about 12. When the green flocculent precipitation just appeared in the solution, the dripping of chromium chloride and NaOH was stopped, and the reaction solution continued to be at 75℃ Keep for 1h. Ultrafiltration with a nanofiltration membrane with a molecular weight cut-off of 200 Dalton was used to collect the retentate; after further purification by 200-300 mesh silica gel column chromatography, lyophilized to obtain 8.6 g of Sclerotium β-oligosaccharide chromium. The yield of β-oligosaccharide chromium is 56%, and the chromium content is 7.43% w / w.

[0058] The oligosaccharide c...

Example Embodiment

[0065] Example 3

[0066] Preparation of Aureobasidium pullulans α-oligosaccharide

[0067] Pullulan 10g, suspended in 100mL water, stir to fully swell, adjust the pH value 4-5, heat at 55℃, add 10mg α-1,6-glucanase, hydrolyze with stirring for 2h; then add 5g dextran , 10mg of α-amylase is continuously stirred for 1-2h, the solution becomes thin and gradually transparent; adjust the pH to 7-8, add 15mg of alkaline protease, keep for 2-4h; heat up to 100℃, inactivate the enzyme; centrifuge at 6000r / min 20min, ultrafiltration with an ultrafiltration membrane with a molecular weight cut-off of 6000 Dalton to collect the filtrate, and then ultrafiltration with a nanofiltration membrane with a molecular weight cut-off of 300 Dalton to collect the cutoff; that is, the weight-average molecular weight of 1650 Dalton oligosaccharides. The oligoglucose solution contains 12.6% (w / v) of α-oligoglucose.

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Abstract

The invention relates to the technical fields of bioengineering and food medicine, and particularly provides a glucan oligomer chrome complex prepared by using fungal glucan oligomer as a ligand compound and trivalent chrome as coordination center ions and a preparation method thereof. The prepared glucan oligomer chrome complex has the advantages of proper chrome content, high stability and favorable water solubility, can be easily absorbed, and is free of free Cr<6+> ions; and the deionized ligand compound has the advantages of no toxic or side effect and high bioactivity, and is an active substance with GTF like function and immunoregulation action.

Description

technical field [0001] The invention relates to the technical field of bioengineering, and specifically provides a glucooligosaccharide chromium complex prepared by using fungal glucooligosaccharide as a ligand compound and using trivalent chromium as a coordination center ion and a preparation method thereof. Background technique [0002] Chromium is an important trace element that has been widely studied and the conclusions are relatively consistent. The content of this trace element is significantly reduced in diabetic patients. The reason is that trivalent chromium can form glucose tolerance factor (glucose tolerance factor) in the human body. , GTF), cooperate with insulin to participate in the metabolism of sugar and lipid in human body. 1950s, Cr 3+ It has been proven to be the main active ingredient of GTF, and was identified as an essential trace element for the human body in the 1980s. The physiological function of chromium is mainly based on Cr 3+ The form of G...

Claims

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Application Information

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IPC IPC(8): C08B37/00C07H3/04C07H3/06C07H1/00C12P19/22C12P19/14A61P3/10
Inventor 王宝琴徐泽平
Owner BINZHOU UNIV
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