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Graphene oxide drug carrier as well as preparation method and application thereof

A graphene and oxide stone technology, which is applied in drug combinations, pharmaceutical formulations, inactive components of polymer compounds, etc., can solve the problems of drug delivery to target tissues, complex preparation process, high cytotoxicity, etc., to reduce toxic side effects, Slow release rate, easy to prepare effect

Inactive Publication Date: 2013-05-22
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Traditional drug carriers often have problems such as low drug loading, complicated preparation process, poor stability, and high cytotoxicity.
Also, the large size of traditional drug carriers makes it difficult to deliver drugs to target tissues via mucosal or systemic circulation

Method used

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  • Graphene oxide drug carrier as well as preparation method and application thereof
  • Graphene oxide drug carrier as well as preparation method and application thereof
  • Graphene oxide drug carrier as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Preparation of Graphene Oxide (GO)

[0030] Add 0.2 g carbon black to 50 ml HNO 3 (25 ml concentrated HNO 3 Add 25 ml of water), heat and reflux at 130°C for 24 h, cool to room temperature and centrifuge at 8000 rpm for 10 min, take the supernatant and heat to 150°C to evaporate to obtain a reddish-brown solid, add 4 ml of water to the solid and mix well to obtain a reddish-brown solution , Dialyzed with a dialysis bag with a molecular weight of 3500 for 24 h, took out the liquid in the dialysis bag and added Na 2 CO 3 The pH of the solution was adjusted to neutral, and the inner solution was dialyzed for 3 days to obtain a graphene oxide solution, which was stored at 4°C for later use.

Embodiment 2

[0032] Preparation, stability and cytotoxicity of graphene oxide-human serum albumin (GO-HSA)

[0033] After GO and HSA were mixed and shaken at a mass ratio of 1:5 for 1 h, GO-HSA was placed in H 2 O, PBS, 1640 cell culture medium, and calf serum were used to make the final concentration 400 μg / ml, and after centrifugation at 12,000 rpm for 10 min, no precipitation was observed, and GO-HSA in each solvent was still uniformly dispersed and stable. HeLa cells were seeded in a 96-well plate. After 24 h, GO-HSA was added to the well plate to form a concentration gradient. At the same time, culture medium was added for cultivation. After 24 h, MTT dye was added, and after 3.5 h, DMSO was added to mix well. After 10 min, Read the OD value with a plate reader. The result is as figure 1 As shown, it can be seen that even high concentrations of GO-HSA are essentially non-toxic to cells.

Embodiment 3

[0035] Preparation and cytotoxicity of graphene oxide-human serum albumin-doxorubicin (GO-HSA-DOX)

[0036] GO-HSA and DOX were mixed and shaken at a mass ratio of 1:5:1 for 1 h, then ultra-filtered to remove unbound DOX, the precipitate was re-dissolved evenly with PBS, and placed in a 4°C refrigerator for later use. Seed HeLa cells in a 96-well plate, add GO-HSA-DOX to the well plate to form a concentration gradient after 24 hours, and add culture medium at the same time, add MTT dye after 24 hours, add DMSO after 3.5 hours and mix well, 10min Then read the OD value with a plate reader. The result is as figure 2 As shown, it can be seen that GO-HSA-DOX has a better effect on killing cancer cells than DOX alone.

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Abstract

The invention discloses a graphene oxide drug carrier as well as a preparation method and an application thereof. A monolayer or multilayer graphene oxide of human serum albumin is combined to serve as a transport carrier of the drug, and active ingredients of the drug are loaded by absorption. The drug carrier is simple in preparation, good in biocompatibility and very low in cytotoxicity, so that the drug carrier has a wide application prospect in a clinical application, and particularly in malignant tumor treatment aspect.

Description

technical field [0001] The invention belongs to the field of nano drug carriers, and in particular relates to a graphene oxide drug carrier and its preparation method and application. Background technique [0002] A drug carrier refers to a system that can load drugs, change the way the drug enters the body and distribute in the body, control the release rate of the drug, and deliver the drug to the target organ. Nanotechnology is one of the current research hotspots, and its core is to use the special properties of nanomaterials to achieve functions and uses that cannot be achieved by general materials. Nanomaterials can freely enter cells and accumulate in tumor tissues. Utilizing the particularity of nanomaterials and using them in combination with drugs can reduce the dosage of drugs and make them have corresponding targeting. Human serum albumin is a single-chain aglycosyl protein composed of 585 amino acid residues. It has physiological activities such as binding and...

Claims

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Application Information

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IPC IPC(8): A61K47/42A61K47/04A61K45/00A61P35/00
Inventor 杨黄浩靳贵晓李娟吴灵洁郭珊珊
Owner FUZHOU UNIV
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