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Tissue engineering scaffold with multi-growth-factor sequential release characteristic

A tissue engineering scaffold and growth factor technology, applied in medical science, prosthesis, etc., can solve the problems of difficulty in introducing growth factors, harsh preparation process conditions, organic solvent residues, etc., and achieves simple operation, good physical and chemical properties, and low toxicity. Effect

Inactive Publication Date: 2013-06-12
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to prepare degradable porous scaffolds, the traditional preparation methods of scaffolds are complicated to operate, and the conditions of the preparation process are harsh, involving high temperature, residual organic solvents, difficulty in introducing growth factors, etc.

Method used

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  • Tissue engineering scaffold with multi-growth-factor sequential release characteristic
  • Tissue engineering scaffold with multi-growth-factor sequential release characteristic
  • Tissue engineering scaffold with multi-growth-factor sequential release characteristic

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0020] Example 1 Preparation of BMP-2-PLLA, VEGF-PLLA and bFGF-PLLA drug-loaded microspheres

[0021] (1) Dissolve 10 μg of BMP-2, VEGF and bFGF in 250 uL PBS as the inner aqueous phase.

[0022] (2) Weigh 250 mg of PLLA (molecular weight 1-100,000) and polyethylene glycol (PEG, molecular weight 400-10,000), the mass ratio of PLLA to PEG is 1:2-2:1, dissolve in 5 mL Dichloromethane was used as the oil phase (the volume ratio of the inner water phase to the oil phase was 1:20).

[0023] (3) Slowly pour the inner water phase into the oil phase and stir at high speed for 2 minutes to form colostrum (W / O).

[0024] (4) Slowly drop colostrum into 10 mL of 1 % PVA, stir at high speed for 5 minutes to form double milk (WO / W).

[0025] (5) Slowly drop double emulsion into 250mL, 0.1% PVA, stir at room temperature for 4 hours, 500 r / min, for volatile organic solvent.

[0026] (6) Centrifuge at 4000 r / min for 10 minutes to collect microspheres, wash with deionized water three times, ...

example 2

[0029] Example 2 Preparation of HA / PLGA porous scaffold containing trypsin sustained-release microspheres

[0030] (1) PLGA to be purchased (PLA:PLG=50:50, M W =100,000Da) and HA powder at a ratio of 1:4 for grinding for 8 hours, then add 10% trypsin slow-release microspheres and mix well with the powder.

[0031] (2) Accurately weigh 0.125g of the mixed powder and put it into a cylindrical mold with a diameter of 5mm for pressing. The pressure is 10MPa and the pressing time is 1min to obtain a small disc with a diameter of 5mm and a height of about 3mm. In the tetrafluoroethylene mold customized by the laboratory.

[0032](3) Put the tetrafluoroethylene mold into the autoclave, turn on the supercritical foaming device, the refrigeration system starts to work, set the reaction temperature to 39°C, and the pressure to 8Mpa, turn on the high-pressure plunger pump, constant temperature water bath box and drum drying box, the reaction kettle is boosted and heated. Adjust the ve...

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Abstract

The invention provides a tissue engineering scaffold with multi-growth-factor sequential release characteristic, and belongs to the technical field of dosage form variation and preparation method. In the tissue engineering scaffold, together with the characteristics of microspheres sustained release system and scaffold, PLLA / PEG (poly-lactic acid / polyethylene glycol) sustained release microspheres of growth factors such as BMP-2 (bone morphogenetic protein), VEGF (vascular endothelial growth factor), bFGF (basic fibroblast growth factor) are prepared respectively by the multiple emulsion method, so as to construct the tissue engineering scaffold with multi-growth-factor sequential release characteristic. The method is high in drug activity maintaining degree, low in organic solvent residue and easy to operate. The prepared microspheres are narrow in particle size distribution and have an excellent sustained release effect. The pore size distribution of the composite scaffold is 150-300mu m, the connectivity is excellent, the porosity is 76.84%, the compressive strength is 5.11MPa, and the accumulative release amount of 21 days is 60.6%. The scaffold prepared according to the invention has an application prospect in tissue engineering restoration.

Description

technical field [0001] The invention is a tissue engineering scaffold with sequential release characteristics of multiple growth factors, which belongs to the technical field of drug dosage form change and its preparation method, and in particular uses supercritical fluid technology to prepare multiple growth factors with sequential release characteristics Tissue engineering scaffolds are expected to be used for regeneration and repair of bone tissue. Background technique [0002] During the formation, development and repair of tissue-engineered bone, various growth factors are crucial to the regulation of local tissues and cells, acting on different phases and coordinating with each other. The direct use of soluble cytokines has a short half-life and requires repeated operations to supplement other obstacles. Therefore, the construction of a controlled release system for the sustained and controllable delivery of multiple growth factors to exert their maximum biological sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/26A61L27/18
Inventor 尹光福白燕李培培
Owner SICHUAN UNIV
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