Graphene oxide drug carrier as well as preparation method and application thereof
A graphene and oxide stone technology, which is applied in drug combinations, pharmaceutical formulations, inactive components of polymer compounds, etc., can solve the problems of drug delivery to target tissues, complex preparation process, high cytotoxicity, etc., to reduce toxic side effects, Slow release rate, easy to prepare effect
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Embodiment 1
[0029] Preparation of Graphene Oxide (GO)
[0030] Add 0.2 g carbon black to 50 ml HNO 3 (25 ml concentrated HNO 3 Add 25 ml of water), heat and reflux at 130°C for 24 h, cool to room temperature and centrifuge at 8000 rpm for 10 min, take the supernatant and heat to 150°C to evaporate to obtain a reddish-brown solid, add 4 ml of water to the solid and mix well to obtain a reddish-brown solution , Dialyzed with a dialysis bag with a molecular weight of 3500 for 24 h, took out the liquid in the dialysis bag and added Na 2 CO 3 The pH of the solution was adjusted to neutral, and the inner solution was dialyzed for 3 days to obtain a graphene oxide solution, which was stored at 4°C for later use.
Embodiment 2
[0032] Preparation, stability and cytotoxicity of graphene oxide-human serum albumin (GO-HSA)
[0033] After GO and HSA were mixed and shaken at a mass ratio of 1:5 for 1 h, GO-HSA was placed in H 2 O, PBS, 1640 cell culture medium, and calf serum were used to make the final concentration 400 μg / ml, and after centrifugation at 12,000 rpm for 10 min, no precipitation was observed, and GO-HSA in each solvent was still uniformly dispersed and stable. HeLa cells were seeded in a 96-well plate. After 24 h, GO-HSA was added to the well plate to form a concentration gradient. At the same time, culture medium was added for cultivation. After 24 h, MTT dye was added, and after 3.5 h, DMSO was added to mix well. After 10 min, Read the OD value with a plate reader. The result is as figure 1 As shown, it can be seen that even high concentrations of GO-HSA are essentially non-toxic to cells.
Embodiment 3
[0035] Preparation and cytotoxicity of graphene oxide-human serum albumin-doxorubicin (GO-HSA-DOX)
[0036] GO-HSA and DOX were mixed and shaken at a mass ratio of 1:5:1 for 1 h, then ultra-filtered to remove unbound DOX, the precipitate was re-dissolved evenly with PBS, and placed in a 4°C refrigerator for later use. Seed HeLa cells in a 96-well plate, add GO-HSA-DOX to the well plate to form a concentration gradient after 24 hours, and add culture medium at the same time, add MTT dye after 24 hours, add DMSO after 3.5 hours and mix well, 10min Then read the OD value with a plate reader. The result is as figure 2 As shown, it can be seen that GO-HSA-DOX has a better effect on killing cancer cells than DOX alone.
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