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Nanometer material probe system and detection method for tubercle bacillus detection

A Mycobacterium tuberculosis, nanoprobe technology

Active Publication Date: 2013-09-25
SHANGHAI PULMONARY HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, although the study used the amplification effect of biotin avidin to try to improve the detection sensitivity, even if the instrument was used to detect the fluorescent signal, the detection limit was only 1000 bacteria, slightly higher than the detection limit of the smear, but it was not The detection effect of culture has not been achieved, and 4 different binding molecules need to be added, there are many influencing factors, and the operation steps are cumbersome, which does not fully reflect the advantages of nanotechnology

Method used

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  • Nanometer material probe system and detection method for tubercle bacillus detection
  • Nanometer material probe system and detection method for tubercle bacillus detection
  • Nanometer material probe system and detection method for tubercle bacillus detection

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Step 1, preparation of functionalized nano-magnetic beads

[0057] In the presence of sodium citrate, ferric ions were reduced with ethylene glycol, and reacted at 200°C and 2 atmospheres to prepare magnetic microspheres.

[0058] The silicon oxide shell layer is prepared by a sol-gel method, the magnetic microspheres are coated, and then the amino groups are modified on the surface of the magnetic microspheres by using the hydroxyl groups on the surface of the silicon oxide and the carboxyl groups of the silane coupling agent with amino groups.

[0059] Reaction with acid anhydride and amino group to obtain magnetic microspheres with carboxylation on the surface.

[0060] Step 2, preparation of integrated nanomaterials

[0061] First, the sol-gel method is used to synthesize silicon oxide particles, and the powder is obtained by freeze-drying. The silicon oxide particles are stabilized at the water / toluene two-phase interface by stirring, and then the silane coupling ...

Embodiment 2

[0074] Step 1, preparation of functionalized nano-magnetic beads

[0075] In the presence of succinic acid, trivalent iron ions were reduced with glycerol, and reacted at 200°C and 2.7 atmospheric pressure to prepare magnetic microspheres.

[0076]The silicon oxide shell layer is prepared by a sol-gel method, the magnetic microspheres are coated, and then the amino groups are modified on the surface of the magnetic microspheres by using the hydroxyl groups on the surface of the silicon oxide and the carboxyl groups of the silane coupling agent with amino groups.

[0077] Reaction with acid anhydride and amino group to obtain magnetic microspheres with carboxylation on the surface.

[0078] Step 2, preparation of integrated nanomaterials

[0079] First, the sol-gel method is used to synthesize silica particles, and the powder is obtained by freeze-drying. The silica particles are stabilized at the water / chlorobenzene two-phase interface by stirring, and then the silane couplin...

Embodiment 3

[0089] Step 1, preparation of functionalized nano-magnetic beads

[0090] In the presence of sodium citrate, ferric ions were reduced with isopropanol and reacted at 200°C and 1.2 atmospheric pressure to prepare magnetic microspheres.

[0091] The silicon oxide shell layer is prepared by a sol-gel method, the magnetic microspheres are coated, and then the amino groups are modified on the surface of the magnetic microspheres by using the hydroxyl groups on the surface of the silicon oxide and the carboxyl groups of the silane coupling agent with amino groups.

[0092] Reaction with acid anhydride and amino group to obtain magnetic microspheres with carboxylation on the surface.

[0093] Step 2, preparation of integrated nanomaterials

[0094] First, the sol-gel method is used to synthesize silicon oxide particles, and the powder is obtained by freeze-drying. The silicon oxide particles are stabilized at the water / xylene phase interface by stirring, and then the silane coupling...

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Abstract

The present invention provides a nanometer probe system for tubercle bacillus detection, and a preparation method for the nanometer probe, and further provides a tubercle bacillus detection method based on the nanometer probe system and the preparation method. The nanometer probe system comprises an integrated nanometer material probe and immunomagnetic beads, wherein the integrated nanometer material probe, the immunomagnetic beads and tubercle bacillus thallus are combined through specific ligands to form a tertiary complex, a magnetic field effect is adopted to rapidly and accurately separate and concentrate the tubercle bacillus and the integrated nanometer probe, a fluorescence signal on the integrated probe is detected, and the detection signal can be significantly amplified through magnetic enrichment and quantity difference between polypeptide sites and fluorescence quantum dots on the integrated nanometer probe so as to achieve accurate, highly sensitive and specific tubercle bacillus detection.

Description

technical field [0001] The invention relates to a nano material, in particular to a magnetic nano microsphere for detecting Mycobacterium tuberculosis, a nano material, a preparation method thereof, and a method for detecting Mycobacterium tuberculosis based on the magnetic nano microsphere and the integrated nano material. Background technique [0002] Tuberculosis is an infectious disease that seriously endangers human health, and it is also a public health and social problem of global concern. Early diagnosis is very important for the discovery and treatment of tuberculosis. At present, there are three main types of tuberculosis laboratory diagnosis methods, namely bacteriological examination, molecular biological examination and immunological examination, among which bacteriological examination (including smear method and culture method) is The "gold standard" for TB diagnosis. [0003] However, the existing smear examinations, including direct smear method, bacterial c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11C12N15/10G01N33/68G01N33/533C12R1/32
Inventor 杨华胡忠义秦莲花刘忠华马慧
Owner SHANGHAI PULMONARY HOSPITAL
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