Preparation method of fungal disease prevention metabolite

A technology of metabolites and fungal diseases, applied in the field of microorganisms, can solve problems such as insecurity

Inactive Publication Date: 2013-05-01
CHINA JILIANG UNIV
View PDF7 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide an endophytic actinomycete isolated and screened out from Chinese wolfberry for the deficiency that chemical pesticides are unsafe and the types

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of fungal disease prevention metabolite
  • Preparation method of fungal disease prevention metabolite
  • Preparation method of fungal disease prevention metabolite

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: (isolation and screening of Streptomyces shioya 107)

[0019] The present invention collected Chinese wolfberry stems in Hangzhou, Zhejiang in July 2012, and obtained Streptomyces shiyaya from them through the steps of surface disinfection, endophyte isolation, cultivation, fermentation, activity determination, and screening of active strains of pathogenic bacteria that inhibit plant fungal diseases. 107. Save.

[0020] Determination of antibacterial activity of Streptomyces shioya 107: Take 1mL of the fermentation supernatant filtered through a 0.22μm membrane in a sterile petri dish, mix it with 9mL of PDA medium cooled to 50°C, and place on each medium plane after cooling Put 1 rice sheath blight (Thanatephorus cucumeris) or cucumber wilt (Fusarium oxysporum.sp.cucumebrium Owen) or cucumber blight (Rhizoctonia solani) or watermelon wilt (F.oxysporum f.sp. niveum) or tomato gray mold (Botrytis cinerea) and other test bacteria cakes, the cakes are connec...

Embodiment 2

[0023] Embodiment 2: (the preparation method of Streptomyces shioya 107 fermentation metabolites)

[0024] Follow these steps:

[0025] (1) Activation culture of strains: move the isolated and preserved Streptomyces shioya 107 strains to a potato dextrose PDA solid plate, and cultivate them at 28°C±1°C until the inclined surface of the test tube is covered with spores for inoculation;

[0026] (2) Fermentation culture: the fermentation culture liquid contains 20g of soybean cake powder, 15g of corn flour, 10g of bran, 5g of peptone in every 1000mL, (NH 4 ) 2 SO 4 5g, CaCO 3 5g, MgSO 4 10g, 60mL of fermentation broth per 300mL Erlenmeyer bottle; after preparation, sterilize at 121°C for 20min, cool to 40°C, pick a spore of Streptomyces platinumensis 107 under sterile conditions and inoculate at 28°C±1°C , the shaker speed is 180 rpm, and the fermentation culture is 128h;

[0027] (3) Extraction of fermented product: After the fermentation is completed, the fermented li...

Embodiment 3

[0029] Embodiment 3: (the inhibitory effect of Streptomyces shioya 107 metabolites to rice sheath blight, cucumber solanum blight, tomato Botrytis cinerea, cucumber fusarium wilt and watermelon wilt)

[0030] (1) Accurately weigh 0.20 g of the metabolite of Streptomyces shioya 107, add 10 mL of sterile water, dissolve it fully by ultrasonic vibration, and finally prepare a mother solution with a concentration of 20 g / L;

[0031] (2) Determination of antibacterial activity: Take the above-mentioned mother liquor and dilute it into solutions with a concentration of 2g / L and 1g / L respectively, put 1mL of each concentration solution in a sterile petri dish, and quickly mix it with 9mL of PDA medium cooled to 50°C. Evenly (the final concentration of the metabolites of Streptomyces shioya 107 is 200mg / L and 100mg / L), after cooling, put a 4mm diameter of rice sheath blight or cucumber solanum blight or tomato ash on each medium plane Bacteria cakes of mildew or cucumber wilt bacteria...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Diameteraaaaaaaaaa
Login to view more

Abstract

The invention relates to the field of microbial technology. Chinese holly endophyte is separated through an endophyte microbe isolation technology from a Chinese holly plant living body collected from Hangzhou, Zhejiang province, is identified through microorganism taxonomy as Streptomyces sioyaensis, and is named as Streptomyces sioyaensis 107. The bacterial strain has already been conserved in China General Microbiological Culture Collection Center on the conservation date of December 12th, 2012 and the conservation register number of CGMCC No.6984. The most significant feature of the preparation method is that the bacterial strain liquid is fermented to produce an active metabolite which has an inhibition action on plant fungal disease pathogenic bacteria like Rhizoctonia solani and Thanatephorus cucumeris and is an important agricultural microbial resource.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to the cultivation of Streptomyces sioyaensis (Streptomyces sioyaensis) and the application of its metabolites for biocontrol endophytic actinomycetes. Background technique [0002] There are a large number of endophytes in plants. Endophytes were first discovered in pastures, and later found in woody plants, herbs, grasses, mosses, lichens, seaweeds and other plants. So far, studies have shown that plant endophytes can enhance the host's disease resistance, improve plant productivity, and resist stress and insects. These functions are due to the reproduction and growth of endophytes in plants and the production of biologically active secondary metabolites in vivo. Therefore, endophytes may become potential biological control agents in biological control, and have important uses in the development of ecological agriculture and biopesticides. In recent years, plant endophyte...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01N63/02A01P3/00
Inventor 俞晓平申屠旭萍汤谷刘叶丹陈昳丽俞叶微曾惠娟
Owner CHINA JILIANG UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap