Novel sea asparagus triterpenoid saponin reducing compound as well as preparation method and application thereof
A technology of triterpene saponins and compounds, applied in the field of new compounds of sea asparagus nortriterpene saponins and their preparation
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Embodiment 1
[0020] Example 1 Preparation of compound
[0021] Freshly picked 22.6 kg of sea asparagus above ground, crushed with a homogenizer, placed in a 20 L volumetric flask, soaked with 10 L of 80% acetone aqueous solution, and left to stand for 24 hours; the extract was filtered with a Buchner funnel , The filtered sea asparagus residue was extracted twice with 10 L 80% acetone aqueous solution, and the extract was concentrated under reduced pressure to no acetone; extracted with an equal volume of ethyl acetate three times to remove most of the fat-soluble components, the raffinate Extract 3 times with an equal volume of methanol, filter, take the upper liquid, and concentrate to dryness to obtain a methanol extract.
[0022] The methanol extract (85 g) was mixed with 60 g silica gel dry method, and then loaded on an atmospheric column equipped with 200 g silica gel (300-400 mesh), followed by petroleum ether-acetone (50:1, 20:1, 10:1). , 5:1, 1:1, 1:10, 1:50, 1:100, 0:1), acetone-meth...
Embodiment 2
[0032] Example 2 Test of anti-tumor activity
[0033] 1. Experimental method and reagent preparation
[0034] MTT method was used to determine the inhibitory effects of two compounds on A549 and hepG2 cells: A549 (human lung cancer cells) and hepG2 (human liver cancer cells) in logarithmic growth phase were taken, and the concentration of the cell suspension was adjusted. 100 μL per well was added to 96-well cells In the culture plate, spread the plate so that the cell density to be tested is 3000 / well, and the 2 , 37 ℃ incubator for 4 h; the samples to be tested are set with 5 concentration gradients of 0.1, 1, 10, 25, 50 μM, each concentration is set 5 parallel, and the zero adjustment hole and the control well are set at the same time. Add 100 μL of sample solution or blank solution (cells, drug lysis medium, cell culture solution) to the wells, and add 20 μL of MTT solution (5 mg / mL, PBS solution configuration) to each well after culturing for 72 hours. Continue to incubate for...
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