Potentiometric sensing electrode for testing adrenergic agonist and sensor thereof

A receptor agonist and sensing electrode technology, applied in instruments, measuring devices, scientific instruments, etc., can solve the problems of inability to realize simple replacement of molecularly imprinted membranes, high cost of preparation of molecularly imprinted membrane electrodes, and difficulty in detecting a large number of samples , to achieve the effect of improving repeatability and accuracy, simple processing method and simple structure

Inactive Publication Date: 2013-05-01
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the working electrode is a titanium sheet with platinum particles with micro-nano structure, the cost is high, the operation is cumbersome when preparing the molecularly imprinted membrane on the surface of the working electrode, the electrode processing process is complicated, and the working electrode has a complex structure and different detection methods. Simple replacement of molecularly imprinted membranes cannot be achieved when samples are taken, so the testing process is complicated
In short, the preparation cost of molecularly imprinted membrane electrode is high, the preparation time is long, and the detection sensitivity is not high, it is difficult to realize the detection of a large number of samples, so it has great limitations in practical application

Method used

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  • Potentiometric sensing electrode for testing adrenergic agonist and sensor thereof

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preparation example Construction

[0045] The preparation method of the molecularly imprinted membrane for detecting adrenergic receptor agonists and the assembly of the potential type sensing electrode and the sensor involved in the present invention include the following steps:

[0046] Step 1, preparation of adrenoceptor agonist molecularly imprinted membrane: the microporous filter membrane is punched out with a puncher according to the specifications of the potential-type electrode cap to prepare a membrane of a corresponding size for use. A certain amount of adrenergic receptor agonist template molecules and functional monomers are weighed and dissolved in a solvent, and a cross-linking agent is added to react overnight under stirring conditions to form a pre-polymerization system. The next day, add adhesive water-based polyurethane and initiator, mix well by ultrasonic vibration, and blow nitrogen to remove oxygen. Spread the microporous filter membrane on a square glass slide, and then add the above-men...

Embodiment 1

[0053] The structure diagram of the potential type sensing electrode and sensor for detecting adrenoceptor agonist of the present invention is as follows figure 1 shown. Such as figure 1 As shown in A, the potentiometric sensing working electrode is composed of an insulating sleeve 1, an electrode wire 2, a platinum sheet electrode 3, an electrode cap 4, and a molecularly imprinted film 5. The potentiometric sensor includes the above-mentioned potentiometric sensing working electrode, the counter electrode, the second platinum sheet electrode 6 , the second electrode wire 8 , and a potentiometer 7 .

[0054] The insulating sleeve 1 is made of plexiglass polymethyl methacrylate. The length is 60mm, the tube wall thickness is 1.5mm, and the outer diameter is 8mm.

[0055] The electrode wire 2 is an ordinary copper wire with an insulating outer layer, and the cross-sectional specification of the wire is 1.5 mm.

[0056] The platinum sheet electrode 3 has a platinum purity of ...

Embodiment 2

[0066] This embodiment mainly relates to the testing method of the potentiometric sensor, and the potentiometric sensor used in this embodiment is the same as that in Embodiment 1.

[0067] Taking the detection of ractopamine residues in cattle urine samples as an example, a potential sensor for detecting adrenergic receptor agonists based on molecularly imprinted membranes is used for detection. The specific steps are as follows:

[0068] Urine sample pretreatment: take 5 cow urine samples confirmed by high performance liquid chromatography-mass spectrometry to not contain ractopamine, use a 10ml pipette to accurately pipette 5ml of each sample into a 15ml centrifuge tube, add 1ml containing 100μg / L ractopamine standard solution, then add 10ml methanol, oscillate and mix for 20min, centrifuge at 3000r / min for 10min, pipette the supernatant through a 0.2μm filter membrane for later use, repeat the previous operation with 10ml methanol for the remaining precipitate, and take th...

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Abstract

The invention discloses a potentiometric sensing electrode for testing an adrenergic agonist and a sensor thereof. The sensing electrode comprises an electrode cable, a platinum electrode, an insulating sleeve, an electrode cap, a molecular imprinting membrane and a potential electrode solution; the platinum electrode is arranged in the insulating sleeve, and the electrode cable is drawn out from the upper end portion of the insulating sleeve; an end cap of the electrode cap is in an annular hollow structure, and an inner circle area on the inner side of the end cap is covered with the molecular imprinting membrane provided with a molecular imprinting pore structure; the outer side on the lower end portion of the insulating sleeve is closely sleeved with the electrode cap to fix the molecular imprinting membrane between the electrode cap and the insulating sleeve; and the potential electrode solution immersing at least the lower end of the platinum electrode is poured in the insulating sleeve. According to the potentiometric sensing electrode for detecting the adrenergic agonist and the sensor thereof, the potentiometric sensing electrode uses the molecular imprinting membrane with high peculiarity as a receptor of an electrochemical sensor and has the advantages that the test sensitivity is high, the operation is simple, the sensing electrode is convenient to carry, and the like, and field tests of adrenergic agonists are achieved.

Description

technical field [0001] The invention relates to a preparation method of a sensing electrode for specifically detecting prohibited veterinary drug adrenergic receptor agonists in the field of detection technology, in particular to a potential-type sensing electrode for detecting adrenergic receptor agonists and a sensor thereof. Background technique [0002] Adrenergic receptor agonists, also known as β2 receptor agonists, mainly include clenbuterol hydrochloride, salbutamol and ractopamine, etc., and are mainly used clinically to treat bronchial asthma in humans and animals. In the 1980s, my country applied adrenal receptor agonists to feed additives. Its main function is to promote intracellular fat metabolism, significantly increase the lean meat rate of animal carcasses, and improve feed conversion rate at the same time. The abuse of this substance in the feed industry has successively led to adrenergic receptor agonist poisoning in many countries and regions, mainly invo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/30G01N27/333
Inventor 刘国艳柴春彦
Owner SHANGHAI JIAO TONG UNIV
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