Sulphated heparan sulfated for inhibiting cell proliferation

A technology of heparan sulfate and sulfate esterification, which is used in medical preparations containing active ingredients, organic active ingredients, antitumor drugs, etc., can solve the difficulty of preparing high-purity HS, has not been widely used, and the effect of natural HS is not significant And other issues
CN103288981AInactive Publication Date: 2013-09-11SHENZHEN HEPALINK PHARMA GRP CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
SHENZHEN HEPALINK PHARMA GRP CO LTD
Publication Date
2013-09-11
Estimated Expiration
Not applicable · inactive patent

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Abstract

A heparan sulfated derivative is prepared from heparan sulfated by sulphating. A preparation method comprises: swelling heparan sulfated by anhydrous dimethyl formamide, adding a sulfur trioxide-trimethylamine complex, heating for esterification, dissolving, dialyzing, freeze-drying precipitation components of the reactant by distilled water, then dissolving by sodium chloride solution, precipitating by ethanol, centrifuging, removing a supernatant, drying, and then obtaining sulphated heparan sulfated. The heparan sulfated derivative can inhibit activities of tumor cells and umbilical vein endothelial cell proliferation.
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Description

technical field

[0001] The invention relates to a sulfated heparan sulfate (Heparan Sulfated, HS), its preparation method and its application in inhibiting cell proliferation. Background technique

[0002] HS is a complex polysaccharide compound widely present on the cell surface and in the extracellular matrix (ECM). Its structure is as figure 1 [Sanaullah Khan, Elizabeth Rodriguez, Rima Patel, Jayesh Gor, Barbara Mulloy, Stephen J. Perkins. The Solution Structure of Heparan Sulfate Differs from That of Heparin. J Biol Chem. 2011, 286(28): 24842–24854], of which Glucosamine residues can be modified by N-acetylation, N- and O-sulfation, and glucuronic acid residues can be isomerized to iduronic acid (IdoA) residues.

[0003] Tumor invasion and metastasis are the most dangerous stages in the process of tumor occurrence and evolution, and they are also signs of malignant development of tumors. More than 80% of clinical cancer patients die from tumor invasion and metastasis. ...

Claims

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