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Piperazine degraded achromobacter strain MBPQ-CG and application thereof

A colorless bacillus and piperazine technology, applied in the field of environmental microorganisms, can solve the problems of no piperazine and other problems, achieve low production cost, good removal effect, and reduce the cost of wastewater treatment

Active Publication Date: 2013-05-22
JIANGSU NANZI ENVIRONMENTAL PROTECTION SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Biodegradation is the main way to remove amines in nature. At present, there is no research report on piperazine-degrading bacteria in China. Therefore, microbial strains that efficiently degrade piperazine are isolated from piperazine-contaminated environments, and the use of microorganisms to restore Piperazine pollution in the environment has great theoretical significance and potential application prospects

Method used

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  • Piperazine degraded achromobacter strain MBPQ-CG and application thereof
  • Piperazine degraded achromobacter strain MBPQ-CG and application thereof
  • Piperazine degraded achromobacter strain MBPQ-CG and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0024] Example 1 Isolation and Identification of Bacterial Strains

[0025] Take the piperazine-enriched bacterial solution and perform gradient dilution. The dilution is spread on the inorganic salt solid medium containing 100mg / L piperazine, and its formula is: 1.5 g K per liter 2 HPO 4 , 0.5 g KH 2 PO 4 , 0.2 g MgSO 4 ×7H 2 O, 1.0 g NaCl, 18 g agar, pH 7.0, culture at 30°C for several days. Pick a single colony to verify its degradation effect, and save a strain with higher degradation efficiency for subsequent experiments. See the picture of its colony figure 1 , identified as Colorless (Bacillus) genus ( Achromobacter sp. ); named: MBPQ-CG. Its crystal violet staining photo is as figure 2 As shown, the main biological characteristics are Gram-negative, rod-shaped, no spores, motility, and positive for catalase and oxidase. It can grow with piperazine as the only carbon and nitrogen source and completely mineralize it. The degradation rate of 250mg / L piperazi...

Embodiment 2

[0026] Example 2 Method for preparing immobilized pellets from bacterial strain MBPQ-CG

[0027] 1) Use an inoculation loop to pick a small amount of strains on the slant medium and inoculate them in LB liquid medium at 30°C and shake at 160r / min until the logarithmic phase; the LB medium is: NaCl 10.0g / L, peptone 10.0g / L, yeast powder 5.0g / L, pH7.0;

[0028] 2) Centrifuge the above cultured bacterial solution at 8000r / min for 2min, discard the supernatant, add the same volume of sterile water, shake well and centrifuge at 8000r / min for 2min, wash twice in this way, and use the same volume Suspended in sterile water to make bacterial liquid;

[0029] 3) Take 0.7ml of bacterial liquid and 20ml of 4% sodium alginate solution and mix at room temperature, then use a syringe to drop the mixture into 3% CaCl at room temperature 2 Solution, after cross-linking and calcification at 4°C for 6h to obtain calcium alginate gel immobilized beads, such as image 3 indicated, washed twic...

Embodiment 3

[0030] Example 3 Application Method of Immobilized Beads in Piperazine Degradation

[0031] Under the suitable conditions of temperature 28±2℃ and pH value of 6.8-7.2, when the piperazine influent concentration is lower than 400mg / L, add 5% immobilized microbial pellets per liter of piperazine wastewater for treatment , At the same time, the hydraulic retention time is 80h. In this case, the removal rate of piperazine is above 99%, which has a good removal effect. Specific as Figure 4 and Figure 5 As shown, among them, Figure 4 Shows the removal of piperazine for different influent concentrations, the study shows that the removal rate of piperazine decreases with the increase of influent concentration, when the influent piperazine concentration is in the range below 400mg / l , piperazine has a high removal rate, which can reach more than 99%; Figure 4 It shows that under the suitable conditions of temperature of 28±2°C and pH value of about 7.0, when the concentration o...

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Abstract

The invention provides a degradation microbial inoculum for eliminating piperazine residue, and belongs to the high technical field of biology. The strain is gram staining reaction negative strain MBPQ-CG, and is identified to be achromobacter sp., wherein the main biological characteristics are gram negative, bacilliform, asporous and non-motile, and the catalase and oxidase are positive. The strain can grow by taking piperazine as the unique carbon source and nitrogen source, and can be completely mineralized. 250mg / L of piperazine can be degraded to a level which cannot be detected in 30 hours by the strain under a shaking condition in the lab, so that the problem that the piperazine is difficult to biodegrade in waste water treatment can be solved.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a piperazine-degrading Achromobacter MBPQ-CG and an application thereof. Background technique [0002] Amine compounds can be regarded as the products obtained after the hydrogen atoms in the ammonia molecule are replaced by hydrocarbon groups. Many amine compounds are widely used in medicine, industry, agriculture and other fields. Extensive use of organic amines will inevitably pollute the environment and endanger human health. Lower amines have a strong ammonia-like odor, while trimethylamine has an unpleasant fishy smell. Lower aliphatic amines are easily soluble in water, and their solubility and odor in water decrease with the increase of molecular weight. Aromatic amines are liquids or solids with an unpleasant odour. Arylamines with simpler structures are slightly soluble in water, and more complex ones are insoluble. Aromatic amines are highly t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N11/10C12N11/04C02F3/34C12R1/025C02F101/38
Inventor 张永乐顾乔梅蔡双梅高益民
Owner JIANGSU NANZI ENVIRONMENTAL PROTECTION SCI & TECH
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