Aquatic product Edwardsiella tarda rapid drug allergy detection kit

Abstract

The invention relates to an Edwardsiella tarda rapid drug allergy detection kit which can be applied in aquaculture sites. The Edwardsiella tarda rapid drug allergy detection kit comprises a detection plate, a bacterium body collecting tool, bacterium increasing liquid, a drug allergy detection culture medium, a turbidimetric tube and a colorimetric card. The detection plate comprises a detection hole which is used for drug allergy detection and is coated with drugs and a colorific indicator. The colorific indicator is alamar blue. Due to the fact that the bacterium increasing liquid of the Edwardsiella tarda is formed by adding fish meat peptone, Ca2+ and Mg2+, and adjusting concentration of sodium chloride (NaCl) on the basis of an MH culture medium, growth of the Edwardsiella tarda can be effectively improved, the bacterium increasing liquid is enabled to reach required concentration in a short time, and time of the drug allergy detection is shortened. Meanwhile, due to the facts that a blue dyestuff namely the alamar blue is used, and the alamar blue is safe and nontoxic to cells, the Edwardsiella tarda rapid drug allergy detection kit is free from toxic action to the cells, the alamar blue is directly coated in a drug allergy detection plate micro hole and is incapable of influencing the growth of bacteria, detection operation is enabled to be simple, convenient and rapid, and multiplication trends of the bacteria can be continuously monitored.

Description

technical field [0001] The invention belongs to the technical field of in vitro diagnosis of aquatic pathogenic microorganisms, and in particular relates to a rapid drug sensitivity detection kit for aquatic animal pathogenic bacteria Edwardsiella tarda. Background technique [0002] With the rapid development of aquaculture in recent years, the problem of aquaculture animal diseases has become increasingly prominent, among which bacterial diseases have caused huge losses to the fishery economy worldwide. Edwardsiella tarda is an important pathogenic bacterium of aquatic animals, which can infect a variety of fish including freshwater and seawater, and can cause a large number of deaths in a short period of time. At present, in the process of aquaculture production, the use of antibiotics is still an important means to solve the bacterial diseases of aquaculture animals. , the species and quantity of antibiotic-resistant microorganisms in aquatic products have been continuo...

AI Technical Summary

Problems solved by technology

[0003] At present, the drug susceptibility testing methods mainly use the K-B disk method and the broth dilution method. The K-B disk method generally must be carried out under the conditions of a professional microbiological laboratory, and personnel with professional knowledge are required to complete the operation. Large amount, long cycle, etc., and the minimum inhibitory concentration (MIC) value of the drug cannot be obtained

Although the broth dilution method can determine the MIC value of the drug, the experimental cycle is long, sometimes requires professional equipment, and the interpretation of the results is not intuitive enough

Therefore, the above two methods are not suitable for application and promotion in aquaculture production.

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