Phenylurea herbicide n-demethylase gene pudma and its application

A technology of demethylase and gene, applied in application, genetic engineering, plant genetic improvement, etc., can solve problems that threaten human life and health, function damage, and excessive pesticides
CN103266117BInactive Publication Date: 2014-10-08NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2014-10-08
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a phenylurea herbicide N-demethylase gene pudmA and an application thereof. A nucleotide sequence of the phenylurea herbicide N-demethylase gene pudmA is shown in SEQ ID NO.1; the overall length is 1907bp; the content of G+C is 55.37%; a large subunit code includes 459 amino acids; a small subunit code includes 176 amino acids; and the amino acid sequence is shown in SEQ ID NO.2. The phenylurea herbicide N-demethylase gene pudmA provided by the invention can remove N-methyl of phenylurea herbicides such as isoproturon and chlortoluron, so that the herbicidal activity is removed. Therefore, the phenylurea herbicide N-demethylase gene pudmA can be used for building genetically modified crops of an anti-phenyl urea herbicide. In addition, the phenylurea herbicide N-demethylase gene pudmA and encoded protein PudmA also can be used for removing residual phenylurea herbicide in the environment.
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Description

technical field

[0001] The invention belongs to the field of application of environmental microorganisms and agriculture, and relates to a phenylurea herbicide N-demethylase gene pudmA and its application. Background technique

[0002] Pesticides have played a huge role in agricultural production, crop pest control, weed removal, and control of human and animal infectious diseases, but at the same time, they also bring serious environmental problems. Pesticide pollution directly leads to excessive pesticides in agricultural products, and product quality declines, seriously affecting On the other hand, pesticide residues in the environment are enriched in various ways, mainly in the food chain, and ultimately directly threaten human life and health; in addition, pesticide residues also indirectly lead to changes in ecosystem structure and function damage.

[0003] Phenylurea (or substituted urea) herbicides (phenylurea herbicides) such as isoproturon and chloromalon are a ser...

Claims

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